133results about How to "Sensitive and specific recognition" patented technology

The invention discloses an HRAS gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20 and / or SEQ ID NO.21 focused on a Codon12 site, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24 and / or SEQ ID NO.25 focused on a Codon13 site, and / or SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29 and / or SEQ ID NO.30 focused on a Codon61 site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.

The invention discloses a specific primer and liquid chip for detecting polymorphism of a DPYD (dihydropyrimidine dehydrogenase) gene. The liquid chip mainly comprises ASPE (allele specific primer extension) primers, microspheres and amplification primers, wherein each ASPE primer is formed by tag sequences at the 5' terminal and specific primer sequences which are arranged at the 3' terminal and aim at the mutation site of the target gene; the specific primer sequences are SEQ ID NO.7 and SEQ ID NO.8 aiming at the G55A site, SEQ ID NO.9 and SEQ ID NO.10 aiming at the G141A site and / or SEQ ID NO.11 and SEQ ID NO.12 aiming at the G134T site; and the microspheres are enveloped by anti-tag sequences. The detecting liquid chip provided by the invention has the advantages that the consistency of the detection result and the sequencing method is as high as 100%, thus parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.

The invention discloses an FGFR1 gene mutation detection specific primer and a liquid chip. The liquid chip mainly comprises ASPE primers consisting of tag sequences at a 5' end and specific primer sequences aiming at target gene mutation sites at a 3' end, microspheres coated with anti-tag sequences and an amplification primer, wherein the specific primer sequences are as follows: SEQ ID NO.5 and SEQ ID NO.6 which aim at a C374T site, and / or SEQ ID NO.7 and SEQ ID NO.8 which aim at a C754A site. The detection liquid chip provided by the invention has the advantages that the matching rate between the detection result and a sequencing method is up to 100%, and the wild-type and mutant parallel detection of multiple mutant sites can be realized.

The invention discloses an RB1 gene mutation detection specific primer and a liquid chip. The liquid chip mainly comprises ASPE primers consisting of tag sequences at a 5' end and specific primer sequences aiming at target gene mutation sites at a 3' end, microspheres coated with anti-tag sequences and an amplification primer, wherein the specific primer sequences are as follows: SEQ ID NO.7 and SEQ ID NO.8 which aim at a C1654T site, SEQ ID NO.9 and SEQ ID NO.10 which aim at a C1666T site, and / or SEQ ID NO.11 and SEQ ID NO.12 which aim at a C1981T site. The detection liquid chip provided by the invention has the advantages that the matching rate between the detection result and a sequencing method is up to 100%, and the wild-type and mutant parallel detection of multiple mutant sites can be realized.

The invention discloses specific primers and a liquid-phase chip for the single nucleotide polymorphism (SNP) detection of the kit ligand gene (KITLG). The liquid-phase chip mainly comprises allele specific primers (ASPE), each of which consists of a tag sequence at a 5' end and specific primers aiming at mutational sites of target genes at a 3' end, microspheres wrapped by an anti-tag sequence and amplification primers, wherein sequences of the specific primers are one or more from the following pairs of sequences: SEQ ID No. 19 and SEQ ID No. 20, SEQ ID No. 21 and SEQ ID No. 22, SEQ ID No. 23 and SEQ ID No. 24, SEQ ID No. 25 and SEQ ID No. 26, SEQ ID No. 27 and SEQ ID No. 28, SEQ ID No. 29 and SEQ ID No. 30, SEQ ID No. 31 and SEQ ID No. 32, SEQ ID No. 33 and SEQ ID No. 34 and SEQ ID No.35 and SEQ ID No. 36. In the detection liquid-phase chip, the coincidence rate of detection results and a sequencing method is up to 100 percent, so the parallel detection of the wild type and mutanttype of polymorphic loci is realized.

