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229 results about "Gene expansion" patented technology
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Methods for identifying DNA copy number changes
InactiveUS20050064476A1Reduce complexityMicrobiological testing/measurementProteomicsGenomic DNANormal tissue
Methods of identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of a plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that are predicted to be present in a reduced complexity sample. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.
Owner:AFFYMETRIX INC
Methods for identifying DNA copy number changes
Methods of identifying allele-specific changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of a plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that are predicted to be present in a reduced complexity sample. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.
Owner:AFFYMETRIX INC
Selective gene amplification
InactiveUS20050003380A1Inhibit migrationMicrobiological testing/measurementFermentationGene productGene
A method is provided for selecting nucleric acids encoding gene product is in which the nucleic acid encoding the gene product is selectly and quantitatively amplified depending on the activity of the gene itself or the gene product. The method may, for example, be used in selecting gene products arising from in vitro evolution of molecular libraries.
Owner:MEDICAL RESEARCH COUNCIL
Gene amplifying method
InactiveUS6261846B1Efficient amplificationEasy to detectSugar derivativesMicrobiological testing/measurementDouble strandEnzyme
A gene amplifying method for efficiently amplifying a gene without using enzyme or branched DNA. A plurality of pairs of probes each composed of three or more portions complementary to such portions in the other probe are used, and hybridized such that they cross in alternation to form a double-stranded polymer.
Owner:SEKISUI MEDICAL CO LTD
Cancer gene mutation and gene amplification detection
ActiveCN104630375AAmplified equalizationImprove efficiencyMicrobiological testing/measurementDNA/RNA fragmentationMultiplexMedicine
The invention provides a primer set for simultaneously detecting mutation of a plurality of regions of two or more exons of one or more cancer drive genes in a sample on the basis of multiplex PCR (polymerase chain reaction) and high-flux sequencing techniques, a kit comprising the primer set, and a method for amplifying the cancer drive gene or detecting the cancer drive gene by using the primer set and / or kit. The mutation detection result can be used for instructing cancer clinic medication, auxiliary diagnosis or prognosis judgment on certain cancers.
Owner:北京圣谷智汇医学检验所有限公司
Method for detecting multiple endocrine adenoma II gene mutation
InactiveCN101148684AMicrobiological testing/measurementBiological testingRet geneMultiple endocrine adenomas
The present invention belongs to the field of biotechnology, and discloses one method of in vitro determining whether to have RET gene variation in the nucleic acid sample, one kit for determining RET gene variation and one process of obtaining RET gene amplifying product. The method of the present invention is accurate, simple, fast and stable.
Owner:RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Single-tube amplification kit for simultaneously detecting alpha and beta thalassemia genes
ActiveCN104212910AShort detection timeLow costMicrobiological testing/measurementBeta thalassemiaMutant
The invention discloses a single-tube amplification kit for simultaneously detecting alpha and beta thalassemia genes, and the kit comprises (1) a gene chip on which a probe is arranged as SEQID No:1-37 and its complementary sequence; (2) one group of primers which is used for multiple PCR (polymerase chain reaction) and regarded as SEDID No. 38-46. Through the kit, alpha and beta thalassemia genes can be simultaneously amplified in the same reaction tube, and three deleted alpha-thalassemia genes (SEA, -alpha 3.7 and -alpha 4.2), three mutant alpha-thalassemia genes (CS, QS and WS) and 19 mutant beta-thalassemia genes can be simultaneously detected. In the same reaction tube, alpha-thalassemia genes and beta-thalassemia genes can be also simultaneously amplified, thereby greatly improving the diagnostic efficiency and accuracy, reducing the cost and shortening he detection time, thus the kit has the great significance of thalassemia crowd screening, genetic counseling and antenatal diagnosis.
