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34results about How to "Overcome the defects of low sensitivity and poor repeatability of test results" patented technology

Liquid phase chip for CYP19A1 gene SNP (Single Nucleotide Polymorphism) detection and detection method thereof

The invention discloses a liquid phase chip for CYP19A1 gene SNP (Single Nucleotide Polymorphism) detection, which comprises wild type and mutant type specific ASPE primers designed targeting CYP19A1 gene SNPs of rs4646, rs10046C>T, rs700519C>T, rs1870050C>A, hCV1664178A>C, rs12900137G>C, rs730154G>A, rs936306T>C and rs1902586A>G, microballoon spheres respectively coated with specific anti-tag sequences and primers used for amplifying CYP19A1 gene SNPs with target sequences to be detected. Each ASPE primer comprises a tag sequence at 5' end and a specific primer sequence at 3' end, wherein the specific primers are selected from SEQ ID NO. 19-36 and the tag sequences are selected from SEQ ID NO.1-18; and the anti-tag sequences can be correspondingly in complementary pairing with the tag sequences. The invention also discloses a CYP19A1 gene SNP detection method. The coincidence rate of the detection method provided by the invention and a sequencing method is as high as 100%; and the prepared liquid phase chip for CYP19A1 gene SNP (Single Nucleotide Polymorphism) detection has excellent signal-to-noise rate, and basically no cross reaction exists between a design probe and the anti-tag sequences.
Owner:SUREXAM BIO TECH

NRAS gene mutation detection specificity primer and liquid chip thereof

The invention discloses an NRAS gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21 and / or SEQ ID NO.22 focused on a Codon12 site, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26 and / or SEQ ID NO.27 focused on a Codon13 site, SEQ ID NO.28 and SEQ ID NO.29 focused on a Codon18 site, and / or SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33 and / or SEQ ID NO.34 focused on a Codon61 site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.
Owner:SUREXAM BIO TECH

THADA gene mutation detection specific primer and liquid phase chip

The invention discloses a THADA gene mutation detection liquid phase chip and a specific primer. The liquid phase chip mainly comprises: an ASPE primer composed of a tag sequence at 5'-terminal and specific primer sequences against the mutation site of a target gene at 3'-terminal, wherein the specific primer sequences comprise SEQ ID NO.13 and SEQ ID NO.14 against an A109G site, SEQ ID NO.15 and SEQ ID NO.16 against a T156C site, SEQ ID NO.17 and SEQ ID NO.18 against an A89C site, SEQ ID NO.19 and SEQ ID NO.20 against a G153A site, SEQ ID NO.21 and SEQ ID NO.22 against a G162T site, and / or SEQ ID NO.23 and SEQ ID NO.24 against a C80T site; a microsphere coated with an anti-tag sequence; and an amplimer. The coincidence rate between the detection result of the detection liquid phase chip and a sequencing method is 100%, and the wild and mutant parallel detection of a plurality of mutation sites can be realized.
Owner:SUREXAM BIO TECH

SNP (Single Nucleotide Polymorphism) detection liquid phase chip for KIF6 and apo E genes and chromosome 9p21 section

The invention discloses an SNP (Single Nucleotide Polymorphism) detection liquid phase chip for KIF6 gene, mainly comprising an ASPE (Application Solid Phase Extraction) primer pair, microballons and an amplification primer, wherein each ASPE primer consists of a 5'-end tag sequence and 3'-end specific primers aiming at T2155C SNP locus; the 3'-end specific primers comprise SEQID NO.23 and SEQ IDNO.24; the tag sequence is selected from SEQID NO.1 to SEQID NO.18; and the microballons are respectively coated by a specific anti-tag sequence and have codes in different colors. The invention also discloses the SNP detection liquid phase chip for apoE and KIF6 genes and the chromosome 9p21 section. The occlusion rate of the detection method and the sequencing method which are provided in the invention is as high as 100 percent. The prepared SNP detection liquid phase chip for the apoE and KIF6 genes and the chromosome 9p21 section has favorable single-noise ratio.
Owner:SUREXAM BIO TECH

