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62results about How to "Rapid Detection Analysis" patented technology
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Method, device and equipment for text detection and analysis based on depth neural network
ActiveCN109086756AExact matchImprove accuracyCharacter and pattern recognitionNeural architecturesTemplate matchingText detection
The invention discloses a text detection and analysis method, a device and equipment based on a depth neural network, belonging to the technical field of depth learning and image processing. The method comprises the following steps of: performing template labeling to generate labeling template information; using the preset depth neural network detection model to detect and classify the text regionof the image to be detected, and generating the text region information with categories; template matching being performed according to the label template information and the text area information with categories to generate structured information data. The invention can realize fast and accurate detection and analysis according to various fields in a bill image, has the characteristics of real-time, accuracy, universality, robustness and expandability for the detection and analysis of a document image, and can be widely applied to the field of image text detection, analysis and recognition containing a plurality of texts.
Owner:ZHONGAN INFORMATION TECH SERVICES CO LTD
Multilayer microcavity devices and methods
InactiveUS7144486B1Rapid Detection AnalysisImmobilised enzymesBioreactor/fermenter combinationsConductive materialsPolyimide membrane
Microcavities and micropores that are microscopic (<1 mm) in width and depth and contain any number of individually-addressable electrodes, separated by insulators, along the walls of each cavity. The conducting materials, and the insulator materials can be deposited alternately onto a starting substrate, which is typically an oxidized silicon wafer or polyimide film, but may be any substrate that shows good adhesion to the materials layered on it. The cavities are etched through these layers, perpendicular to the plane of the substrate, exposing the layers at their edges. Pores may be carved entirely through the device.
Owner:SMITHKLINE BECKMAN CORP +1
Multiplex LAMP detection primer, kit and method for six food-borne pathogenic bacteria in fruits and vegetables
InactiveCN107022644AStrong specificityHigh amplification efficiencyMicrobiological testing/measurementMicroorganism based processesEscherichia coliBacteroides
The invention discloses a multiplex LAMP detection primer, kit and method for six food-borne pathogenic bacteria in fruits and vegetables and belongs to the technical field of bacterial gene detection. A rapid detection primer set for the six pathogenic bacteria including listeria monocytogenes, enterobacter sakazakii, shigella spp, staphylococcus aureus, salmonella spp and escherichia coli O157:H7 is designed, and multiplex LAMP reaction is performed on the genome DNA of the bacteria extracted from a sample to be detected in the same reaction system by use of the detection kit including the primer set to determine whether the sample contains the six food-borne pathogenic bacteria or not. The multiplex LAMP detection primer is high in specificity and sensitivity and can accurately detect the genome DNA of the six food-borne pathogenic bacteria in the same reaction system, can realize simple and convenient, quick and accurate detection, is suitable for on-site rapid detection and has significance on improving the pathogenic bacterium analysis and detection technology and the fruit and vegetable edible quality security.
Owner:INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
On-line coal quality laser detection and analysis system
PendingCN106680019AAnalysis and detection are accurateRapid Detection AnalysisWithdrawing sample devicesPreparing sample for investigationNumerical controlEngineering
The invention relates to an on-line coal quality laser detection and analysis system, which comprises an automatic coal-sampling unit, a continuous coal sample-tableting unit and a coal laser detection and analysis unit that are connected in sequence through cables, a conveyer belt and a manipulator; the conveyer belt connects the automatic coal-sampling unit with a pulverized coal sample bin of the continuous coal sample-tableting unit; the continuous coal sample-tableting unit comprises a sample-tableting disc, and a hydraulic head and a filling port are arranged on the top of the sample-tableting disc; a pulverized coal sample feed station and a filling station are arranged on the sample-tableting disc, and a pulverized coal tablet ejection station is arranged under the sample-tableting disc; the coal laser detection and analysis unit comprises a three-dimensional point location numerical control platform, a travelling unit is connected to the three-dimensional point location numerical control platform, and an optical focusing element, a pulsed laser and a laser detection and analysis instrument are arranged on the top of the three-dimensional point location numerical control platform. The on-line coal quality laser detection and analysis system can implement automatic sampling, automatic sample tableting and automatic laser detection and analysis in the process of coal transportation.