Owner:GUANGDONG HYBRIBIO BIOTECH CO LTD +2
Detection primer combination and kit for HER2 (human epidermal growth factor receptor 2) gene amplification
InactiveCN105112522AQuick checkEasy to detectMicrobiological testing/measurementDNA/RNA fragmentationFluoProbesTherapeutic effect
The invention discloses a detection primer combination and a kit for HER2 (human epidermal growth factor receptor 2) gene amplification. The detection primer combination comprises an HER2 primer combination and a GAPDH reference primer combination; each combination comprises upstream and downstream amplification primers of a target gene of the combination and a Taqman fluorescent probe. The kit comprises the detection primer combination, a positive quality control and a negative quality control. By using the detection primer combination and the kit, whether the HER2 gene in patients with breast carcinoma is amplified or not can be detected quickly and simply; compared with the FISH (fluorescent in situ hybridization) method, a method using the detection primer combination and the kit is high in detection sensitivity, low time consumption, generally 1.5 to 2 hours for acquiring reaction results, low in cost, low in false positive ratio, and suitable for large-scale clinical development; thus, quick, effective and accurate detection is achieved for HER2 gene amplification, and timely disease treatment and treatment effect monitoring are guaranteed.
Owner:北京鑫诺美迪基因检测技术有限公司
Quantitative detection method for gene copy number
InactiveCN102618646ASync detectionHigh sensitivityMicrobiological testing/measurementReference genesReference sample
The invention discloses a quantitative detection method for a gene copy number. The method comprises the following steps of: respectively designing primers and fluorescence probes for target gene and reference gene amplification sequences, and designing a closed probe for the target gene amplification sequence; detecting a sample: performing multiple TaqMan fluorescent quantitative polymerase chain reaction (PCR) on target genes and reference genes by using the DNA sample to be detected as a template, and respectively recording Ct values by using a DNA sample with known target gene copy number as a reference sample; and analyzing the result. By the method, the copy number of the functional genes can be directly and quantitatively detected, and deletion, deletion number and multiple copies (CNVs) of the detected genes are directly reflected, so that synchronous quick detection of gene deletion and multiple gene copies is realized; and the method is high in sensitivity, the synchronous quick detection of the gene deletion and the multiple gene copies can be finished only by a common fluorescent quantitative PCR instrument, and experiments prove that the method is accurate and reliable.
Owner:SOUTHERN MEDICAL UNIVERSITY
Recombinant Taq DNA polymerase and preparation method thereof
ActiveCN103602643AImprove expression efficiencyImprove purification efficiencyTransferasesMicroorganism based processesActive proteinGenetic engineering
The invention relates to the technical field of genetic engineering, and particularly relates to a method for preparing a gene recombinant expression enzyme of Thermusaquaticus TaqDNA polymerase. The nucleotide sequence of a gene and the amino acid sequence of an encoded protein are respectively shown as SEQIDNO:1 and SEQIDNO:2. According to the invention, the method comprises the following steps: cloning a gene sequence by using a gene cloning technique, and building a prokaryotic expression vector; by using a recombinant strain of a built TaqDNA polymerase gene, carrying out suspension by using an LB culture solution; after carrying out culturing for 4.5 hours at a temperature of 37 DEG C, adding IPTG (isopropyl-beta-d-thiogalactoside) with a final concentration of 0.5 mM into the obtained substance to induce for 16-20 hours; after thalli are collected in a centrifugal mode, dissociating the thalli by using 2-4 mg / ml lysozyme, so that protein fluid is obtained; and filtering the protein fluid by using a chromatographic column and a dialysis bag so as to obtain high-purity active protein TaqDNA polymerase. Compared with the prior art, stability of a production process is good, activity of obtained TaqDNA polymerase is high, the yield and purity of products are effectively ensured, and amplification effect when being applied to a gene PCR (polymerase chain reaction) is good.
Owner:哲尔基因科技(上海)有限公司
Single tube in situ nested polymerase chain reaction method and its use
InactiveCN1858219AEasy to operateOperation specificMicrobiological testing/measurementFermentationAgricultural scienceReaction tube
The present invention relates to single tube in-situ nested PCR method and its application in nucleotide detection. The reaction liquid contains both inside primer and outer primer, and the inner primer has mismatching with target gene sequence or one section of non-specific gene sequence in the 5' end. The inner primer and the original template have highest annealing temperature lower than that of the first round reaction, while the inner primer and the matched template have the melting temperature higher than the highest allowed annealing temperature of the outer primer. The first round PCR cycle annealing temperature is controlled for only the outer primer to be capable of annealing amplification, and the second round PCR cycle annealing temperature is raised for only the inner primer to be capable of annealing amplification. Between the first round and the second round, there are two or more transition stages with lower circular annealing temperature for the inner primer and the original template to form matching template to start the second round reaction.