ATM gene mutation detection specific primer and liquid chip

The invention discloses an ATM gene mutation detection specific primer and a liquid chip. The liquid chip mainly comprises ASPE primers consisting of tag sequences at a 5' end and specific primer sequences aiming at target gene mutation sites at a 3' end, microspheres coated with anti-tag sequences and an amplification primer, wherein the specific primer sequences are as follows: SEQ ID NO.13 and SEQ ID NO.14 which aim at a T2572C site, SEQ ID NO.15 and SEQ ID NO.16 which aim at an A7325C site, SEQ ID NO.17 and SEQ ID NO.18 which aim at a G7328A site, SEQ ID NO.19 and SEQ ID NO.20 which aim at a C9022T site, SEQ ID NO.21 and SEQ ID NO.22 which aim at a G9023A site, and / or SEQ ID NO.23 and SEQ ID NO.24 which aim at a C9139T site. The detection liquid chip provided by the invention has the advantages that the matching rate between the detection result and a sequencing method is up to 100%, and the wild-type and mutant parallel detection of multiple mutant sites can be realized.
Owner:SUREXAM BIO TECH

BAT3 gene mutation detection specific primer and liquid phase chip

The invention discloses a BAT3 gene mutation detection liquid phase chip and a specific primer. The liquid phase chip mainly comprises: an ASPE primer composed of a tag sequence at 5'-terminal and specific primer sequences against the mutation site of a target gene at 3'-terminal, wherein the specific primer sequences comprise SEQ ID NO.1 and SEQ ID NO.2 against an A131C site, and / or SEQ ID NO.3 and SEQ ID NO.4 against a G96A site; a microsphere coated with an anti-tag sequence; and an amplimer. The coincidence rate between the detection result of the detection liquid phase chip and a sequencing method is 100%, and the wild and mutant parallel detection of a plurality of mutation sites can be realized.
Owner:SUREXAM BIO TECH

Specific primmer and liquid phase chip for SLCO1B1 gene SNP detection

The invention discloses a specific primmer and a liquid phase chip for solute carrier organic anion transporter 1B1 (SLCO1B1) gene signal nucleotide polymorphism (SNP) detection. The liquid phase chip mainly comprises allele specific primer extension (ASPE) primer pairs, microspheres and amplifiers, wherein each ASPE primer consists of a tag sequence at a 5' end and specific primers at a 3' end aiming at an SNP locus; the specific primers comprise SEQ ID NO.11 and SEQ ID NO.12 aiming at T521C SNP locus, SEQ ID NO.13 and SEQ ID NO.14 aiming at T89595C SNP locus, SEQ ID NO.15 and SEQ ID NO.16 aiming at A388G SNP locus, SEQ ID NO.17 and SEQ ID NO.18 aiming at C463A SNP locus, and / or SEQ ID NO.19 and SEQ ID NO.20 aiming at A1929C SNP locus; and the tag sequence is selected from SEQ ID NO.1 to SEQ ID NO.10. The coincidence rate of the liquid phase chip provided by the invention and the detection result of a sequencing method reaches 100 percent. And the prepared liquid phase chip for the SLCO1B1 gene SNP detection has high signal-to-noise ratio.
Owner:SUREXAM BIO TECH

Specific primers and liquid phase chip for detecting polymorphism of cyckin-dependent kinase 5 regulatorysubunit-associated protein 1-like 1(CDKAL1) gene

The invention discloses specific primers and a liquid phase chip for detecting the polymorphism of a CDKAL1 gene. The liquid phase chip mainly comprises specific primers consisting of a tag sequence at a 5' end and specific primer sequences of polymorphic sites of a target gene, microspheres and amplification primers, wherein the specific primer sequences are one or more pairs from SEQ ID No.9 and SEQ ID No.10 for A107C, SEQ ID No.11 and SEQ ID No.12 for G323C, SEQ ID No.13 and SEQ ID No.14 for A210G and SEQ ID No.15 and SEQ ID No.16 for A75G; the tag sequence may be a sequence from SEQ ID No.1 to SEQ ID No.8. The prepared liquid phase chip for detecting the polymorphism of the CDKAL1 gene has a very high signal-to-noise ratio, and the cross reaction of a designed probe and an anti-tae sequence is prevented basically.
Owner:SUREXAM BIO TECH

