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Analysis device based on electrochemical luminescence technology and double-pole electrode configuration

A bipolar electrode and electrochemistry technology, applied in the field of electrochemistry, can solve problems such as changing the shape or state of samples, and achieve the effects of improving sensitivity and reliability, high economic benefits, and enhancing broad spectrum

Active Publication Date: 2016-08-17
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a detection process leads to high requirements on the precision and parallelism of the processing of the sample and its carrier, and may even change the original shape or state of the sample

Method used

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  • Analysis device based on electrochemical luminescence technology and double-pole electrode configuration
  • Analysis device based on electrochemical luminescence technology and double-pole electrode configuration
  • Analysis device based on electrochemical luminescence technology and double-pole electrode configuration

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Effect test

Embodiment 1

[0023] The reagents used in the method of the present invention include: 30% hydrogen peroxide solution, 0.01M phosphate buffer saline (PBS, pH 7.4), and the reaction reagent is ruthenium bipyridyl (Ru(bpy)32+) of 1.0mM and 5.0 mM tri-n-propylamine (TPA) mixed solution. The apparatus used consisted of an analytical set-up based on electrochemiluminescence technique and bipolar electrode configuration.

[0024] The device can realize rapid detection of hydrogen peroxide solutions with different concentrations in a bright environment.

[0025] The specific implementation process of this method is as follows:

[0026] Step 1: Preparation of series concentrations of hydrogen peroxide

[0027] The original concentration of hydrogen peroxide was diluted step by step with 0.01M PBS solution to 10-3M, 10-4M, 10-5M, 10-6M, 10-7M and 10-8M hydrogen peroxide solutions.

[0028] Step 2: Detection of ECL signals of different concentrations of hydrogen peroxide

[0029]Add 0.01M PBS sol...

Embodiment 2

[0031] The reagents used in the method of the present invention include: glucose, glucose oxidase solution, 0.01M phosphate buffer saline (PBS, pH7.4), and the reaction reagent is ruthenium bipyridyl (Ru(bpy)32+) of 1.0mM Mix the solution with 5.0 mM tri-n-propylamine (TPA). The apparatus used consisted of an analytical set-up based on electrochemiluminescence technique and bipolar electrode configuration.

[0032] Utilizing the device in a bright environment can realize in-situ rapid detection of glucose solutions with different concentrations.

[0033] The specific implementation process of this method is as follows:

[0034] Step 1: Preparation of serial concentrations of glucose

[0035] 10-3M, 10-4M, 10-5M, 10-6M, 10-7M and 10-8M glucose solutions were prepared with 0.01M PBS as solvent.

[0036] Step 2: Detection of ECL signals in different concentrations of glucose solutions

[0037] Add 2.0 mL of 0.01M PBS solution to the reservoir, and add 20 L of glucose oxidase ...

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Abstract

The invention discloses an analysis device based on an electrochemical luminescence technology and double-pole electrode configuration. The analysis device comprises a mini-type dark room for storing a reaction reagent and having a reaction and a liquid storage tank for storing detection objects and having an oxidization-reduction reaction, wherein a double-pole electrode and an electric signal applying unit are arranged between the mini-type dark room and the liquid storage tank respectively; one end of the double-pole electrode is packaged in an inner cavity at the bottom of the mini-type dark room and the other end of the double-pole electrode stretches into the liquid storage tank; and meanwhile, a positive pole of the electric signal applying unit stretches into the liquid storage tank through a driving electrode and a negative pole of the electric signal applying unit is connected with the top of the mini-type dark room through a driving electrode. According to the analysis device based on the electrochemical luminescence technology and the double-pole electrode configuration, the stored detection objects are linked with signal molecules, so that the oxidization-reduction reaction in the liquid storage tank is transmitted into the mini-type dark room in an electron form, a light signal is generated and a corresponding electrochemical luminescence signal is realized, so that the sensitivity and reliability of detection are extremely improved.

Description

technical field [0001] The invention relates to the field of electrochemical technology, in particular to an analysis device based on electrochemiluminescence technology and bipolar electrode structure. Background technique [0002] Electrochemiluminescence is an analytical method based on electrochemistry that uses the light signal of electrical excitation as the measurement. It refers to the luminescence phenomenon that occurs when the reactant reacts on the surface of the electrode to generate an excited state, and when it returns to the ground state. On the basis of inheriting the advantages of high electrochemical sensitivity and good controllability, it reduces the influence of electrochemical background signals, thereby further improving the detection sensitivity. By controlling the conditions, the detection results can be presented visually, which broadens the scope of analysis and detection, and has been successfully used in the detection of various metal ions, smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
CPCG01N21/76G01N27/305
Inventor 潘建斌施海蔚徐静娟陈洪渊
Owner NANJING UNIV
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