Owner:徐定邦 +1
Genetically expanded cell free protein synthesis systems, methods and kits
InactiveUS20170292139A1Convenience to mergeImprove translationLigasesFermentationEscherichia coliBiological body
This invention relates to methods of producing a rare amino acid- or non-natural amino acid-containing protein in a cell free protein synthesis system and kits for use in and for accomplishing same. Specifically, the methods comprise the steps of expressing at least one orthogonal suppressor tRNA (o-tRNA) / aminoacyl-tRNA synthetase (aaRS) pair or derivatives thereof specific for incorporation of a rare amino acid- or non-natural amino acid in an E. coli organism; preparing a lysate of said E. coli organism expressing said orthogonal suppressor tRNA (o-tRNA) / aminoacyl-tRNA synthetase (aaRS) pair; and contacting said lysate with a template DNA containing a mutant gene in which at least one amino acid codon at a given site of the protein-encoding gene has been mutated into an amber or ochre mutation and further providing a cognate rare amino acid or non-natural amino acid and other factors necessary for protein synthesis; wherein protein synthesis occurs following said contact to produce a protein containing said at least one rare amino acid or said non-natural amino acid. Kits for use are described, as well.
Owner:B G NEGEV TECH & APPL LTD
Detection chip for tumor driving gene and application thereof
The invention discloses a detection chip for a tumor driving gene and application thereof. The detection chip for the tumor driving gene comprises an ALK (anaplastic lymphoma kinase) fusion detection agent, an EGFR (epidermal growth factor receptor) fusion detection agent, an RET (reticulocyte) fusion detection agent and an ROS1 (reactive oxygen species 1) fusion detection agent. Proofed by the results of clinical detect, after fusion probes corresponding to the ALK, the EGFR, the RET and the ROS1 are specifically designed, the detection chip has the advantages that the sensitivity is high, and the gene fusion between a particular site area of the ALK, the EGFR, the RET and the ROS1 and fusion segments is specifically detected. The invention further discloses a detection chip for detecting a second sequence group of the gene mutation and a third sequence group of the gene amplification; after detecting once, the gene fusion, gene mutation and gene amplification of the multiple tumor driving genes, such as the ALK, BRAF (aserine / theroninespecific kinases), DDR2 (discordin domain receptor 2), the EGFR, ERBB2 (receptor tyrosine kinase 2), FGFR1 (fibroblast growth factor receptor 1), KRAS (kirstenrat sarcoma viral oncogene), MET (methionine), NRAS, PIK3CA (phosphatidylino-sitol 3-kinases), the RET and the ROS1.
Owner:常州桐树生物科技有限公司
Compositions for improving gene amplification
InactiveUS20120028259A1Improve polymerase performanceImprove performanceMicrobiological testing/measurementFermentationBlood componentGC-content
The present invention generally relates to amplfication reactions. One aspect of the invention provides amplification reaction enhancer compositions comprising trehalose, carnitine, and a non-ionic detergent, such as NP40. These enhancer compositions can improve efficiency, specificity, and sensitivity of amplification reactions in conventional and real-time PCR and RT-PCR. In addition, these compositions permit nucleic acid amplification directly in crude samples containing blood, blood components, or soil extract with little or no nucleic acid extraction prior to amplification. Another aspect of the invention provides a method of enhancing an amplification reaction containing a crude blood sample with heparin. Another improvement derived from the invention is improved detection of difficult, high GC content nucleic acid targets.