VHL (Von Hippel Lindau) genetic mutation detection specific primer and liquid phase chip

The invention discloses a VHL (Von Hippel Lindau) genetic mutation detection specific primer and a liquid phase chip. The liquid phase chip mainly comprises ASPE (Allele Specific Primer Extension) primers, microspheres coated by an anti-tag sequence, and amplimers, wherein the ASPE primers consist of tag sequences at a 5' end and specific primer sequences specific to target genetic mutation sites at a 3' end; and the specific primer sequences are: SEQ ID NO.13 and SEQ ID NO.14 specific to a T240A site; SEQ ID NO.15 and SEQ ID NO.16 specific to a T254C site; SEQ ID NO.17 and SEQ ID NO.18 specific to a T266A site; SEQ ID NO.19 and SEQ ID NO.20 specific to a T286T site; SEQ ID NO.21 and SEQ ID NO.22 specific to a G388C site; and / or SEQ ID NO.23 and SEQ ID NO.24 specific to a 444delT site. The matching rate between a detection result of the liquid phase chip provided by the invention and a sequencing method is up to 100 percent, and wild and mutant parallel detection of a plurality of mutant sites is realized.
Owner:SUREXAM BIO TECH

ABCC (ATP (adenosine triphosphate)-binding cassette, sub-family C)1 gene mutation detection specific primers and liquid chip

The invention discloses an ABCC (ATP (adenosine triphosphate)-binding cassette, sub-family C)1 gene mutation detection liquid chip and specific primers. The liquid chip mainly comprises each ASPE (allele specific primer extension) primer formed by tag sequences at 5' terminal and specific primer sequences aiming at target gene mutation sites at 3' terminal, microspheres coated by anti-tag sequences as well as amplification primers, wherein the specific primer sequences are SEQ ID NO.15 and SEQ ID NO.16 aiming at A866T sites, SEQ ID NO.17 and SEQ ID NO.18 aiming at C218T sites, SEQ ID NO.19 and SEQ ID NO.20 aiming at G2168A sites, SEQ ID NO.21 and SEQ ID NO.22 aiming at G3173A sites, SEQ ID NO.23 and SEQ ID NO.24 aiming at G16237754A sites, SEQ ID NO.25 and SEQ ID NO.26 aiming at C16238494T sites and / or SEQ ID NO.27 and SEQ ID NO.28 aiming at G1299T sites. The liquid chip has the advantages that the coincidence rate of the detection results of the detection liquid chip provided by the invention and a sequencing method is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.
Owner:SUREXAM BIO TECH

XRCC2 gene mutation detection specific primers and liquid chip

The invention discloses a XRCC2 gene mutation detection liquid chip, and specific primers. The XRCC2 gene mutation detection liquid chip mainly comprises: ASPE primers, wherein each ASPE primer is composed of 5'-terminal tag sequence, and 3'-terminal specific primer sequence targeting target gene mutation sites, and the specific primer sequence comprises SEQ ID No.9 and SEQ ID No.10 targeting G87A site, SEQ ID No.11 and SEQ ID No.12 targeting G158C site, SEQ ID No.13 and SEQ ID No.14 targeting A82G site, and / or SEQ ID No.15 and SEQ ID No.16 targeting A155C site; microballoons coated with anti-tag sequences; and amplification primers. Self-agreement ratio of detection results of the liquid chip with detection results of sequencing is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is realized.
Owner:SUREXAM BIO TECH

Liquid chip and specific primer for detecting SNP of GPIIIa gene and liquid chip and specific primer for detecting SNP of GPIIIa and COX-1 genes

