The present invention discloses a detection
liquid phase chip and
specific primers for SLC22A3
gene mutation. The
liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal
target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises G161
A site-targeted SEQ ID NO.13, G161
A site-targeted SEQ ID NO.14, G191T site-targeted SEQ ID NO.15, G191T site-targeted SEQ ID NO.16, C150T site-targeted SEQ ID NO.17, C150T site-targeted SEQ ID NO.18, T182C site-targeted SEQ ID NO.19, T182C site-targeted SEQ ID NO.20, C91
A site-targeted SEQ ID NO.21, C91A site-targeted SEQ ID NO.22, and / or C144T site-targeted SEQ ID NO.23 and C144T site-targeted SEQ ID NO.24; anti-tag sequence coated microspheres; and amplification primers. According to the present invention,
coincidence frequency of the detection results of the detection
liquid phase chip and the sequencing method is up to 100%, and single and
parallel detection on the wild-type with multiple mutational sites and the
mutant-type with multiple mutational sites can be achieved.