Owner:山东利普斯智慧能源科技有限公司
Kit for high-sensitivity detection on proteoglycan and preparation method of kit
ActiveCN105606827AHigh sensitivityRapid Detection AnalysisBiological testingMonoclonal antibodyPolyclonal antibodies
The invention relates to a kit for high-sensitivity detection on proteoglycan and a preparation method of the kit. The kit comprises carboxyl graphene coupled with a monoclonal antibody and / or carboxyl graphene coupled with a polyclonal antibody, fluorescent proteins with high-positive charges, and a buffering solution. The invention further relates to a preparation method of the kit. According to the kit for high-sensitivity detection on the proteoglycan, an elisa plate is not needed to be adsorbed and wrapped by an antibody, and such complicated steps of washing antigen and antibody combination and the like are eliminated; and furthermore, quick detection and analysis on target proteoglycan in a homogeneous system can be realized, and the sensitivity is high.
Owner:SHANDONG UNIV
Core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material, and preparation method and application thereof
InactiveCN103433007AEasy to operateWide applicabilityComponent separationOther chemical processesRhodamine 6GOrganic solvent
The invention discloses a core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material, and a preparation method and application thereof. According to the molecularly imprinted solid phase extraction magnetic material, surface-aminated Fe3O4 nano-particles serve as a magnetic core, and a rhodamine 6G molecularly imprinted polymer membrane serves as a shell. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has a memory function for a three-dimensional structure of rhodamine 6G molecules, and rhodamine 6G can be specifically and selectively separated and enriched. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material is applied to selective adsorption and dynamic extraction and separation of a trace amount of rhodamine 6G in the food sample analysis pretreatment; the rhodamine 6G can be quickly separated and detected; according to the method, the recovery rate is high and the relative standard deviation is small; the core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has the characteristics of small using amount of organic solvents, simplicity, quickness and high efficiency in operation, and the like.
Owner:GUANGXI UNIV FOR NATITIES
Wide-range electron beam excited fluorescence imaging and spectral measurement device and method thereof
ActiveCN108267430AHigh resolution imagingImprove fluorescence collection efficiencyFluorescence/phosphorescenceMeasurement deviceElectron
The invention discloses a wide-range electron beam excited fluorescence imaging and spectral measurement device and a method thereof. The wide-range electron beam excited fluorescence imaging and spectral measurement device comprises a electron microscope scanning system, a scanning signal generator, a wide-range fluorescence collecting, coupling and transmitting system, a fluorescence intensity detector, a fluorescence spectral detector, a synchronous scanning signal collector and a cooperative control and data processing output system. Since a wide-range fluorescence coupler is introduced, fluorescence signals excited within an imaging view field by the microscope scanning system can be converged and coupled to a fluorescence transmission light path with high collection efficiency, the problems that, to images acquired by wide-range fluorescence scanning imaging, it is difficult to calculate and compare fluorescence excitation intensity or fluorescence yield and fluorescence spectruminformation of different positions according to uniform standards are solved, and wide-range rapid detection and analysis on the basis of electron beam excited fluorescence signals can be completed.
Owner:PEKING UNIV
Detection device and method of non-methane total hydrocarbon
InactiveCN109765318ARapid Detection AnalysisSimple processComponent separationDual columnEngineering
The invention belongs to the technical field of the gas detection, and relates to a detection device and method of non-methane total hydrocarbon. The detection device comprises a multi-channel sampling valve, a first quantitative loop, a second quantitative loop and a detector; switchable quantitative mode and detection mode are formed through the multi-channel sampling valve; the quantitative mode is a sample gas channel including the first quantitative ring and the second quantitative ring; the detection mode is two carrier gas channels; one carrier gas channel comprises the first quantitative ring and a methane column, and the other carrier gas channel comprises the second quantitative ring and a total hydrocarbon column; and two carrier gas channels are connected with a detector. Through the detection device and method of non-methane total hydrocarbon provided by the invention, the detection of the non-methane total hydrocarbon can be realized since a fourteen-way valve is adoptedto conform to the dual column in the HJ38-2017 standard, the flow is simplified, the problem that the existing non-methane total hydrocarbon detection device is complex in structure can be solved at the same time, and the detection device has the advantages of being simple in structure, and convenient for installation and operation.
