Method for detecting 5-aldehyde cytosine and 5-hydroxymethylcytosine in DNA (deoxyribonucleic acid) by utilizing piperidine aqueous solution
A technology of hydroxymethylcytosine and aldehydecytosine, which is applied in the field of DNA sequencing, can solve the problem of rarely being detected
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Embodiment 1
[0014] Example 1: Reaction of 5-formylcytosine DNA with 5% piperidine
[0015] Take 2 μL of 5-formylcytosine DNA (10 μM), add 93 μL of water and 5 μL of piperidine, heat-treat at 90°C for 0.5 hours, spin the solvent, load the sample to the electrophoresis tank, and observe the gel results after 2 hours , 10% of the 5-formylcytosine DNA was fragmented.
Embodiment 2
[0016] Example 2: Reaction of 5-formylcytosine DNA with 10% piperidine
[0017] Take 2 μL of 5-formylcytosine DNA (10 μM), add 88 μL of water and 10 μL of piperidine, heat-treat at 90°C for 0.5 hours, spin the solvent, load the sample to the electrophoresis tank, and observe the gel results after 2 hours , 35% of the 5-formyl cytosine DNA fragmentation occurred.
Embodiment 3
[0018] Example 3: Reaction of 5-formylcytosine DNA with 15% piperidine
[0019] Take 2 μL of 5-formylcytosine DNA (10 μM), add 83 μL of water and 15 μL of piperidine, heat-treat at 90°C for 0.5 hours, spin the solvent, load the sample to the electrophoresis tank, and observe the gel results after 2 hours , 50% of the 5-formylcytosine DNA fragmentation occurred.
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