The invention discloses a single-nucleotide polymorphism (SNP) detection specific primer of the CYP2C19 gene and a liquid phase chip. The liquid phase chip contains an ASPE primer composed of a tag sequence on the 5'-end and specific primers on the 3'-end which aims at the target genetic mutation, microspheres covered by the anti-tag sequence and an amplification primer, wherein the specific primers contain SEQ ID NO.25 and SEQ ID NO.26, SEQ ID NO.27 and SEQ ID NO.28 aiming at the C194T SNP site, SEQ ID NO.29 and SEQ ID NO.30, SEQ ID NO.31 and SEQ ID NO.32, SEQ ID NO.33 and SEQ ID NO.34, SEQID NO.35 and SEQ ID NO.36, SEQ ID NO.37 and SEQ ID NO.38, SEQ ID NO.39 and SEQ ID NO.40, SEQ ID NO.41 and SEQ ID NO.42, SEQ ID NO.43 and SEQ IDNO.44, SEQ ID NO.45 and SEQ ID NO.46, and / or SEQ ID NO.47 and SEQ ID NO.48. The coincidence rate of the detection result of the SNP detection liquid phase chip of the CYP2C 19 gene and the detection result adopting a sequencing method is up to 100%.

The present invention discloses a detection liquid phase chip and specific primers for HNF1B gene mutation. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises A12057G site-targeted SEQ ID NO.11, A12057G site-targeted SEQ ID NO.12, C8941T site-targeted SEQ ID NO.13, C8941T site-targeted SEQ ID NO.14, G35118A site-targeted SEQ ID NO.15, G35118A site-targeted SEQ ID NO.16, C3784A site-targeted SEQ ID NO.17, C3784A site-targeted SEQ IDNO.18, and / or G13582A site-targeted SEQ ID NO.19 and G13582A site-targeted SEQ ID NO.20; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.

The invention discloses a CDKN1A gene mutation detection liquid chip, and specific primers. The CDKN1A gene mutation detection liquid chip mainly comprises: ASPE primers, wherein each ASPE primer is composed of 5'-terminal tag sequence, and 3'-terminal specific primer sequence targeting target gene mutation sites, and the specific primer sequence comprises SEQ ID No.15 and SEQ ID No.16 targeting A7358T site, SEQ ID No.17 and SEQ ID No.18 targeting C98A site, SEQ ID No.19 and SEQ ID No.20 targeting G70A site, SEQ ID No.21 and SEQ ID No.22 targeting C258T site, SEQ ID No.23 and SEQ ID No.24 targeting T3745C site, SEQ ID No.25 and SEQ ID No.26 targeting G2763A site, and / or SEQ ID No.27 and SEQ ID No.28 targeting C4008T site; microballoons coated with different anti-tag sequences; and amplification primers. Self-agreement ratio of detection results of the liquid chip with detection results of sequencing is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is realized.

The invention discloses CYP1A2 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of 5' terminal tag sequence and 3' terminal specific primer sequences aiming at target gene mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.15 and SEQ ID NO.16 aiming at G216A site; SEQ IDNO.17 and SEQ ID NO.18 aiming at C96A site; SEQ ID NO.19 and SEQ ID NO.20 aiming at C139T site; SEQ ID NO.21 and SEQ ID NO.22 aiming at G100A T site; SEQ ID NO.23 and SEQ ID NO.24 aiming at A141T site; SEQ ID NO.25 and SEQ ID NO.26 aiming at C113T site; and / or SEQ ID NO.27 and SEQ ID NO.28 aiming at G110A site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.

The present invention discloses a detection liquid phase chip and specific primers for KLK3 gene mutation. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises G11453A site-targeted SEQ ID NO.11, G11453A site-targeted SEQ ID NO.12, G7670A site-targeted SEQ ID NO.13, G7670A site-targeted SEQ ID NO.14, A9367C site-targeted SEQ ID NO.15, A9367C site-targeted SEQ ID NO.16, T536C site-targeted SEQ ID NO.17, T536C site-targeted SEQ ID NO.18, and / or A15G site-targeted SEQ ID NO.19 and A15G site-targeted SEQ ID NO.20; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.

The invention discloses an AKT1 gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.5 and SEQ ID NO.6 focused on an E17K site, and / or SEQ ID NO.7 and SEQ ID NO.8 focused on an E49K site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.