The invention discloses a liquid chip and specific primer for detecting SNP of a GPIIIa gene. The liquid chip mainly comprises a wild type ASPE primer and a mutant type ASPE primer which are respectively designed aiming at the SNP loci of the GPIIIa gene, microspheres which are respectively coated with specific anti-tag sequences and have different colors of codes and an amplification primer for amplifying the GPIIIa gene target sequence containing the T196C SNP loci, wherein each ASPE primer is formed by the tag sequence at the 5, terminal and the specific primer, aiming at the SNP loci of the target gene, at the 3, terminal; and the anti-tag sequences are selected from SEQ ID NO.17 to SEQ ID NO.24 and correspondingly complement and are paired with the tag sequences selected from each ASPE primer. The invention also provides a liquid chip and specific primer for detecting SNP of COX-1 and GPIIIa genes. Besides the corresponding composition of the GPIIIa gene, the liquid chip also comprises ASPE primer pair aiming at the SNP loci of the COX-1 gene, microspheres coated with specific anti-tag sequences and an amplification primer. The liquid chip has very good signal to noise ratio,can avoid cross reaction and can realize parallel detection of a plurality of SNP loci.
Owner:SUREXAM BIO TECH

Specific primers and liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of MTHFR and FGF5 genes

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of MTHFR and FGF5 genes. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 9 and SEQ ID NO. 10 specific for a G121A SNP site, SEQ ID NO. 11 and SEQ ID NO. 12 specific for an A105G SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a G111A SNP site, and / or SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T196A SNP site of the FGF5 gene. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the MTHFR and FGF5 gene and the detection result of a sequencing method is up to 100%.
Owner:广州益善医学检验所有限公司

Specific primers and liquid phase chip for SNP (Single Nucleotide Polymorphism) detection of STK39 (Serine/Threonine Kinase) gene

The invention discloses specific primers and a liquid phase chip for SNP (Single Nucleotide Polymorphism) detection of a STK39 (Serine / Threonine Kinase) gene. The liquid phase chip mainly comprises an ASPE (Allele Specific Primer Extension) primer composed of a tag sequence at a 5' side and specific primers aiming at target gene mutation at a 3' side. The specific primers are respectively: SEQ ID NO.7 and SEQ ID NO.8 aiming at the T107C SNP site, SEQ ID NO.9 and SEQ ID NO.10 aiming at the G232A SNP site, and / or SEQ ID NO.11 and SEQ ID NO.12 aiming at the A190C SNP site; a microsphere coated with an anti-tag sequence; and an amplification primer. The coincidence rate of the detection result of the liquid phase chip for SNP detection of the STK39 gene provided by the invention and a sequencing method is as high as 100%. The prepared liquid phase chip has an excellent signal-noise ratio and can realize parallel detection of multiple polymorphism sites.
Owner:SUREXAM BIO TECH

CYP2B6 gene polymorphism detection specific primers and liquid chip

The invention discloses CYP2B6 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of 5' terminal tag sequence and 3' terminal specific primer sequences aiming at target gene mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.13 and SEQ ID NO.14 aiming at C123T site; SEQ ID NO.15 and SEQ ID NO.16 aiming at A101G site; SEQ ID NO.17 and SEQ ID NO.18 aiming at G118T site; SEQ ID NO.19 and SEQ ID NO.20 aiming at A77G site; SEQ ID NO.21 and SEQ IDNO.22 aiming at T139C site; and / or SEQ ID NO.23 and SEQ ID NO.24 aiming at C288T site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.
Owner:SUREXAM BIO TECH

IL-23R gene polymorphism detection specific primer and liquid phase chip

The invention discloses an interleukin-23 receptor (IL-23R) gene polymorphism detection specific primer and an IL-23R gene polymorphism detection liquid phase chip. The liquid phase chip mainly comprises an ASPE primer, microspheres and an amplified primer, wherein the ASPE primer consists of a tag sequence of a 5' end and specific primers of a 3' end, and the specific primers are selected from more than one pair of SEQ ID NO.9 and SEQ ID NO.10 aiming at C214T, SEQ ID NO.11 and SEQ ID NO.12 aiming at C235T, SEQ ID NO.13 and SEQ ID NO.14 aiming at T82G, and SEQ ID NO.15 and SEQ ID NO.16 aimingat C53T. The IL-23R gene polymorphism detection liquid phase chip has very good signal-noise ratio, the designed probe and an anti-tag sequence basically have no cross reaction, and the selection of a tag sequence and the anti-tag sequence and the combination of the tag sequence and the specific ASPE primer can avoid cross reaction and realize parallel detection of multiple polymorphic sites.
Owner:SUREXAM BIO TECH

