46 results about "Peroxynitrous acid" patented technology

The present invention provides a method for oxidizing a substance (e.g., in a waste stream, drinking water, a paper pulp slurry, or on a surface), which uses free radicals and reactive species generated from multiple oxidants. The method comprises combining peroxynitrite or peroxynitrous acid and at least one additional oxidizing agent for a period of time sufficient to oxidize the substance of interest. The peroxynitrite or peroxynitrous acid preferably is formed by irradiation of nitrate ion and / or nitric acid (e.g., with UV or gamma rays). The yield of free radicals and reactive species, which are the intermediate species that perform the oxidation may be increased by addition of a catalysts, electromagnetic radiation, sonic waves, and / or electrolysis.

The present invention provides a method for treating wounds and diseases in mammals, caused by mammalian cells involved in an inflammatory response, by altering indigenous in vivo levels of peroxynitrous acid, and salts thereof. The method comprises contacting the mammalian cells with a therapeutically effective amount of a reactive oxygen species mediator, wherein the reactive oxygen species mediator is selected from the group consisting of pyruvates, pyruvate precursors, α-keto acids having four or more carbon atoms, precursors of α-keto acids having four or more carbon atoms, and the salts thereof, wherein mediation of reactive oxygen species results in mediation of peroxynitrous acid. The present invention further provides a pharmaceutical composition for treating wounds and diseases in mammals, caused by mammalian cells involved in an inflammatory response, by altering indigenous in vivo levels of peroxynitrous acid, and salts thereof.

The invention relates to a novel rare-earth complex ratiometric fluorescent probe which can be used for measuring peroxynitrite in a biosystem. The probe is complexes formed by two trivalent rare-earth ions Eu<3+> and Tb<3+> and an organic ligand [4'-(2,4-dimethoxyphenyl)-2,2':6'2''-terpyridine-6,6''-dimethylamino]tetraacethyl (DTTA for short), wherein the structural formula of the ligand is disclosed in the specification. The probe can specifically act with the peroxynitrite, so that the two rare-earth complexes are changed at the ratio (F612 / F541) of the fluorescence intensity of 612nm and 541nm, thereby implementing the selective fluorescent measurement of the peroxynitrite in a biosystem.

The invention provides a fluorescent probe for detecting peroxynitrite in a cell. The structural formula of the fluorescent probe is shown in the description, wherein R1 and R2 are selected from hydrogen, linear chain alkyl containing 1 to 5 carbon atoms or naphthenic base containing 3 to 8 carbon atoms respectively and separately; R3 is selected from a cyano group or an ester group. The fluorescent probe has high peroxynitrite selectivity, high cell permeability, extremely-short response time, extremely high detection sensitivity and no toxic and side effects to the cell per se, and is suitable for detecting the concentration change of the peroxynitrite in the cell. The fluorescent probe synthetic method is simple, is high in yield, and is suitable for large-scale production.

The invention discloses a two-photon fluorescence probe for detecting peroxynitrite as well as a preparation method and application of the two-photon fluorescence probe. The two-photon fluorescence probe has the structural formula as shown in the description. The two-photon fluorescence probe is named as TPNIR-FP. The two-photon fluorescence probe adopts a Nile red derivative as a fluorescent group and an alpha-keto-amide functional group as an ONOO-reaction group, can rapidly respond to ONOO- (within 10 seconds), and in addition is high in sensitivity and good in selectivity. The two-photon fluorescence probe can be applied to imaging research on ONOO- in antharcycline antibiotic induced cardiac muscle cell and cardiac muscle tissue damage.

The invention discloses a peroxynitrite ion detection probe as well as a preparation method and application thereof. The peroxynitrite ion detection probe comprises a fluorescent nano material and an enzyme, wherein the enzyme is combined with the fluorescent nano material, has an oxidation-reduction property and can be selectively oxidized by peroxynitrite ions to form an oxidized enzyme; after the fluorescent nano material is radiated by excited light, electrons in an excited state can be transferred to the oxidized enzyme and cannot turn back to a base state, and then fluorescent light of the fluorescent nano material can be quenched. As an inorganic light-emitting material is adopted as fluorophore, the peroxynitrite ion detection probe disclosed by the invention is intense in light emission, good in stability and less prone to photo-bleach; the surface of the probe is modified with the enzyme with oxidation-reduction property, and the enzyme can be selectively oxidized by ONOO<->, so that the probe is not interfered by other active oxygen (ROS) or active nitrogen (RNS), and is excellent in specificity.

