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A mass spectrometric detection method for low molecular weight substances based on palladium-gold core-shell micro-nano materials

A micro-nano material, low molecular weight technology, applied in the field of mass spectrometry detection of low molecular weight substances, can solve the problems of cumbersome pretreatment steps, long time consuming, and difficulty in achieving low cost of serum, and achieve low cost, low preparation cost and high accuracy. Effect

Active Publication Date: 2021-10-01
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the cumbersome and time-consuming pretreatment steps of gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, it is difficult to achieve low-cost, large-sample analysis and detection of serum and apply it to clinical practice
Compared with the above two mass spectrometry methods, matrix-assisted laser desorption time-of-flight mass spectrometer has the characteristics of simple sample preparation and high analysis efficiency, but it has extremely high requirements for the matrix

Method used

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  • A mass spectrometric detection method for low molecular weight substances based on palladium-gold core-shell micro-nano materials
  • A mass spectrometric detection method for low molecular weight substances based on palladium-gold core-shell micro-nano materials
  • A mass spectrometric detection method for low molecular weight substances based on palladium-gold core-shell micro-nano materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: the detection of glucose standard substance

[0055] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometer, using reflection mode, positive ion detection; prepared micro-nano particles; prepared glucose standard solution;

[0056] (2) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0057] (3) Detect under the mass spectrometer, and analyze the mass spectrum image, and the test results are as follows: Figure 4 shown.

Embodiment 2

[0058] Embodiment 2: the detection of lysine standard substance

[0059] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometer, using reflection mode, positive ion detection; prepared micro-nano particles; prepared lysine standard solution;

[0060] (2) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0061] (3) Detect under the mass spectrometer, and analyze the mass spectrum image, and the test results are as follows: Figure 5 shown.

Embodiment 3

[0062] Example 3: Detection of Small Molecules in Serum Samples

[0063] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometer, using reflection mode, positive ion detection; prepared palladium-gold alloy core-shell micro-nano particles;

[0064] (2) Take 0.1 microliter of serum and dilute it 10 times to prepare a serum sample;

[0065] (3) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0066] (4) Detect under the mass spectrometer, and analyze the mass spectrum image, and the test results are as follows: figure 1 shown.

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Abstract

The invention discloses a mass spectrometry detection method for low molecular weight substances based on palladium-gold core-shell micro-nano materials, and specifically relates to its application in the detection of serum low molecular weight substances. In the present invention, palladium-gold alloy core-shell micro-nano particles are used as the matrix material, and serum samples do not need to go through any pretreatment steps such as enrichment or separation, and each sample is sampled very little, so that the low molecular weight in serum can be detected and analyzed efficiently and quickly. substance. This detection method has high accuracy, low cost, and high detection throughput, which meets the needs of clinical serum detection and has the potential for clinical application.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry detection of low molecular weight substances, in particular to a detection method and application of serum low molecular weight substances or metabolite mass spectrometry based on palladium-gold core-shell micro-nano materials. Background technique [0002] Diagnosis is the key to screening cancer and can guide clinical treatment. Notably, blood test-based diagnosis offers desirable sensitivity and specificity, is low cost, and is noninvasive compared to imaging and biopsy methods. Metabolic profiling is an emerging precision medicine tool that can detect pathway end products. However, due to sample complexity and low metabolite abundance in patient specimens, metabolic profiling is still insufficient for diagnostic applications. Therefore, there is an urgent need to construct new metabolic analysis tools for the clinical application of cancer screening and treatment. [0003] Compare...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
CPCG01N27/628
Inventor 钱昆曹敬黄琳苏海洋张梦吉
Owner SHANGHAI JIAOTONG UNIV
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