Specific primer and liquid-phase chip for SNP (single nucleotide polymorphism) detection of PIGU (phosphatidylinositol glycan anchor biosynthesis, class U) genes

The invention discloses a liquid-phase chip for SNP (single nucleotide polymorphism) detection of PIGU (phosphatidylinositol glycan anchor biosynthesis, class U) genes, which mainly comprises an ASPE (allele specific primer extension) primer, a microsphere and an amplification primer, wherein the ASPE primer is composed of a tag sequence at a 5' terminal and specific primer sequences aiming at the mutant sites of target genes at a 3' terminal; the specific primer sequences comprise one of wild-type SEQ ID NO.1, SEQ ID NO.18 and SEQ ID NO.19 and one of mutant-type SEQ ID NO.2, SEQ ID NO.20 and SEQ ID NO.21; and the microsphere is coated by an anti-tag sequence. The coinciding rate of the detection result of the liquid-phase chip provided by the invention and the detection result of a sequencing method reaches 100%, and wild-type and mutant-type parallel detection of SNP sites is realized.
Owner:SUREXAM BIO TECH

AKT3 gene mutation detection specificity primer and liquid chip thereof

The invention discloses an AKT3 gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and a 3'-terminal specificity primer sequence focused on a target gene mutation site, wherein the specificity primer sequence comprises SEQ ID NO.7 and SEQ ID NO.8 focused on a G171R site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.
Owner:SUREXAM BIO TECH

Excision repair cross complementation group 2 (ERCC2) gene mutation detection specific primers and liquid chip kit

The invention discloses an excision repair cross complementation group 2 (ERCC2) gene mutation detection liquid chip and specific primers. The liquid chip mainly comprises ASPE primers and each one of the primers comprises a tag sequence at the end 5' and a specific primer sequence at the end 3' aiming at a target gene mutation site. The specific primer sequence comprises sequences shown in the formulas of SEQ ID NO. 9 and SEQ ID NO. 10 aiming at the G934A site, sequences shown in the formulas of SEQ ID NO. 11 and SEQ ID NO. 12 aiming at the T128G site, sequences shown in the formulas of SEQ ID NO. 13 and SEQ ID NO. 14 aiming at the C2133T site, and sequences shown in the formulas of SEQ ID NO. 15 and SEQ ID NO. 16 aiming at the A396C site. The liquid chip also comprises anti-tag sequence-coated microspheres and amplification primers. The detection result of the detection liquid chip and the detection result of the sequencing method have a coincidence rate of 100%. The ERCC2 gene mutation detection liquid chip realizes individual or parallel detection of multiple wild type and mutant type sites.
Owner:SUREXAM BIO TECH

TGM5 gene mutation detection specific primer and liquid phase chip

The invention discloses a TGM5 gene mutation detection liquid phase chip and a specific primer. The liquid phase chip mainly comprises: an ASPE primer composed of a tag sequence at 5'-terminal and specific primer sequences against the mutation site of a target gene at 3'-terminal, wherein the specific primer sequences comprise SEQ ID NO.7 and SEQ ID NO.8 against a T116C site, SEQ ID NO.9 and SEQ ID NO.10 against an A212G site, and / or SEQ ID NO.11 and SEQ ID NO.12 against an A117G site; a microsphere coated with an anti-tag sequence; and an amplimer. The coincidence rate between the detection result of the detection liquid phase chip and a sequencing method is 100%, and the wild and mutant parallel detection of a plurality of mutation sites can be realized.
Owner:SUREXAM BIO TECH

Specific primer and liquid chip for detecting polymorphism of SLCO1B3 gene

The invention discloses a specific primer and liquid chip for detecting polymorphism of an SLCO1B3 gene. The liquid chip mainly comprises ASPE (allele specific primer extension) primers, microspheres and amplification primers, wherein each ASPE primer is formed by tag sequences at the 5' terminal and specific primer sequences which are arranged at the 3' terminal and aim at the polymorphic site of the target gene; the specific primer sequences are SEQ ID NO.5 and SEQ ID NO.6 aiming at the T182G site and / or SEQ ID NO.7 and SEQ ID NO.8 aiming at the G77A site; and the microspheres are enveloped by anti-tag sequences. The detecting liquid chip provided by the invention has the advantages that the consistency of the detection result and the sequencing method is as high as 100%, thus parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.
Owner:SUREXAM BIO TECH