Owner:NANJING HOPES TECH
Microplastic detection device and method based on pyrolysis-mass spectrometry technology
ActiveCN109738507AReduce distractionsReduce volumePreparing sample for investigationMaterial analysis by electric/magnetic meansPyrolysis mass spectrometryComputer science
The invention relates to a microplastic detection device and method based on a pyrolysis-mass spectrometry technology, aiming to solve the technical problem that a microplastic content research devicein the prior art is complex in structure and inconvenient to operate, cannot accurately perform quantitative analysis on components in microplastics, is easily disturbed by the environment, has largeerrors and low accuracy, wastes time and labor, has a large instrument occupancy area, can only be operated in labs, and is difficult to be applied to on-site detection. According to the microplasticdetection device based on the pyrolysis-mass spectrometry technology provided by the invention, the whole device is a closed gas path device system connected by pipelines, and the devices are sequentially connected, including a working gas source, a pyrolysis device, a filter and a mass spectrometer; after a microplastic sample is put into the pyrolysis device, the pyrolysis device performs pyrolysis on the microplastic sample, and pyrolysis products of the microplastic sample are driven by working carrier gas and filtered by the filter to enter the mass spectrometer for detection and analysis; and the invention also provides a microplastic detection method thereof. The scheme of the invention is widely applied to the technical field of microplastic detection.
Owner:HARBIN INST OF TECH AT WEIHAI
Electron beam excitation fluorescence large-range direct detection imaging apparatus and a method thereof
ActiveCN108279247AImprove fluorescence collection efficiencyImprove the efficiency of experimental testingMaterial analysis using wave/particle radiationPhotometry using electric radiation detectorsPhotodetectorFluorescence
The invention discloses an electron beam excitation fluorescence large-range direct detection imaging apparatus and a method thereof. The imaging apparatus comprises a scanning electron microscope system, a scanning signal generator, a fluorescence collection coupling system, a semiconductor photodetector, a scanning synchronization signal collector and a synergic control and data processing output system. According to the present invention, by using the modular architecture, the configuration adjustment and the subsequent upgrade of each module are flexible and convenient; and by introducingthe large-area semiconductor photoelectric detection chip of the semiconductor photodetector, the fluorescence excited by the scanning electron microscope system in the large imaging visual field range can be concentrated and coupled to the semiconductor photodetector at the same high collection efficiency, such that the problem that the fluorescence excitation intensities or the fluorescence excitation yields at different positions of the image obtained through the large-range fluorescence scanning imaging are difficultly calculated and compared by using the standard is solved so as to complete the large-range rapid detection and analysis based on the electron beam excitation fluorescence signals.
Owner:北京金竟科技有限责任公司
Preparation and detection methods of fluorescence sensor for aflatoxin toxin production gene nor-1
The invention discloses a preparation method and detection method of a fluorescence sensor for an aflatoxin toxin production gene nor-1. The fluorescence sensor is a long-arm hairpin probe based on specific recognition of the aflatoxin toxin production gene nor-1; and the two ends of the long-arm hairpin probe are provided with NH2 and SH correspondingly, a CdTe / CdS quantum dot of an upper core-shell structure is modified by the NH2, and upper gold nanoparticles (AuNPs) are modified by the SH. The preparation method comprises the steps: a CdTe / CdS quantum dot solution is prepared for standby application; the AuNPs are prepared for standby application; and the long-arm hairpin probe is modified. Detection comprises construction of the fluorescence sensor, when the target nor-1 appears, thenor-1 and the hairpin probe are subjected to specific complementary pairing, the hairpin probe is straightened, the distance between the CdTe / CdS quantum dot and the AuNPs is enlarged, transferring offluorescent resonance energy is blocked, thus fluorescence of the CdTe / CdS quantum dot is recovered, the concentration of the nor-1 is tested through the fluorescence recovery intensity, and thus thenor-1 is quickly detected. The prepared fluorescence sensor has the advantages that flexibility, simpleness, quickness and the like are achieved, the toxin production gene is detected from the source, the cost is low, and detection is quick.