Owner:DNA POLYMERASE TECH
Nucleotide sequence and kit for HER-2 gene copy number amplification detection, and application of kit
ActiveCN107739760AThe result is accurateLow costMicrobiological testing/measurementDNA/RNA fragmentationMolecular diagnostic techniquesNucleic acid sequencing
The invention discloses a nucleotide sequence and a kit for detecting a human epidermal growth factor receptor-2 (HER-2), which belong to the technical field of molecular diagnosis. A primer and a probe for detecting the HER-2 provided by the invention can be used for specifically amplifying and detecting amplification of an HER-2 gene. The invention also discloses the detection kit for detectingthe HER-2 gene based on a microdroplet type PCR (Polymerase Chain Reaction) system, so that the amplification of the HER-2 gene can be quickly, sensitively and accurately detected. The nucleotide sequence and the kit provided by the invention have the advantages of simplicity and convenience in operation, high specificity, high sensibility, low cost, high throughput and the like, can be used for quickly detecting clinic HER-2 gene amplification, and provide references for diagnosis and treatment of the clinic human epidermal growth factor receptor-2.
Owner:昆山迪安医学检验实验室有限公司
Method for preparing internal standard of molecular weight, and internal standard of molecular weight prepared by using the method
ActiveCN101050474ASimple and fast operationShorten the timeMicrobiological testing/measurementFermentationFluorescenceInternal standard
This invention relates to a method for preparing molecular weight internal standard. The method comprises: immobilizing upstream primer 5'GCGAAACCCGACAGGACTA3' with pUC18 plasmid as the template, designing downstream primers with a certain distance, and proliferating to obtain multiple nucleotide segments. The method obtains a series of segments with different lengths by immobilizing the upstream primer and designing the downstream primers in sequence. The traditional method for synthesizing primers by fluorescent labeling has such disadvantages as high cost and short preservation time. The method in this invention synthesizes the upstream immobilized primer only, which can largely lower the primer synthesis cost and production cost. Since the upstream primer is the same, the lengths and annealing temperatures of the downstream primers are similar, and the proliferation conditions for all segments are identical, which can facilitate easy operation (all proliferations are performed on the same gene proliferation apparatus with the same reaction program), saved time, high working efficiency, and large-scale production.
Owner:DINGSHENG TECH BEIJING +1
Primer set for amplification of CYP2C19 gene, reagent for amplification of CYP2C19 gene comprising the same, and use of the same
InactiveCN101501223AReduce effortLow costMicrobiological testing/measurementRecombinant DNA-technologyForward primerNucleotide sequencing
Disclosed is a primer set for amplifying a target region in CYP2C19 gene which contains a site to be detected by a gene amplification method, and which enables to amplify the region specifically. Two pairs of primer sets are used, each of which comprises a forward primer comprising a nucleotide sequence depicted in each of SEQ ID NOs:12 and 32 and a reverse primer comprising a nucleotide sequence depicted in each of SEQ ID NOs:22 and 48, respectively. These primer sets enable to amplify two regions each containing a site having each of two types of polymorphisms occurring in CYP2C19 gene (CYP2C19 gene*2, CYP2C19*3) simultaneously in a single reaction solution.
Owner:ARKRAY INC
Goose-origin gene RIG-I (retinoic acid-inducible gene-I) with anti-Newcastle disease virus activities and application thereof
InactiveCN103173455AGrowth inhibitionCan exert anti-NDV effectFungiBacteriaEmbryoMessenger ribonucleic acid
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
Method for rapidly and efficiently screening rhamnolipid producing bacteria nutrition system
InactiveCN104498526AReduce lossReduce workloadMicrobiological testing/measurementMicroorganism based processesNutritionGenetic engineering
The invention relates to a method for rapidly and efficiently screening a rhamnolipid producing bacteria nutrition system. The method comprises the following steps: synthesizing a primer of rhamnolipid producing gene rhlAB, performing gene amplification by using PCR, synthesizing rhlAB gene fragments, connecting the rhlAB gene fragment to the position in front of lux luminescence gene on pMS402 plasmid by utilizing gene engineering shearing and splicing technology, then transferring the recombinant plasmid into pseudomonas aeruginosa DN1, coating the recombinant strain onto an LB solid plate containing 200mg / L-300mg / LTmp, screening out positive clone, naming the strain as rhlAB-lux strain, picking out positive monoclonal colonies to be enriched on the solid LB culture medium, refrigeratingat at 4DEG C for later use; taking out the refrigerated rhlAB-lux strain, and enriching the thallus overnight; and screening a nutrient optimized system. According to the method, the luminescence value can be determined by a multifunctional microplate reader, and the method has the characteristics that the flux is high, and the screening process is simplified and rapid.
