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onoo-spectroscopy probe and preparation method, and method for measuring onoo- by ultraviolet-visible absorption spectrum and fluorescence spectrum

A fluorescent probe and probe technology are applied in the fields of ONOO- spectral probes and preparation, fluorescence spectroscopic determination of ONOO-, and ultraviolet-visible absorption spectroscopy, and can solve the problems of unfavorable rapid detection and analysis, cumbersome operation process, poor identification, and the like, Achieve the effect of cheap and easy-to-obtain reagents, quick and easy operation, and high accuracy

Active Publication Date: 2022-05-20
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Colorimetric detection of ONOO is now possible - The fluorescent probes are very few, and in the prior art, the detection of ONOO - However, there are problems of low sensitivity, poor accuracy, cumbersome operation process, and poor recognition, which is not conducive to rapid detection and analysis.

Method used

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  • onoo-spectroscopy probe and preparation method, and method for measuring onoo- by ultraviolet-visible absorption spectrum and fluorescence spectrum
  • onoo-spectroscopy probe and preparation method, and method for measuring onoo- by ultraviolet-visible absorption spectrum and fluorescence spectrum
  • onoo-spectroscopy probe and preparation method, and method for measuring onoo- by ultraviolet-visible absorption spectrum and fluorescence spectrum

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Experimental program
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Effect test

Embodiment 1

[0028] Synthesis and characterization of embodiment 1 fluorescent probe L

[0029] Fluorescent probe L was obtained by heating 1,2-diaminoanthraquinone and quinoline-2-carbaldehyde in a methanol solution at 65 °C for 24 h under reflux, then cooling, suction filtration, washing and drying.

[0030] 1H NMR (600 MHz, DMSO) δ 8.52 (s, 1H), 8.23 ​​(d, J = 22.1 Hz, 1H), 8.20 (s, 1H), 8.11 (s, 1H), 7.99 (s, 1H), 7.88 (s, 1H), 7.80 (s, 1H), 7.74(s, 1H), 7.66 – 7.62 (m, 1H), 7.62 – 7.55 (m, 1H), 7.47 (s, 1H), 6.78 (s,1H ), 6.38 (s, 1H), 3.33 (s, 2H). HRMS (ESI): calcd. for C 24 h 15 N 3 o 2 (L+H) 378.12, found 378.12344.

[0031] The reaction formula is:

[0032] .

Embodiment 2

[0033] Embodiment 2 fluorescent probe L is used for ONOO - UV absorbance determination

[0034] Take 2 mL of DMSO:phosphate buffer solution (4:1) containing 1 mM fluorescent probe and add it to a clean colorimetric tube, and detect it on a UV-Vis spectrophotometer, with ONOO - The addition of , the color of the solution changed from pink to golden yellow, and a new absorption peak appeared at 477nm; with ONOO - With the increase, the maximum absorbance detected on the UV-Vis spectrophotometer gradually shifted to the left and enhanced to see figure 1 .

Embodiment 3

[0035] Embodiment 3 fluorescent probe L is used for ONOO - Fluorescence spectrometry

[0036] Take 2 mL of DMSO:Phosphate buffer solution (4:1) containing 1 mM fluorescent probe and add it to a clean colorimetric tube, and detect it on a fluorescence photometer, with ONOO - The addition of , the emission peak at 632 nm disappeared, and a new absorption peak gradually appeared at 590 nm; with ONOO - The increase of the maximum absorption peak detected on the fluorescence photometer gradually shifted to the left and enhanced see figure 2 .

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Abstract

The invention discloses a method for detecting peroxynitrite ion (ONOO ‑ ) Spectral probe and preparation method and UV-Vis absorption spectrum, fluorescence spectrum determination of ONOO ‑ The method belongs to ONOO ‑ detection technology field, ONOO ‑ The synthesis method of the spectral probe is obtained by heating 1,2-diaminoanthraquinone and quinoline-2-formaldehyde in a methanol solution to reflux, and then suction filtering, washing and drying. ONOO of the present invention ‑ Probe can rapidly detect ONOO with high selectivity and high sensitivity ‑ , Simple and convenient operation.

Description

technical field [0001] The present invention relates to ONOO - The technical field of preparation and detection, specifically relates to a kind of ONOO - Spectral probe and preparation method, and UV-visible absorption spectrum, fluorescence spectrum determination of ONOO - Methods. Background technique [0002] ONOO - It is a strong oxidant that acts on macromolecules such as enzymes, proteins, lipids, and DNA to produce cytotoxic effects, causing cell metabolism disorders and energy depletion, leading to cell apoptosis, damage, and even death. [0003] Colorimetric detection of ONOO is now possible - The fluorescent probes are very few, and in the prior art, the detection of ONOO - However, there are problems of low sensitivity, poor accuracy, cumbersome operation process, and poor identification, which is not conducive to rapid detection and analysis. Contents of the invention [0004] The object of the present invention is to provide a kind of preparation and det...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D215/12C09K11/06G01N21/33G01N21/64
CPCC07D215/12C09K11/06G01N21/33G01N21/6428G01N21/643C09K2211/1011C09K2211/1029
Inventor 李明璐宋胜梅梁帆李甘董川双少敏
Owner SHANXI UNIV
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