Owner:JIANGSU UNIV OF SCI & TECH
Method for constructing fluorescence resonance energy transfer sensor and method for detecting CaMV35S promoter by means of sensor
InactiveCN106053415AClear design ideasThe synthesis method is simpleFluorescence/phosphorescenceCvd grapheneFörster resonance energy transfer
The invention provides a method for constructing a fluorescence resonance energy transfer (FRET) sensor based on graphene and quantum dots and a method for detecting a CaMV35S promoter by means of the sensor. The method comprises the steps of 1, preparing multiwalled carbon nanotubes / graphene oxide nanoribbons (MWCNTs / GONRs) dispersion liquid; 2, preparing nitrogen sulfur heterotactic graphene quantum dots (NSGQDs) solution; 3, preparing nitrogen sulfur heterotactic graphene quantum dots-capture probe (NSGQDs-ssDNA) dispersion liquid; 4, constructing the FRET sensor; 5, constructing a standard curve; 6, detecting transgenic soybean by means of the FRET sensor constructed based on MWCNTs / GONRs-NSGQDs-ssDNA. The FRET sensor has the advantages of being convenient and flexible to operate and high in analysis speed and is suitable for quick detection and analysis.
Owner:JIANGSU UNIV
Concealed channel detection method based on multi-scale flow analysis technology
ActiveCN111586075AReduce false alarm rateReduce false negative rateCharacter and pattern recognitionData switching networksData packInternet traffic
The invention discloses a concealed channel detection method based on a multi-scale flow analysis technology, and the method comprises the following steps: obtaining the information of a detection andanalysis scene, and collecting the network traffic data in the detection and analysis scene through DPDK; preprocessing the collected network traffic data to obtain a single data packet, a session flow and a communication flow; establishing a corresponding meta-database according to the related characteristics of the single data packet, the session flow and the communication flow; importing the obtained single data packet, session flow and communication flow into the corresponding meta-database for black and white matching to obtain priori knowledge; establishing a multi-scale feature analysis model; and importing the obtained single data packet, session flow and communication flow into the multi-scale feature analysis model for analysis and detection, and generating a detection report. According to the invention, the false alarm rate and missing report rate of concealed channel detection can be effectively reduced, the comprehensiveness and accuracy of concealed channel detection areimproved, and the transmission security of network information is ensured.
Owner:NAT COMP NETWORK & INFORMATION SECURITY MANAGEMENT CENT +1
Low-molecular-weight substance mass spectrometry detection method based on palladium-gold core-shell micro-nano material
ActiveCN111458399AReduce manufacturing costThe synthesis steps are simpleMaterial analysis by electric/magnetic meansSerum samplesPhysical chemistry
The invention discloses a low-molecular-weight substance mass spectrometry detection method based on a palladium-gold core-shell micro-nano material, and particularly relates to application of the low-molecular-weight substance mass spectrometry detection method in serum low-molecular-weight substance detection. The palladium-gold alloy core-shell micro-nano particles are used as a matrix material, a serum sample does not need to be subjected to any pretreatment steps such as enrichment or separation, and low-molecular-weight substances in serum can be efficiently and quickly detected and analyzed by taking a very small amount of sample. The detection method is high in accuracy, low in cost and high in detection flux, meets the requirements of clinical serum detection, and has clinical application potential.