NPPA (natriuretic proatrial peptide A) gene SNP (Single Nucleotide Polymorphisms) detection specific primer and liquid phase chip

The invention discloses an NPPA (natriuretic proatrial peptide A) gene SNP (Single Nucleotide Polymorphisms) detection specific primer and a liquid phase chip. The liquid phase chip mainly comprises an ASPE primer, microballoon spheres and an amplimer; the ASPE primer comprises a tag sequence of a 5'end and a specific primer of a 3' end, wherein the specific primer aims at an SNP site; the specific primer aims at SEQ ID NO.7 and SEQ ID NO.8 of a rs5063SNP site, SEQ ID NO.9 and SEQ ID NO.10 of a rs5065SNP site and / or SEQ ID NO.11 and SEQ ID NO.12 of a rs5067SNP site; the tag sequence is selected from SEQ ID NO.1 to SEQ ID NO.6; and the microballoon spheres are respectively coated by specific anti-tag sequences and have different color codes. The result contact ratio of the liquid phase chip and a sequencing method is as high as 100 percent, and the prepared NPPA gene SNP detection liquid phase chip has very good signal-to-noise ratio.
Owner:SUREXAM BIO TECH

Specific detection primers and detection liquid phase chip for ADH1B gene mutation

The present invention discloses a detection liquid phase chip and specific primers for ADH1B gene mutation. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises A3170G site-targeted SEQ ID NO.13, A3170G site-targeted SEQ ID NO.14, G3641T site-targeted SEQ ID NO.15, G3641T site-targeted SEQ ID NO.16, G4564T site-targeted SEQ ID NO.17, G4564T site-targeted SEQ ID NO.18, A5998G site-targeted SEQ ID NO.19, A5998G site-targeted SEQ ID NO.20, G531A site-targeted SEQ ID NO.21, G531A site-targeted SEQ ID NO.22, and / or T4973C site-targeted SEQ ID NO.23 and T4973C site-targeted SEQ ID NO.24; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.
Owner:SUREXAM BIO TECH

MEK1 gene mutation detection specificity primer and liquid chip thereof

The invention discloses an MEK1 gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.9 and SEQ ID NO.10 focused on a Q56P site, SEQ ID NO.11 and SEQ ID NO.12 focused on a K57N site, SEQ ID NO.13 and SEQ ID NO.14 focused on a D67N site, and / or SEQ ID NO.15 and SEQ ID NO.16 focused on a P124L; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.
Owner:SUREXAM BIO TECH

LIG3 (DNA (deoxyribonucleic acid) Ligase III) gene mutation detection specific primers and liquid chip

The invention discloses an LIG3 (DNA (deoxyribonucleic acid) Ligase III) gene mutation detection liquid chip and specific primers. The liquid chip mainly comprises each ASPE (allele specific primer extension) primer formed by tag sequences at 5' terminal and specific primer sequences aiming at target gene mutation sites at 3' terminal, microspheres coated by anti-tag sequences as well as amplification primers, wherein the specific primer sequences are SEQ ID NO.11 and SEQ ID NO.12 aiming at C142T sites, SEQ ID NO.13 and SEQ ID NO.14 aiming at C214T sites, SEQ ID NO.15 and SEQ ID NO.16 aiming at A179G sites, SEQ ID NO.17 and SEQ ID NO.18 aiming at C158T sites and / or SEQ ID NO.19 and SEQ ID NO.20 aiming at G80A sites. The liquid chip has the advantages that the coincidence rate of the detection results of the detection liquid chip provided by the invention and a sequencing method is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.
Owner:SUREXAM BIO TECH