The invention relates to a liquid phase chip for the polymorphic detection of an age-related maculopathy susceptibility 2 (ARMS2) gene and a high temperature factor A-1 (HTRA1) gene. The liquid phase chip comprises allele specific primer extension (ASPE) primers consisting of tag sequences at the 5' end and specific primers aiming at a mutant target gene at the 3' end, microspheres which are wrapped with anti-tag sequences and an amplification primer, wherein the specific primers are SEQ ID No. 10 and SEQ ID No. 11 for a G72T mutant locus of the ARMS2 gene, SEQ ID No. 12 to SEQ ID No. 14 for a C95G / A mutant locus of the HTRA1 gene, SEQ ID No. 15 and SEQ ID No. 16 for a G60A mutant locus of the HTRA1 gene and / or SEQ ID No. 17 and SEQ ID No. 18 for the insertion / deletion (I / D) mutation of the HTRA1 gene. The coincidence rate of a detection result of the liquid phase chip and a sequencing method is up to 100 percent, cross reaction can be prevented, and the parallel detection of multiplepolymorphic loci is realized.

The invention discloses CYP2A6 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of 5' terminal tag sequence and 3' terminal specific primer sequences aiming at target gene mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.17 and SEQ ID NO.18 aiming at G84T site; SEQ IDNO.19 and SEQ ID NO.20 aiming at G133A site; SEQ ID NO.21 and SEQ ID NO.22 aiming at T229A site; SEQ ID NO.23 and SEQ ID NO.24 aiming at T106C site; SEQ ID NO.25 and SEQ ID NO.26 aiming at G148T site; SEQ ID NO.27 and SEQ ID NO.28 aiming at T149G site; SEQ ID NO.29 and SEQ IDNO.30 aiming at T53C site; and / or SEQ ID NO.31 and SEQ ID NO.32 aiming at G108A site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.

The invention discloses a liquid chip and specific primer for detecting an SLC22A1 gene. The liquid chip mainly comprises ASPE (allele specific primer extension) primers, microspheres and amplification primers, wherein each ASPE primer is formed by tag sequences at the 5' terminal and specific primer sequences which are arranged at the 3' terminal and aim at the mutation site of the target gene; the specific primer sequences are SEQ ID NO.19 and SEQ ID NO.20 aiming at the C117T site, SEQ ID NO.21 and SEQ ID NO.22 aiming at the T198C site, SEQ ID NO.23 and SEQ ID NO.24 aiming at the C86T site, SEQ ID NO.25 and SEQ ID NO.26 aiming at the C97G site, SEQ ID NO.27 and SEQ ID NO.28 aiming at the C164T site, SEQ ID NO.29 and SEQ ID NO.30 aiming at the G123T site, SEQ ID NO.31 and SEQ ID NO.32 aiming at the G127A site, SEQ ID NO.33 and SEQ ID NO.34 aiming at the A148G site and / or SEQ ID NO.35 and SEQ ID NO.36 aiming at the G129C site; and the microspheres are enveloped by anti-tag sequences. The detecting liquid chip provided by the invention has the advantages that the consistency of the detection result and the sequencing method is as high as 100%, thus parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.

The invention relates to a liquid phase chip for the polymorphic detection of an age-related maculopathy susceptibility 2 (ARMS2) gene and a high temperature factor A-1 (HTRA1) gene. The liquid phase chip comprises allele specific primer extension (ASPE) primers consisting of tag sequences at the 5' end and specific primers aiming at a mutant target gene at the 3' end, microspheres which are wrapped with anti-tag sequences and an amplification primer, wherein the specific primers are SEQ ID No. 10 and SEQ ID No. 11 for a G72T mutant locus of the ARMS2 gene, SEQ ID No. 12 to SEQ ID No. 14 for a C95G / A mutant locus of the HTRA1 gene, SEQ ID No. 15 and SEQ ID No. 16 for a G60A mutant locus of the HTRA1 gene and / or SEQ ID No. 17 and SEQ ID No. 18 for the insertion / deletion (I / D) mutation of the HTRA1 gene. The coincidence rate of a detection result of the liquid phase chip and a sequencing method is up to 100 percent, cross reaction can be prevented, and the parallel detection of multiplepolymorphic loci is realized.