The invention provides a peroxynitrite ion detection probe. The molecular formula of the peroxynitrite ion detection probe AH-1 is C20H15NO2. The invention further discloses a preparation method and application of the peroxynitrite ion detection probe. The molecular structure of the peroxynitrite ion detection probe provided by the invention comprises a 9,10-dihydracridine (DHAC) response unit; the probe does not have fluorescent light; when the probe reacts with peroxynitrite ions, a fluorescent product can be produced; furthermore, the detection process has the characteristics of quick response, sensitivity and high selectivity; meanwhile, the probe is easy to synthesize and modify and extremely high in biocompatibility and can be used for detecting intrinsic type peroxynitrite ions in a biological system.

The invention belongs to the technical field of synthesis and detection, and particularly relates to a fluorescent probe for detecting peroxynitrite and a preparation method and application thereof. The invention provides a universal identification group for detecting peroxy nitrite, and three new fluorescent probes which are respectively named as BT-FAP, NA-FAP and NR-FAP are formed by connectingthe universal identification group with common fluorophores, wherein aminonaphthalene (BT), naphthalimide (NA) and benzophenoxazine (NR) are the fluorophores, and formamide (FAP) is the identification group to achieve the instant reaction with ONOO (all 4s), the fluorescent probe has high sensitivity and good selectivity, and is very suitable for ONOO fluorescence imaging studies in cells.

An object of the present invention is to provide an antioxidant composition that is effective in the living body against active oxygen species produced in the body. The present invention provides an antioxidant composition having the effect of suppressing three active oxygen species, i.e. hypochlorite radicals, hydroxyl radicals, and peroxynitrite radicals, with respect to active oxygen species produced in the body, the antioxidant composition comprising a mixture in which at least 20 mg of vitamin C (L-ascorbic acid or sodium L-ascorbate) as an agent to scavenge peroxynitrite active oxygen, and at least 2 mg of caffeic acid analogue compound(s), at least 10 mg of polyphenyol compound(s) or at least 1.5 mg of carotenoid compound(s) as an agent to scavenge hydroxyl radical active oxygen are combined with 100 mg of histidine-containing dipeptide, or sulfur-containing amino acids or analogues thereof obtained from animal extract as an agent for scavenging hypochlorite active oxygen.

The invention belongs to the technical field of fluorescence spectrum detection and analysis, and particularly relates to a visual organic molecular fluorescence probe on the basis of cyanine and a method for preparing the visual organic molecular fluorescence probe. The visual organic molecular fluorescence probe comprises a cyanine fluorophore and benzothiazole groups. The method includes stepsof (1), dropwise adding phosphoryl chloride into N, N'-dimethylformamide to obtain mixtures, then adding the mixtures into bromoacetic acid and carrying out reaction to obtain products II; (2), carrying out reaction on 2, 3, 3'-trimethyl indole and ethyl iodide to obtain products III; (3), dropwise adding piperidine into the products II and the products III and carrying out reaction to obtain products IV; (4), carrying out reaction on the products IV and aminothiophenol at the room temperature to obtain the visual organic molecular fluorescence probe. The visual organic molecular fluorescenceprobe and the method have the advantages that obvious color change of the visual organic molecular fluorescence probe can be seen in natural light or ultraviolet lamps, linear relations between the fluorescence intensity and peroxynitrite negative ions are established, and accordingly the peroxynitrite negative ions can be qualitatively and quantitatively detected; the visual organic molecular fluorescence probe is good in selectivity and high in sensitivity, detection methods are easy to implement, and obvious effects can be realized.

The invention discloses a phosphorescent polymer probe capable of detecting peroxynitrite by using a ratio method and a preparation method and application thereof. Two types of phosphorescent iridiumcomplexes (Ir1 and Ir2) and a water-soluble polymer are copolymerized to form the phosphorescent polymer probe, wherein the phosphorescent iridium complex (Ir1) is an inert phosphorescent reference unit, which does not respond to peroxynitrite; as a phosphorescent response unit, the phosphorescent iridium complex (Ir2) can chemically react with peroxynitrite, thereby realizing specific response toperoxynitrite; and as a main chain formation unit for the polymer, the water-soluble polymer endows the polymer with good water solubility and biocompatibility. The phosphorescent polymer probe P-ONOO can be used in the detection and imaging of peroxynitrite in aqueous solutions, living cells and living bodies by the ratio method, and has an important application prospect in terms of biological imaging and sensing.

