39 results about "5-Hydroxymethylcytosine" patented technology

The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and / or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.

The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosine (5hmC) and / or 5-methylcytosine (5meC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC and / or 5meC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA

The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for diagnosing or predicting cancer in a patient. In one embodiment, a method for identifying a patient as having cancer comprises the steps of (a) providing a formalin-fixed, paraffin-embedded or fresh frozen sample of patient tissue; (b) steaming the sample in antigen retrieval buffer; (c) incubating the sample in hydrochloric acid (HCl); (d) incubating the sample with an affinity reagent specific for 5hmC under conditions to form a complex between the affinity reagent and 5-hydroxymethylcytosine (5hmC) present in the sample; (e) detecting the complexes formed between 5hmC and the affinity reagent with secondary detection reagents; (f) quantifying 5hmC levels; and (g) identifying the patient as having cancer if the 5hmC levels in the sample are reduced as compared to a control.

Provided herein are methods for detecting and quantifying 5-hydroxymethylated cytosine bases in a DNA molecule.

The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosme (5hmC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA.

The invention relates to a method and a reagent for adjusting the methylation / demethylation state of nucleic acid. An inventor proves that 5-methylcystein (mC) and 5-hydroxymethylcytosine (hmC) in genome deoxyribonucleic acid (DNA) can be oxidized into 5-carboxyl cytosine (caC) by Tet dioxygenase for the first time. Thymine DNA glycosylase (TDG) can specifically identify the 5caC and remove the 5caC from the genome to ensure that DNA is demethylated actively. Therefore, the methylation / demethylation effect of DNA in the genome can be adjusted, and the reagent for adjusting methylation / demethylation effect of DNA in the genome can be prepared. Through verification, the genome DNA in a cell has 5caC modification, a 5caC modification spectrum can be used for monitoring the state of the cell, and adenosine triphosphate (ATP) and analogues thereof can be used as regulators of Tet enzyme. Moreover, by Tet mutation in leukemia, the 5caC activity is reduced or the 5caC is inactivated.

This invention is related to a method for rapidly quantifying hydroxymethylated DNA by binding DNA to a plastic carrier followed by immunodetection of 5-hydroxymethylcytosine or a hydroxymethylcytosine structure that is marker of DNA hydroxymethylation.

The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosine (5hmC) and / or 5-methylcytosine (5meC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC and / or 5meC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA.

Provided is a sequencing method for the genetic mapping of DNA 5-methylcytosine and 5-hydroxymethylcytosine. The method employs next generation sequencing and library construction technology for trace free DNA and a high-efficiency chemical bioorthogonal reaction, which greatly enhances the selectivity and efficiency of a solid-phase surface binding to a DNA modified base.

Provided are a method and a kit for detecting 5-hydroxymethylcytosine in a nucleic acid. The method is a method for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising the steps of: (1) oxidizing 5-hydroxymethylcytosine in a nucleic acid sample by treating the nucleic acid sample with a tungstic acid-based oxidizing agent comprising peroxotungstic acid, tungstic acid, a salt thereof, or a combination thereof with a reoxidizing agent; and (2) determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample.

The invention discloses a method for identifying and detecting 5-hydroxymethyl cytosine (5hmC) and 5-formyl cytosine (5fC) in DNA and belongs to the fields of molecular biology, nucleic acid chemistryand epigenetics, The method includes: adding to-be-detected DNA into a mixed solution containing potassium perruthenate and alkali, performing reaction under an ice water bath condition to allow the5hmC in the DNA to be oxidized into the 5fC; purifying the DNA after the reaction, adding into a hot alkali solution to perform heat-preservation reaction to achieve DNA cleavage, and performing gel analysis to measure the total number and site of the 5hmC and 5fC in the DNA; directly adding the to-be-detected DNA into the hot alkali solution without potassium perruthenate oxidation to perform heat-preservation reaction to achieve DNA cleavage, and performing gel analysis to measure the total number and site of the 5fC in the DNA; comparing the two results to obtain the number and site of the5hmC and 5fC in the DNA. By the method high in sensitivity and wide in application range, the defects that existing detection methods are high in equipment requirements, complex in operation and the like.