Owner:SHANGHAI JIAO TONG UNIV
Method for rapidly detecting and analyzing quality of sericite
InactiveCN105699298ARapid Detection AnalysisComprehensive detection and analysisColor/spectral properties measurementsMaterial analysis using radiation diffractionSpectrum analyzerX-ray
The invention discloses a method for rapidly detecting and analyzing the quality of sericite. The method comprises the following steps: acquiring a physical structure of a sericite sample to be detected by adopting an X-ray diffraction analyzer; acquiring the element content of the sericite sample to be detected by adopting a microcomputer multi-element analyzer, and acquiring spectral information of the sericite sample to be detected by adopting a spectrum analyzer; analyzing the physical structure, element content and spectral information of the sericite sample to be detected by adopting an optimal correction model, so as to obtain a quality analysis result of the sericite sample to be detected, wherein a process of establishing the optimal correction model comprises the following step: S1: acquiring the physical structures of the plurality of sericite samples by the X-ray diffraction analyzer under the conditions that the working voltage is 38kV-42kV, the working current is 28mA-32mA and the wavelength is 10-20 angstroms, so as to obtain information of the plurality of physical structures. The method disclosed by the invention can be used for comprehensively and rapidly detecting and analyzing the quality of the sericite, and is low in cost and high in detection and analysis efficiency.
Owner:ANHUI HENGHAO SCI & TECH
Primer and probe for real-time fluorescent PCR detection of transgenic alfalfa J163 strains and detection method and applications thereof
ActiveCN104673886AStrong specificityEasy to operateMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyFluorescent pcr
The invention discloses a primer and a probe for the real-time fluorescent PCR detection of transgenic alfalfa J163 strains and a detection method and applications thereof. For transgenic alfalfa J163 strains which are provided by Monsanto Company in America and do not have the Security Certificate of Agricultural Genetically Modified Organisms awarded by the Ministry of Agriculture in China, the invention provides a pair of specific primer and probe for the first time, through the optimization of real-time fluorescent PCR detection systems, a specific real-time fluorescent PCR detection system for alfalfa J163 strains is successfully established, and the system is simple in operation, good in specificity and repeatability, accurate in detection results and high in stability, detects that the lowest DNA concentrations is 15 pg, and can quickly, accurately and steadily carry out detection analysis and authentication on the components of transgenic alfalfa J163 strains.
Owner:黄埔出入境检验检疫局综合技术服务中心
High performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for determination of indaziflam and metabolites of indaziflam
The invention discloses a high performance liquid chromatography tandem mass spectrometry (HPLC-MS / MS) method for the determination of indaziflam and metabolites of the indaziflam. The method comprises the steps as follows: establishing a standard working curve of a soil matrix of a known concentration gradient of indaziflam and four metabolites of the indaziflam by the HPLC-MS / MS; determining theresidual amount of the indaziflam and the four metabolites of the indaziflam in a soil sample extraction and purification liquid by the HPLC-MS / MS, recording peak areas of extracted ion chromatograms, quantifying the matrix with an external standard method, obtaining measured values of the indaziflam and the four metabolites of the indaziflam in the soil sample extraction and purification liquid; and then substituting the measured values into a quantitative calculation formula to finally obtain the residual amount of the indaziflam to be detected and the four metabolites of the indaziflam insoil samples. The method provided by the invention has the advantages that the sample pretreatment operation is simple and convenient, the dosage of an organic solvent is small, the target pesticideextraction rate is high, the purification effect is good, and the instrument analysis time is short. In addition, the instrument detection limit and the method detection limit are very low.
Owner:PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
Parallel light micro-spot optical key dimension analysis device and detection method
InactiveCN107345788AOvercoming time-consuming problemsRapid Detection AnalysisUsing optical meansElectronic structureGrating
The present invention discloses a parallel light micro-spot optical key dimension detection method. With the detection method adopted, a vertical sidewall structure and a non-vertical sidewall structure in a microelectronic structure can be analyzed. According to the detection method, a parameter-of-interest curve of a sample is detected through a spectrograph; a microelectronic periodic structure physical model of key dimension information such as the grating period, duty ratio and grating thickness of the structure of the sample is built to perform simulation calculation, so that the zero-order diffraction rate curve of the sample can be obtained; fitting analysis is performed on the zero-order diffraction rate curve which is obtained through simulation and a zero-order diffraction rate curve detected by the spectrograph; and the key dimensions of the microelectronic periodic structure to be tested are obtained through inversion. The present invention discloses a detection device for the above method. The detection device can detect and analyze the vertical sidewall structure and non-vertical sidewall structure in the microelectronic structure and can detect and analyze the non-vertical sidewall structure quickly. The method and device can quickly detect and analyze the non-vertical sidewall structure.