The invention discloses an orange fluorescent carbon dot and application thereof in detection of peroxynitrite ions. The carbon dot (CDs) is prepared from rhodamine B and p-aminophenol as raw materials through a one-step hydrothermal synthesis method; the carbon dot is a zero-dimensional carbon nanomaterial and can emit bright orange fluorescence. The preparation method is simple and convenient, and the prepared carbon dots are good in water solubility and stable in fluorescence property. The peroxynitrite ions can be rapidly and sensitively detected by taking the carbon dots as a fluorescentprobe. When peroxynitrite ions exist, fluorescence of the carbon dots is effectively quenched by peroxynitrite. Meanwhile, the color of the CDs solution is changed into blue black from pink. Comparedwith a pure fluorescence analysis method, the analysis method for detecting the ONOO<-> has the advantages that a dual-mode output method has more advantages in practical application, and it is indicated that the analysis method has potential application prospects in discussion of the effect of the ONOO <-> in cytobiology.

The invention discloses a nano composite system with nitric oxide / photodynamic synergistic antibacterial and anti-inflammatory effects as well as a preparation method and application of the nano composite system. The nano composite system comprises up-conversion nanoparticles of a core-shell structure, mesoporous silica wrapping the up-conversion nanoparticles, an AIE photosensitizer, an NO donor and modified polyethylene glycol which are loaded on the mesoporous silica. According to the nano composite system, the purpose of the synergy of two treatment modes triggered by one laser is achieved, the generated active oxygen, nitric oxide and peroxynitrite anions can be efficiently utilized to kill bacteria while the high biological safety is maintained, and the excellent antibacterial and anti-inflammatory effects are provided in the treatment of the S.aures infected bacterial keratitis model, and therefore the nano composite system has the potential of becoming an alternative strategy for treating bacterial keratitis.

The invention discloses a mitochondria-targeted peroxynitrite / bisulfite double-response fluorescent probe, and belongs to the technical field of chemical biological materials. The invention aims to solve the problems that most probes lack biological targeting and cannot detect two molecules at the same time, so that the relationship between two or more micromolecules and a specific disease in a living body cannot be deeply studied and the pathogenesis of the micromolecules cannot be studied. The peroxynitrite / bisulfite double-response fluorescent probe, disclosed by the invention, can target mitochondria; the preparation method of the fluorescent probe comprises the following steps: dissolving m-diethylaminophenol and sodium hydride in N, N-dimethylformamide under the protection of nitrogen, and stirring at room temperature; then adding a near-infrared dye IR-780 into the reaction liquid, and heating to react; and concentrating the reaction liquid, and carrying out column chromatography separation to obtain a target compound NI, namely the peroxynitrite / bisulfite double-response fluorescent probe.

The invention provides a preparation method and application of near-infrared (NIR) luminous biomass quantum dots (BQDs), and a NIR ratio fluorescent probe that specifically responds to peroxynitrite (ONOO<->). The preparation method of the NIR ratio fluorescent probe comprises: first preparing a chlorophyll crude extract from Holly leaves, then preparing the biomass quantum dots (HL-BQDs), and finally preparing the NIR ratio fluorescent probe: HL-BQDs-Cy7. The NIR ratio fluorescent probe provided by the invention has a specific response to ONOO<->, shows high sensitivity and good selectivity on detection of the ONOO<-> content in cell mitochondria, and realizes ratio fluorescence imaging of endogenous ONOO<-> of mice in vivo.

The present invention provides a method for treating wounds and diseases in mammals, caused by mammalian cells involved in an inflammatory response, by altering indigenous in vivo levels of peroxynitrous acid, and salts thereof. The method comprises contacting the mammalian cells with a therapeutically effective amount of a reactive oxygen species mediator, wherein the reactive oxygen species mediator is selected from the group consisting of pyruvates, pyruvate precursors, α-keto acids having four or more carbon atoms, precursors of α-keto acids having four or more carbon atoms, and the salts thereof, wherein mediation of reactive oxygen species results in mediation of peroxynitrous acid. The present invention further provides a pharmaceutical composition for treating wounds and diseases in mammals, caused by mammalian cells involved in an inflammatory response, by altering indigenous in vivo levels of peroxynitrous acid, and salts thereof.