Owner:NORTHWEST UNIV(CN)
Establishment and application of novel HIV-1 drug resistance detection method
ActiveCN108866207AAvoid amplificationThe problem of low amplification efficiency is avoidedMicrobiological testing/measurementDNA/RNA fragmentationPublic healthDrug resistance
The invention discloses establishment and application of a novel HIV-1 drug resistance detection method. The novel HIV-1 drug resistance detection method established according to the invention has theadvantages that 1, by utilizing multiple sets of primer combinations, a problem of low amplification efficiency of target genes, which is caused by differences of different subtypes of strain sequences, is considered; and 2, synthesis of PR, RT and IN target gene cDNA and first-round amplification of a target gene nested PCR are completed in one reaction tube so as to avoid respective amplification on the target genes, simplify the experimental process and save reagent cost by 50%. The detection method established according to the invention can simultaneously cover multiple strain subtypes, and simultaneously cover multiple gene regions, which enables HIV-1 drug resistance detection efficiency to be greatly improved, and has the great influence on aids prevention and public health.
Owner:ACADEMY OF MILITARY MEDICAL SCI
Primer set for amplifying cyp2c19 gene, reagent for amplifying cyp2c19 gene containing the same, and the uses thereof
InactiveUS20090269756A1Improve efficiencyEnlarge regionSugar derivativesMicrobiological testing/measurementForward primerGene expansion
Primer sets for amplifying target regions containing sites to be detected in the CYP2C19 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Two pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 12 and 32 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 22 and 48, respectively. The use of these primer sets makes it possible to amplify two target regions including parts where two types of polymorphisms (CYP2C19*2 and CYP2C19*3) of the CYP2C19 gene are generated, respectively, in the same reaction solution at the same time.
Owner:ARKRAY INC
Detection method of yak FOXO1 gene single nucleotide polymorphism and kit thereof
ActiveCN105441567APrecise positioningSensitive positioningMicrobiological testing/measurementNucleotideA-DNA
The invention relates to the technical field of gene polymorphism detection, in particular to a detection method of yak FOXO1 gene single nucleotide polymorphism and a kit thereof. The detection method comprises the following steps: 1) preparing a yak FOXO1 gene amplification product; 2) synthesizing a high and low temperature interior label; 3) preparing an HRM-PCR (High Resolution Melt-Polymerase Chain Reaction) amplification product; 4) collecting a fluorescence signal. Compared with an HRM method used for detecting polymorphic sites in the prior art, a gene probe designed by the detection method is more accurate and flexible in positioning, is accurate in parting, does not need PCR post-processing, has high working efficiency and good repeatability and is low in sample quality and quantity requirements, especially, the concentration requirement of a DNA template can be lowered to 0.1ng / mu L, and time and labor are saved when a sample size is large. Meanwhile, the detection kit has the advantages of high sensitiveness, high detection speed and good stability.
Owner:GANSU AGRI UNIV
Primer group for detecting Bordetella pertussis, detection test kit and detection method
InactiveCN101875974AReduced Pollution ChancesIncrease costMicrobiological testing/measurementDNA/RNA fragmentationNucleotide sequencingGel electrophoresis
The invention discloses a primer group for detecting Bordetella pertussis, a detection test kit and a detection method. The primer group comprises an IS481 gene amplification pair and / or a PT gene amplification primer pair, wherein the primer pair sequence of the IS481 gene amplification pair is a nucleotide sequence shown as SEQ IDNO:1 and SEQ IDNO:2, and the primer pair sequence of the PT gene amplification primer pair is a nucleotide sequence shown as SEQ IDNO:3 and SEQ IDNO:4. The invention detects two genes simultaneously in the same reaction system, and the two genes can be detected through gel electrophoresis. The specificity of detection is guaranteed, and the sensitivity of detection is increased; meanwhile, detection efficiency is greatly increased, and detection cost is saved.