PDLIM5 gene mutation detection specific primer and liquid phase chip

The invention discloses a PDLIM5 gene mutation detection liquid phase chip and a specific primer. The liquid phase chip mainly comprises: an ASPE primer composed of a tag sequence at 5'-terminal and specific primer sequences against the mutation site of a target gene at 3'-terminal, wherein the specific primer sequences comprise SEQ ID NO.9 and SEQ ID NO.10 against a G76A site, SEQ ID NO.11 and SEQ ID NO.12 against a G277T site, SEQ ID NO.13 and SEQ ID NO.14 against a C100T site, and SEQ ID NO.15 and SEQ ID NO.16 against a T132C site; a microsphere coated with an anti-tag sequence; and an amplimer. The coincidence rate between the detection result of the detection liquid phase chip and a sequencing method is 100%, and the wild and mutant parallel detection of a plurality of mutation sites can be realized.
Owner:SUREXAM BIO TECH

CDKN1B gene mutation detection specific primers and liquid chip

The invention discloses a CDKN1B gene mutation detection liquid chip, and specific primers. The CDKN1B gene mutation detection liquid chip mainly comprises: ASPE primers, wherein each ASPE primer is composed of 5'-terminal tag sequence, and 3'-terminal specific primer sequence targeting target gene mutation sites, and the specific primer sequence comprises SEQ ID No.7 and SEQ ID No.8 targeting G-79A site, SEQ ID No.9 and SEQ ID No.10 targeting G-838T site, and / or SEQ ID No.11 and SEQ ID No.12 targeting T326G site; microballoons coated with anti-tag sequences; and amplification primers. Self-agreement ratio of detection results of the liquid chip with detection results of sequencing is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is realized.
Owner:SUREXAM BIO TECH

ABCG2 gene polymorphism detection specific primer and liquid phase chip

The invention discloses an ABCG2 gene detection specific primer and a liquid phase chip. The liquid phase chip main comprises each ASPE primer, a microsphere coated by anti-tag sequence and an amplification primer, wherein the ASPE primer comprises 5' end tap sequence and 3' end specific primer sequence aiming at gene mutation site; and the specific primer sequence comprises SEQ ID NO.7 and SEQ ID NO.8 aiming at the C184T site, SEQ ID NO.9 and SEQ ID NO.10 aiming at the C229A site, and / or SEQ ID NO.11 and SEQ ID NO.12 aiming at the C145T site. The consistency between the detection result of the liquid phase chip and that of a sequencing method reaches 100 percent, so that multi-mutational-site wild type and mutant type parallel detection is realized.
Owner:SUREXAM BIO TECH

Specific detection primers and detection liquid phase chip for SLC22A3 gene mutation

The present invention discloses a detection liquid phase chip and specific primers for SLC22A3 gene mutation. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises G161A site-targeted SEQ ID NO.13, G161A site-targeted SEQ ID NO.14, G191T site-targeted SEQ ID NO.15, G191T site-targeted SEQ ID NO.16, C150T site-targeted SEQ ID NO.17, C150T site-targeted SEQ ID NO.18, T182C site-targeted SEQ ID NO.19, T182C site-targeted SEQ ID NO.20, C91A site-targeted SEQ ID NO.21, C91A site-targeted SEQ ID NO.22, and / or C144T site-targeted SEQ ID NO.23 and C144T site-targeted SEQ ID NO.24; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.
Owner:SUREXAM BIO TECH

Specific primers and liquid phase chip for polymorphic detection of human hedgehog interacting protein (HHIP) gene

The invention discloses specific primers and a liquid phase chip for the polymorphic detection of a human hedgehog interacting protein (HHIP) gene. The liquid phase chip comprises allele specific primer extension (ASPE) primers consisting of tag sequences at the 5' end and specific primers at the 3' end, microspheres which are wrapped with specific anti-tag sequences and an amplification primer, wherein the sequences of the specific primers are selected from more than one pair of SEQ ID No. 5 and SEQ ID No. 6 aiming at T149C and SEQ ID No. 7 and SEQ ID No. 8 aiming at T61C. The liquid phase chip for the polymorphic detection of the HHIP gene has a high signal noise ratio, and cross reaction is not formed between a designed probe and the anti-tag sequences basically; and by the selection of the tag sequences and the anti-tag sequences and the combination of the tag sequences and the specific ASPE primers, the cross reaction can be prevented, and the parallel detection of multiple single nucleotide polymorphism (SNP) loci can be realized.
Owner:SUREXAM BIO TECH
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