The invention discloses a ratio-type nano photoacoustic detection probe and a preparation method and an application thereof. The ratio-type nano photoacoustic detection probe comprises poloxamer, anda naphthalocyanine dye and a heptamethine cyanine fluorescent small molecule supported on the poloxamer. The probe of the invention has high sensitivity and can specifically react with the peroxynitrite anion to change the ultraviolet-to-infrared absorption intensity ratio (A863 / A780) of the probe at 863 and 780 nm and change the photoacoustic signal ratio (PA860 / PA775) at 860 and 775nm, and thereby achieves selective detection and imaging of peroxynitrite anion in tumor parts of animals. In addition, the preparation process of the invention is simple, no complex and expensive apparatus is required to prepare the probe, and the preparation is simple and easy.

The invention discloses a prussian blue-based nanoprobe, and a preparation method and an application of the prussian blue-based nanoprobe in detection and imaging of peroxynitrite ionsand belongs to the technical field of biosensors. The nano-probe is any one of a PB nano-probe and a CuS-coated PB nano-probe, and the structural formula of the nano-probe is shown in the specification, through the two nanoprobes, PAI and MRI dual-mode imaging is realized by changing a valence state of iron ions and the structure of PB under the oxidation effect of ONOO <->, detection and imaging with high sensitivity, good specificity and no penetration depth limitation on ONOO <-> are realized, and problems of poor penetration depth and low specificity of a conventional probe are greatly improved. The method is advantaged in that the PB nanoprobe and the CuS-coated PB nanoprobe are prepared through a simple method, the preparation process is short, operation is simple, and cost is low.

An object of the present invention is to provide an antioxidant composition that is effective in the living body against active oxygen species produced in the body. The present invention provides an antioxidant composition having the effect of suppressing three active oxygen species, i.e. hypochlorite radicals, hydroxyl radicals, and peroxynitrite radicals, with respect to active oxygen species produced in the body, the antioxidant composition comprising a mixture in which at least 20 mg of vitamin C (L-ascorbic acid or sodium L-ascorbate) as an agent to scavenge peroxynitrite active oxygen, and at least 2 mg of caffeic acid analogue compound(s), at least 10 mg of polyphenyol compound(s) or at least 1.5 mg of carotenoid compound(s) as an agent to scavenge hydroxyl radical active oxygen are combined with 100 mg of histidine-containing dipeptide, or sulfur-containing amino acids or analogues thereof obtained from animal extract as an agent for scavenging hypochlorite active oxygen.

The invention provides an application of rosmarinic acid as a peroxynitrite inhibitor. The invention further provides the application of rosmarinic acid in the preparation of drugs for curing and preventing atherosclerotic lesions, acute or chronic inflammation, ischemia reperfusion injury and neurodegenerative diseases caused by peroxynitrite. The invention selects rosmarinius officinalis, which is Chinese herbal medicine having medicinal value, as the research object, and the effective active component of rosmarinius officinalis is separated and purified to obtain the rosmarinic acid. The rosmarinic acid can effectively inhibit the bio-macromolecule damage induced by peroxynitrite, particularly, the rosmarinic acid can effectively inhibit the DNA chain breakage induced by peroxynitrite with the inhibition ratio reaching 40% under low concentration and the rosmarinic acid can further inhibit the protein tyrosine nitration and the low-density lipoprotein lipid peroxidation induced by peroxynitrite with the inhibition ratio reaching higher than 50% under low concentration.

The invention discloses a spectroscopic probe for detecting peroxynitrite ion (ONOO<->), a preparation method of the spectroscopic probe, and a method for measuring the ONOO<-> by an ultraviolet visible absorption spectrum and a fluorescence spectrum, and belongs to the technical field of ONOO<-> detection. The synthetic method of the ONOO<-> spectroscopic probe comprises the following steps: performing heating reflux on 1,2-diaminoanthraquinone and quinoline-2-formaldehyde in a methanol solution, performing suction filtration, performing washing, and performing drying to obtain the spectroscopic probe. The ONOO<-> probe provided by the invention can quickly detect the ONOO<-> in a high-selectivity high-sensitivity manner, and the method is simple and convenient to operate.