Owner:FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
An electron beam excited fluorescence large-scale direct detection imaging device and method thereof
ActiveCN108279247BImprove fluorescence collection efficiencyImprove the efficiency of experimental testingMaterial analysis using wave/particle radiationPhotometry using electric radiation detectorsFluorescencePhotodetector
Owner:北京金竟科技有限责任公司
ONOO<-> spectroscopic probe, preparation method thereof and method for measuring ONOO<-> by ultraviolet visible absorption spectrum and fluorescence spectrum
ActiveCN109970636AHigh yieldHigh sensitivityOrganic chemistryColor/spectral properties measurementsFluorescenceFiltration
The invention discloses a spectroscopic probe for detecting peroxynitrite ion (ONOO<->), a preparation method of the spectroscopic probe, and a method for measuring the ONOO<-> by an ultraviolet visible absorption spectrum and a fluorescence spectrum, and belongs to the technical field of ONOO<-> detection. The synthetic method of the ONOO<-> spectroscopic probe comprises the following steps: performing heating reflux on 1,2-diaminoanthraquinone and quinoline-2-formaldehyde in a methanol solution, performing suction filtration, performing washing, and performing drying to obtain the spectroscopic probe. The ONOO<-> probe provided by the invention can quickly detect the ONOO<-> in a high-selectivity high-sensitivity manner, and the method is simple and convenient to operate.
Owner:SHANXI UNIV
Boiler water-cooled wall temperature distribution rapid detection, analysis and evaluation system
InactiveCN111896112AQuick judgmentRapid Detection AnalysisThermometer detailsTemperature measurement of flowing materialsHeat mapData information
The invention discloses a boiler water-cooled wall temperature distribution rapid detection, analysis and evaluation system. The system comprises the following steps: cleaning the surface of a water-cooled wall pipe; positioning and marking: numbering the wall pipes in the horizontal counterclockwise direction and marking the numbered wall pipes; collecting and recording wall temperature data of the water-cooled wall: moving a thermal infrared imager to shoot the water-cooled wall, wherein an obtained heat map comprises two marks; water wall pipe position identification: automatically identifying and judging the arrangement and position distribution of the wall pipes through marks on the heat map; analyzing the wall temperature: automatically extracting the highest temperature, the lowesttemperature and the average temperature of each water wall pipe by a computer; alarming when the wall temperature of the water-cooled wall is abnormal: the system gives an alarm when the wall temperature is abnormal; report generation and processing: selecting data and information needing to be formed into a report from a database, and automatically generating the report; and alarm query statistics: querying alarm record data according to different categories. The method is simple and accurate in wall pipe number positioning and identification, can quickly identify, extract and sort out the heat map, and is low in cost and high in accuracy.
