111 results about "Molecular diagnostic techniques" patented technology

The invention discloses a nucleotide sequence and a kit for detecting a human epidermal growth factor receptor-2 (HER-2), which belong to the technical field of molecular diagnosis. A primer and a probe for detecting the HER-2 provided by the invention can be used for specifically amplifying and detecting amplification of an HER-2 gene. The invention also discloses the detection kit for detectingthe HER-2 gene based on a microdroplet type PCR (Polymerase Chain Reaction) system, so that the amplification of the HER-2 gene can be quickly, sensitively and accurately detected. The nucleotide sequence and the kit provided by the invention have the advantages of simplicity and convenience in operation, high specificity, high sensibility, low cost, high throughput and the like, can be used for quickly detecting clinic HER-2 gene amplification, and provide references for diagnosis and treatment of the clinic human epidermal growth factor receptor-2.

The invention discloses a multiple touchdown PCR (polymerase chain reaction) detection kit capable of quickly detecting acinetobacter baumannii in lower respiratory tract specimens, belonging to the technical field of molecular diagnosis of infectious diseases of lower respiratory tracts and aiming to solve quick diagnosis of acinetobacter baumannii in lower respiratory tract specimens. The kit comprises 10*PCR buffer solution, MgCl2, dNTP, TaqDNA polymerase, BSA (bovine serum albumin), acinetobacter baumannii positive control DNA and three pairs of acinetobacter baumannii primers. The invention discloses the multiple touchdown PCR detection kit and a detection method capable of detecting acinetobacter baumannii and adopts agarose gel electrophoresis to detect the PCR products. The multiple touchdown PCR detection kit and the detection method have the advantages of quickness, simpleness and convenience, specificity and sensitivity and can be used for quick diagnosis and epidemiological survey of acinetobacter baumannii infection.

The invention relates to a porcine circovirus type 2 rapid typing detection kit which belongs to the field of biological detection and is a kit based on a SMART detection system method. By the adoption of the kit, the technical problem that a porcine circovirus type 2 (PCV2a, 2b, 2d, etc.) typing detection method requires a complex operational program, high experiment skill, long experimental period and the like is solved. A rapid and accurate molecular diagnosis technical product can be provided for porcine circovirus type 2 typing. The kit provided by the invention can be used in system diagnosis of porcine circovirus type 2 rapid typing and used to guide prevention and treatment of the disease caused by porcine circovirus type 2. And mutation detection of each type sequence of the virus can be realized, and the kit is beneficial to molecular epidemiological investigation of the porcine circovirus, meat product quarantine inspection, viral surveillance of forage and water quality in a pig farm and the like.

The invention discloses polygene detection primers for predicting a risk of a distant recurrence of early breast cancer and belongs to the technical field of molecular diagnosis. The polygenes comprise BUB1B, BLM, TPX2, DTX2, PIM1, OBSL1, YWHAB, CLCA2, SF3B5, ESR1 and ERBB2. Primer sequences are shown as SEQ ID NO.1-22. The primers have good specificity, all amplicons generated by a PCR amplification are short, and detection sensitivity of RNA extracted from an FFPE sample can be enhanced. The invention further discloses a method for predicting a risk of a distant recurrence of early breast cancer. The relative expression quantity of the polygenes in the FFPE sample is quantified through SEQ ID NO.1-28, a statistical method of a distant recurrence genetic model is established, the risk of the distant recurrence of the early breast cancer can be predicted, patients with low-risk and high-risk breast cancer are divided, and guidance is provided for treatment of postoperative breast cancer.

The invention belongs to the technical field of molecular diagnosis, and particularly relates to exosome miRNA serving as a molecular marker for diagnosing type 2 diabetes mellitus complicated with coronary heart disease and application of the exosome miRNA. The exosome miRNA is any one or a molecular marker combination of more than two of miR-15a-3p, miR-18a-5p, miR-19a-3p, miR-133a-3p, miR-155-5p and miR-210-3p, and the specific nucleic acid sequence of the exosome miRNA is shown as SEQ NO. 4, SEQ NO. 8, SEQ NO. 11, SEQ NO. 3, SEQ NO. 2 and SEQ NO. 1. Based on a total inventive concept, theinvention also comprises application of the exosome miRNA in preparation of a kit, a microarray or a biochip for diagnosing type 2 diabetes mellitus complicated with coronary heart disease. The invention proves that the six plasma exosome miRNAs can be used as molecular diagnosis markers of the type 2 diabetes mellitus complicated with coronary heart disease, and can be prepared into a kit, a microarray or a biochip for diagnosing the type 2 diabetes mellitus complicated with coronary heart disease, so that the type 2 diabetes mellitus complicated with coronary heart disease can be quickly diagnosed and judged by detecting the expression quantity of the plasma exosome miRNAs of a subject.

The invention provides a product for simultaneously detecting the polymorphism of MTHFR and MTRR genes, as well as a method and application thereof, and relates to the technical field of molecular diagnosis. The primer composition for simultaneously detecting the polymorphism of MTHFR and MTRR genes comprises a primer group for detecting rs 1801133 site, rs1801131 site and rs1801394 site, can be used for simultaneously and comprehensively detecting the polymorphic site of different types of genes with comprehensive detection with strong specificity and high sensitivity, can be used for accurately distinguish three genotypes of each site respectively, and can be used for detecting the lowest concentration of 1ng / mu l. The detection result can be used in clinical directed administration. Furthermore, locked nucleic acid is introduced into a probe for modifying, the probe with enough short length and excellent parting effect can be screened, each parameter of the probe accords with the designing principle, the accuracy of the detection result can be effectively improved, and an optimized parting effect is achieved.

The invention relates to the technical field of molecular diagnosis, and discloses a hypersensitive microRNA electrochemical detection method of an incision enzyme driven multi-legged DNA molecular machine. The method comprises the following steps: aiming at target microRNA, designing and synthesizing detection probes 1, 2, 3 (probe 1, 2, 3) as recognition elements, designing and synthesizing a sulfhydrylation modified probe 4 (probe 4) as a report element, adding the sulfhydrylated probe 4 on the surface of a golden electrode and subjected to incubating overnight in a refrigerator at 4 DEG Cso that a report probe is anchored on the surface of the gold electrode and further a molecular orbit is prepared, identifying through a target sequence and a detection probe, and carrying out cascadeprogrammed reaction to form a trident DNA nano structure, designing a short nucleic acid restriction endonuclease site at each end of the structure as a leg of a molecular machine, and further forming the endonuclease driven multi-legged molecular machine. According to the present invention, the accurate temperature control PCR instrument is not required, the amplification can be achieved under the isothermal condition, and the advantages of simpleness, convenience and hypersensitivity are provided.