The invention relates to a chemical reagent and a method for detecting biological endogenous oxidants, in particular to a compound shown as a general formula I or II and a method for detecting the biological endogenous oxidants such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) by using the compound. The compound has high selectivity particularly for peroxynitrous acid (ONOO-) and hypochlorous acid (ClO-) and can be applied to a cell microscopic imaging experiment, a fluorescent dye can be generated, and a sensitive, proportional and multi-channel fluorescent signal can be sent.

The invention relates to a novel rare-earth complex ratiometric fluorescent probe which can be used for measuring peroxynitrite in a biosystem. The probe is complexes formed by two trivalent rare-earth ions Eu<3+> and Tb<3+> and an organic ligand [4'-(2,4-dimethoxyphenyl)-2,2':6'2''-terpyridine-6,6''-dimethylamino]tetraacethyl (DTTA for short), wherein the structural formula of the ligand is disclosed in the specification. The probe can specifically act with the peroxynitrite, so that the two rare-earth complexes are changed at the ratio (F612 / F541) of the fluorescence intensity of 612nm and 541nm, thereby implementing the selective fluorescent measurement of the peroxynitrite in a biosystem.

The invention relates to a chemical reagent and a method for detecting biological endogenous oxidants, in particular to a compound shown as a general formula I or II and a method for detecting the biological endogenous oxidants such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) by using the compound. The compound has high selectivity particularly for peroxynitrous acid (ONOO-) and hypochlorous acid (ClO-) and can be applied to a cell microscopic imaging experiment, a fluorescent dye can be generated, and a sensitive, proportional and multi-channel fluorescent signal can be sent.

The invention belongs to the technical field of fluorescence spectrum detection and analysis, and particularly relates to a visual organic molecular fluorescence probe on the basis of cyanine and a method for preparing the visual organic molecular fluorescence probe. The visual organic molecular fluorescence probe comprises a cyanine fluorophore and benzothiazole groups. The method includes stepsof (1), dropwise adding phosphoryl chloride into N, N'-dimethylformamide to obtain mixtures, then adding the mixtures into bromoacetic acid and carrying out reaction to obtain products II; (2), carrying out reaction on 2, 3, 3'-trimethyl indole and ethyl iodide to obtain products III; (3), dropwise adding piperidine into the products II and the products III and carrying out reaction to obtain products IV; (4), carrying out reaction on the products IV and aminothiophenol at the room temperature to obtain the visual organic molecular fluorescence probe. The visual organic molecular fluorescenceprobe and the method have the advantages that obvious color change of the visual organic molecular fluorescence probe can be seen in natural light or ultraviolet lamps, linear relations between the fluorescence intensity and peroxynitrite negative ions are established, and accordingly the peroxynitrite negative ions can be qualitatively and quantitatively detected; the visual organic molecular fluorescence probe is good in selectivity and high in sensitivity, detection methods are easy to implement, and obvious effects can be realized.

The invention provides a fluorescent probe for detecting peroxynitrite ions. The fluorescent probe provided by the invention has high specificity, is not interfered by other components in a corresponding peroxynitrite ion detection process, can be used for real-time determination of peroxynitrite ions in living cells, amd has wide application prospect. The fluorescent probe for detecting the peroxynitrite ions in cells is high in sensitivity and good in fluorescence emission spectrum characteristic, wherein the peroxynitrite ions in cells are determined by drawing a standard curve, so that thepurpose of rapid and accurate detection of the peroxynitrite ions in the cells can be achieved.

The invention discloses a ratio-type nano-photoacoustic detection probe and its preparation method and application. The ratio-type nano-photoacoustic detection probe includes a poloxamer and naphthalocyanine loaded on the poloxamer Dyes and Heptamethine-like Fluorescent Small Molecules. The probe of the present invention has high sensitivity, and can specifically interact with peroxynitrite anion, so that the ultraviolet-near-infrared absorption intensity ratio (A863 / A780) of the probe at 863 and 780nm is changed, and at 860 and 775nm The photoacoustic signal ratio (PA860 / PA775) at the site changes, thereby realizing the selective detection and imaging of the peroxynitrite anion in the tumor site in the animal. In addition, the preparation process of the present invention is simple, does not require complicated and expensive instruments to prepare the probe, and is simple and easy.