Owner:JIANGSU FRONTIER ELECTRIC TECH
Sample pretreatment and amplification integrated nucleic acid analysis system based on pipette tip and application of system
ActiveCN104593252ALow priceEasy to operateBioreactor/fermenter combinationsHeating or cooling apparatusBiologySolid phase extraction
The invention relates to a sample pretreatment and amplification integrated nucleic acid analysis system based on a pipette tip and the application of the system. According to the system, a nucleic acid extraction card integrated on the pipette tip is mainly used for pretreating the sample, and the nucleic acid is extracted by use of a solid-phase extraction method; next, a hand-held heating table is utilized to perform nucleic acid amplification on a nucleic acid reaction liquid; in addition, an ultraviolet lamp is used for visually judging and reading the result of detection by use of naked eyes; as a result, the real-time detection and the field detection of the nucleic acid sample are realized. The nucleic acid analysis system can be widely applied to nucleic acid detection and analysis in the fields of environmental detection, disease diagnosis, inspection andquarantine and medicolegal expertise.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
High-flux drug detection micro-fluidic chip and application
PendingCN107213927AStrong adsorption capacityGood detection effectLaboratory glasswaresBiological testingAntigenMicrofluidic chip
The invention provides a high-flux drug detection micro-fluidic chip. The high-flux drug detection micro-fluidic chip consists of a substrate layer, a modification layer and a reaction layer, wherein the reaction layer consists of n reaction modules; each reaction module consists of an entrance, a reaction channel and an outlet. The chip modified by polylysine is hydrophilic, facilitates a sample to be detected to flow into the chip, and has an adsorption effect on drug complete antigens; the polylysine of the modification layer is matched with PDMS (Polydimethylsiloxane) of the reaction layer, so that the hydrophilic feature of the polylysine can be combined with the high-protein adsorption feature of the PDMS to achieve a better detection effect; detection of different samples occurs in different reaction channels, so that the phenomenon of mutual interference is avoided, and non-specific adsorption is lowered; a single chip is provided with a plurality of reaction modules to realize high-flux rapid result detection and analysis; the sizes, intervals and number of the reaction modules can be adjusted as required to realize simultaneous field detection of a plurality of samples and drugs, so that different detection requirements are met.
Owner:ZHEJIANG UNIV +1
Antibody composition for detecting PD-1 receptor on lymphocyte surface and application thereof
PendingCN114133449ARapid Detection AnalysisImprove throughputBiological material analysisImmunoglobulins against cell receptors/antigens/surface-determinantsAntiendomysial antibodiesLymphocyte
The invention provides an antibody composition for detecting a PD-1 receptor on the surface of lymphocyte and application of the antibody composition. The antibody composition for detecting the PD-1 receptor on the surface of lymphocyte comprises an anti-PD-1 antibody and any one or a combination of at least two of an anti-CD45 antibody, an anti-CD3 antibody, an anti-CD4 antibody and an anti-CD8 antibody. The invention also provides a kit for detecting the PD-1 receptor on the surface of the lymphocyte and a method for detecting the expression rate of the PD-1 receptor on the surface of the lymphocyte. The antibody composition for detecting the PD-1 receptor on the surface of the lymphocyte can be used for rapidly and accurately detecting PD-1 expression conditions of various types of lymphocytes in cooperation with flow cytometry, operation is easy, detection efficiency is high, continuous detection and dynamic observation of related samples are achieved, and a basis is provided for related drug treatment.
Owner:THE FIRST AFFILIATED HOSPITAL OF XIAMEN UNIV +1
Detection method of residual sodium thiocyanate in milk
InactiveCN106290337ARealize preliminary judgmentHigh precisionMaterial analysis by observing effect on chemical indicatorMilk sampleSodium thiocyanate
The invention discloses a detection analysis method of sodium thiocyanate. The method comprises the following steps: (1) preparing a sodium thiocyanate standard solution; (2) dividing milk samples into a detection sample and a sample to be labeled, adding the sodium thiocyanate standard solution into the sample to be labeled to obtain a labeled sample; (3) respectively adding a sodium thiocyanate detection reagent A into the detection sample and the labeled sample, shaking for uniformly mixing, collecting supernatant, adding a sodium thiocyanate reagent B, and uniformly shaking; and (4) comparing the detection sample with the labeled sample, observing and analyzing the color of the detection sample relative to the labeled sample, and determining the content of the sodium thiocyanate in the detection sample by virtue of a labeling amount of the labeled sample. The monitoring analysis method integrates the high speed of an ordinary kit detection method and high precision of a chromatographic analysis method, and has good effect for improving the detection and analysis efficiency and detection and analysis precision.
