Visual LAMP detection kit for streptococcic mastitis pathogenic bacteria

Abstract

The invention discloses a visual LAMP (Loop-Mediated Isothermal Amplification) detection kit for streptococcic mastitis pathogenic bacteria, wherein the inventor designs two specific inner primers and two specific outer primers according to the common gene conservative area of the streptococcic mastitis pathogenic bacteria by virtue of adopting the loop-mediated isothermal amplification technique, so that the visual LAMP detection kit is obtained. A method for detecting the streptococcic mastitis pathogenic bacteria by using the LAMP detection kit only needs a common water bath kettle in stead of expensive PCR (Polymerase Chain Reaction) instrument, and the method is simple to operate and low in cost, but higher in sensitivity than PCR detection; the detection results are visual and can be obtained through judgment by naked eyes, and the results do not need to be observed through gel electrophoresis ultraviolet analysis and development. The visual LAMP detection kit can be used for overcoming the defects of long time taken, high workload, easy cross contamination, complex operation and the like of the existing detection technology, and is more specific and sensitive than the conventional detection method, and thus applicable to quick detection in basic-level veterinary stations and dairy farms; besides, the visual LAMP detection kit has excellent application prospect.

Description

technical field [0001] The invention belongs to the field of detection of streptococcal mastitis pathogenic bacteria, in particular to a visual LAMP detection kit for streptococcal mastitis pathogenic bacteria. Background technique [0002] Mastitis is one of the most common diseases in dairy farms and causes the most serious economic losses. It restricts the healthy development of dairy farming and dairy processing industries, and endangers public health and human health. How to effectively prevent and control this disease has become an important issue at home and abroad. focus of attention. Staphylococcus aureus, Streptococcus, and Escherichia coli, the incidence of these three pathogenic bacteria accounted for more than 90% of the total incidence of mastitis in dairy cows. Common Streptococcus pathogenic bacteria (Streptococcus) of mastitis in dairy cows mainly include Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis, among which Streptococcu...

AI Technical Summary

Problems solved by technology

Most of the currently established LAMP reaction methods use agarose gel electrophoresis, ultraviolet analysis and imaging, or add fluorescent dyes after the reaction is completed to determine the results. They can only analyze the final results of the LAMP reaction, and there is a risk of aerosol contamination of the laboratory. Due to the lack of real-time monitoring of the reaction, it is difficult to rule out these interference factors, so it is impossible to make an accurate judgment on the test results

[0005] At present, there are no relevant reports on the establishment of a visual detection method for LAMP, the pathogenic bacteria of streptococcal mastitis, at home and abroad.

Images