Owner:SHENZHEN CHILDRENS HOSPITAL
Amplification method of porcine epidemic diarrhea virus S-gene epitope
ActiveCN103088039AMicroorganism based processesFermentationSequence analysisPorcine epidemic diarrhoea virus
The invention discloses an amplification method of porcine epidemic diarrhea virus S-gene epitope. The amplification method comprises the steps of: (1) extraction of virus RNA (Ribonucleic Acid); (2) amplification of RT-PCR (Reverse Transcription-Polymerase Chain Reaction); (3) amplification of nested PCR (Reverse Transcription-Polymerase); and (4) cloning, sequencing analysis and the like of a PCR product and the like. An S-gene of the porcine epidemic diarrhea virus (PEDV) is amplified, and the amplified fragment is 782bp in length (situated in a range from 1316bp-2097bp of the S-gene) and comprises neutralized antigenic epitope situated in a range from 1495bp-1914bp of the S-gene; and according to the amplification method, different generations of S-genes of PEDV (porcine epidemic diarrhea virus) seed, which are cultured on a vero cell, can be amplified, and the S gene of the porcine epidemic diarrhea virus in a cell culture and passage process, particularly the sequence change of the neutralized antigenic epitope, is captured, thus the relationship between the sequence change of the neutralized antigenic epitope and the immune efficacy change of the PEDV can be known.
Owner:GUANGDONG WENS DAHUANONG BIOTECH +1
Primer Set for Amplification of MTHFR Gene, MTHFR Gene Amplification Reagent Containing the Same, and Use of the Same
The present invention provides a primer set for specifically amplifying a target region in a MTHFR gene by a nucleic acid amplification method, a MTHFR gene amplification reagent containing the primer set, and use of the primer set.
Owner:ARKRAY INC
Adenocarcinoma specificity EpCAM-GM-CSF genetic recombinant fusion protein and preparation method thereof
ActiveCN104774270APeptide/protein ingredientsAntibody medical ingredientsEscherichia coliAdenocarcinoma
The invention relates to adenocarcinoma specificity EpCAM-GM-CSF genetic recombinant fusion protein and a preparation method thereof. The method comprises the first step of designing and compositing a primer, the second step of amplifying and sequencing genes, the third step of constructing a recombinant vector pET-EpCAM-hGM-CSF, the fourth step of screening and identifying the recombinant vector pET-EpCAM-hGM-CSF, the fifth step of recombining plasmid pET-EpCAM-hGM-CSF to transform escherichia coli Rosetta, the sixth step of recombining EpCAM-hGM-CSF fusion protein to induce to express in escherichia coli Rosetta, the seventh step of purifying the EpCAM-hGM-CSF fusion protein, and the eighth step of permeating and concentrating the purified EpCAM-hGM-CSF fusion protein.
Owner:浓孚雨医药河北有限公司
Gene synthesizing method of luteolin-producing yeast strain as well as strain and application
PendingCN108315343ALow costIncrease productionFungiMicroorganism based processesBiotechnologyComplementary deoxyribonucleic acid
The invention discloses a gene synthesizing method of a luteolin-producing yeast strain as well as the strain and application. The gene synthesizing method is characterized in that genes PAL, C4H, 4CL, CHS, CHI and FSII are obtained by an erigeron breviscapus genome cDNA (complementary deoxyribonucleic acid) via primer cloning; genes ADH2, ALD6, ACS and ACCI are obtained by a saccharomyces cerevisiae genome via cloning; recombinant carriers T1 to T5 are constructed by the genes and the primer-amplified promoter and terminator plasmids by a Golden gate cloning technique; bidirectional gene amplification segments of the recombinant carriers are integrated into the conventional integrating sites of the saccharomyces cerevisiae genome by a conventional method, so as to obtain recombinant strains; genes F3'H and CPRI are integrated, recombined with shuttle plasmids, and integrated into chromosomes of the recombinant bacterial strains by homologous arms, so as to obtain the integrated recombinant bacterial strains; the integrated recombinant bacterial strains are screened by an auxotroph culture medium CM-TrP, so as to obtain the luteolin-producing yeast strain. The gene synthesizing method has the characteristics that the yield rate of luteolin is high, and the green and environment-friendly effects are realized.