Owner:四川新华西乳业有限公司
Accurate and rapid organophosphorus pesticide detection method for vegetables
InactiveCN110007024AStable in natureReduce the amount addedComponent separationOrganophosphorus pesticidesSilicon dioxide
The invention discloses an accurate and rapid organophosphorus pesticide detection method for vegetables. The method comprises the following steps: preparing a standard sample solution; evenly mixingacetone and cyclohexane to obtain eluent; putting vegetables and silicon dioxide into a grinding machine to be ground and smashed with the grinding pressure being 0.12-1. 0.18 MPa and with the grinding time being 20-20 min. 40 minutes to obtain a sample; sequentially adding anhydrous sodium sulfate, a sample and anhydrous sodium sulfate to the bottom of the injector, compacting, leaching with theeluent, flowing out through a needle hole part of the injector, collecting the eluent, volatilizing the eluent in a ventilation cabinet until the eluent is dry, adding acetone for dissolving, and fixing the volume to obtain a to-be-detected solution; and analyzing by using a gas chromatography detector. The method disclosed by the invention is simple, high in detection sensitivity, high in reproducibility and high in detection speed.
Owner:ANHUI SANLIAN UNIV
Automatic pollination device for fruit trees
InactiveCN110810235AImprove accuracyGuaranteed accuracyAgriculturePlant genotype modificationFruit treePollination
The invention relates to an automatic pollination device for fruit trees, which comprises a base seat, an unmanned aerial vehicle and pollination equipment, wherein the pollination equipment carries out pollination operation under the traction of the unmanned aerial vehicle; the base seat comprises movable wheels, a tracker and the like; the tracker is used for tracking the coordinates of the unmanned aerial vehicle in a horizontal position and the like, controlling the movable wheels to roll according to the coordinates of the unmanned aerial vehicle in the horizontal position to control thebase seat to move to the ground position directly below the unmanned aerial vehicle or closest to the unmanned aerial vehicle, the wireless power supply coil is arranged at the top end of the telescopic rod, the first control unit controls the telescopic rod to perform telescopic operation, controls the movable wheels to move; and the pollen bin is used for storing a plurality of pollen boxes. Pollen and electric energy can be supplemented without interruption in the pollination process, so that the pollination time is saved; and meanwhile, multi-directional pollination detection is carried out, so that the pollination accuracy is improved, the pollination is quickly supplemented, and the pollination efficiency is improved.
Owner:LIANYUNGANG ACAD OF AGRI SCI
Preparation method and micromolecule detection application of metal organic framework material
PendingCN113484404AAvoid defectsHigh sensitivityMaterial analysis by electric/magnetic meansMetal chlorideAlcohol
The invention discloses a preparation method and micromolecule detection application of a metal organic framework material, and relates to the field of metal organic framework materials. The preparation method comprises the steps that: dimethylformamide, ethyl alcohol, deionized water, triethylamine and terephthalic acid are mixed and added into a closed container, and metal chloride is added, so that a mixture can be formed; centrifuging and washing are performed after water bath ultrasonic treatment; then dispersing is performed in deionized water, and low-speed centrifugal separation is performed to obtain the ultrathin metal organic framework material; and the ultrathin metal organic framework material is applied to small molecule detection. According to the framework material and the method, the metal ions and the thickness in the metal organic framework material can be accurately adjusted, the defects of a traditional matrix are overcome, the sample size is low, and serum small molecules can be rapidly detected with high throughput and high sensitivity.
Owner:SHANGHAI JIAO TONG UNIV
onoo-spectroscopy probe and preparation method, and method for measuring onoo- by ultraviolet-visible absorption spectrum and fluorescence spectrum
ActiveCN109970636BHigh yieldHigh sensitivityOrganic chemistryColor/spectral properties measurementsUv vis absorbanceQuinoline
Owner:SHANXI UNIV
A mass spectrometric detection method for low molecular weight substances based on palladium-gold core-shell micro-nano materials
ActiveCN111458399BReduce manufacturing costThe synthesis steps are simpleMaterial analysis by electric/magnetic meansSerum samplesPhysical chemistry
Owner:SHANGHAI JIAOTONG UNIV
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