Owner:CHUXIONG MEDICAL COLLEGE
Rh blood group DEL phenotype RHD838>A allele and detection method thereof
InactiveCN103937806AExtensive scientific research application valueMicrobiological testing/measurementFermentationRhD antigenRegioselectivity
The invention relates to an RHD838>A allele and a detection method thereof, and belongs to the field of analysis and detection technologies in molecular biology. A primer sequence which can amplify a target part including a mutant gene sequence is designed by a PCR-specific primer technology, and PCR amplification selectively and specially aiming at the specific RHD838>A allele is performed on the zone of the target part including the mutant gene sequence by virtue of a gene amplification method. According to the detection method, the gene level is detected by virtue of a molecular biological method which can be used for greatly improving the sensitivity and specificity of the detection and is simple, convenient and rapid. Since different nationalities have difference in the RHD gene, the detection method is designed specially aiming at the RHD838>A allele according to the RHD genetic molecule background of Chinese on the basis of relative researches. The RHD838>A allele and the detection method thereof have significant clinical meaning in overcoming the shortage of the serological technique, and also have wide scientific research and application value.
Owner:WUXI NO 5 PEOPLES HOSPITAL
Method for simultaneously detecting multiple trace sample TCR (T cell receptor) repertoires
ActiveCN105274098AAvoid Frequency Distribution SkewSample requirement is smallMicrobiological testing/measurementDNA/RNA fragmentationComplementary deoxyribonucleic acidGene expansion
The invention discloses a method for simultaneously detecting multiple trace sample TCR (T cell receptor) repertoires. The method includes: taking a sample RNA (ribonucleic acid) as a template to synthesize a first-strand cDNA (complementary deoxyribonucleic acid), performing 5'-terminal RACE PCR, designing specific 5' RACE nested PCR primers and TCR alpha-beta gene specific primers, performing RT-PCR amplification to obtain alpha-beta gene amplification products of each sample TCR, mixing the multiple sample PCR products for sequencing, distinguishing different samples according to joining regions of the specific primers, and analyzing frequency distribution of V and J gene segments in each sample. The method for simultaneously detecting the multiple trace sample TCR repertoires has the advantages that on the premise that distinguishing of different samples is guaranteed, sequencing cost of the TCR repertoires is greatly reduced, and authenticity in frequency distribution of each family is guaranteed; establishment of disease entity RCR repertoires requiring a great quantity of samples is promoted.
Owner:THE FIRST PEOPLES HOSPITAL OF FOSHAN
CRISPR-Cas12a-based streptococcus suis rapid visual RPA detection kit and application thereof
PendingCN113621717AMaintain stabilitySatisfactory reactivityMicrobiological testing/measurementMicroorganism based processesNucleotideTarget gene
The invention discloses a CRISPR-Cas12a-based streptococcus suis rapid visual RPA detection kit and application thereof. The kit comprises a pair of specific primer pairs for amplifying a streptococcus suis cps2j gene, gRNA and a fluorescent report probe, wherein the nucleotide sequences of the primer pairs are shown as SEQ ID NO: 6 and 7 or SEQ ID NO: 8 and 9; and the nucleotide sequence of DNA complementary to the gRNA is shown as SEQ ID NO: 2 or 3. According to the kit, specific Recombinase Polymerase Amplification (RPA) primers and a CRISPR / Cas12a system probe are designed according to the specific gene Cps2j of streptococcus suis, RPA specific target gene amplification and report gene cutting of a CRISPR / Cas12a system are realized, and a rapid visual detection method for the streptococcus suis is established. The method is rapid, visual, good in specificity, high in sensitivity and particularly suitable for detecting the streptococcus suis in resource-deficient areas.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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