46 results about "Peroxynitrous acid" patented technology

The invention discloses a nano composite system with nitric oxide / photodynamic synergistic antibacterial and anti-inflammatory effects as well as a preparation method and application of the nano composite system. The nano composite system comprises up-conversion nanoparticles of a core-shell structure, mesoporous silica wrapping the up-conversion nanoparticles, an AIE photosensitizer, an NO donor and modified polyethylene glycol which are loaded on the mesoporous silica. According to the nano composite system, the purpose of the synergy of two treatment modes triggered by one laser is achieved, the generated active oxygen, nitric oxide and peroxynitrite anions can be efficiently utilized to kill bacteria while the high biological safety is maintained, and the excellent antibacterial and anti-inflammatory effects are provided in the treatment of the S.aures infected bacterial keratitis model, and therefore the nano composite system has the potential of becoming an alternative strategy for treating bacterial keratitis.

The present invention provides a method for treating wounds and diseases in mammals, caused by mammalian cells involved in an inflammatory response, by altering indigenous in vivo levels of peroxynitrous acid, and salts thereof. The method comprises contacting the mammalian cells with a therapeutically effective amount of a reactive oxygen species mediator, wherein the reactive oxygen species mediator is selected from the group consisting of pyruvates, pyruvate precursors, α-keto acids having four or more carbon atoms, precursors of α-keto acids having four or more carbon atoms, and the salts thereof, wherein mediation of reactive oxygen species results in mediation of peroxynitrous acid. The present invention further provides a pharmaceutical composition for treating wounds and diseases in mammals, caused by mammalian cells involved in an inflammatory response, by altering indigenous in vivo levels of peroxynitrous acid, and salts thereof.

An object of the present invention is to provide an antioxidant composition that is effective in the living body against active oxygen species produced in the body. The present invention provides an antioxidant composition having the effect of suppressing three active oxygen species, i.e. hypochlorite radicals, hydroxyl radicals, and peroxynitrite radicals, with respect to active oxygen species produced in the body, the antioxidant composition comprising a mixture in which at least 20 mg of vitamin C (L-ascorbic acid or sodium L-ascorbate) as an agent to scavenge peroxynitrite active oxygen, and at least 2 mg of caffeic acid analogue compound(s), at least 10 mg of polyphenyol compound(s) or at least 1.5 mg of carotenoid compound(s) as an agent to scavenge hydroxyl radical active oxygen are combined with 100 mg of histidine-containing dipeptide, or sulfur-containing amino acids or analogues thereof obtained from animal extract as an agent for scavenging hypochlorite active oxygen.

The invention discloses a method for detecting peroxynitrite ion (ONOO ‑ ) Spectral probe and preparation method and UV-Vis absorption spectrum, fluorescence spectrum determination of ONOO ‑ The method belongs to ONOO ‑ detection technology field, ONOO ‑ The synthesis method of the spectral probe is obtained by heating 1,2-diaminoanthraquinone and quinoline-2-formaldehyde in a methanol solution to reflux, and then suction filtering, washing and drying. ONOO of the present invention ‑ Probe can rapidly detect ONOO with high selectivity and high sensitivity ‑ , Simple and convenient operation.

The invention relates to a novel rare-earth complex ratiometric fluorescent probe which can be used for measuring peroxynitrite in a biosystem. The probe is complexes formed by two trivalent rare-earth ions Eu<3+> and Tb<3+> and an organic ligand [4'-(2,4-dimethoxyphenyl)-2,2':6'2''-terpyridine-6,6''-dimethylamino]tetraacethyl (DTTA for short), wherein the structural formula of the ligand is disclosed in the specification. The probe can specifically act with the peroxynitrite, so that the two rare-earth complexes are changed at the ratio (F612 / F541) of the fluorescence intensity of 612nm and 541nm, thereby implementing the selective fluorescent measurement of the peroxynitrite in a biosystem.

The invention relates to a chemical reagent and a method for detecting biological endogenous oxidants, in particular to a compound shown as a general formula I or II and a method for detecting the biological endogenous oxidants such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) by using the compound. The compound has high selectivity particularly for peroxynitrous acid (ONOO-) and hypochlorous acid (ClO-) and can be applied to a cell microscopic imaging experiment, a fluorescent dye can be generated, and a sensitive, proportional and multi-channel fluorescent signal can be sent.

The invention belongs to the technical field of fluorescence spectrum detection and analysis, and particularly relates to a visual organic molecular fluorescence probe on the basis of cyanine and a method for preparing the visual organic molecular fluorescence probe. The visual organic molecular fluorescence probe comprises a cyanine fluorophore and benzothiazole groups. The method includes stepsof (1), dropwise adding phosphoryl chloride into N, N'-dimethylformamide to obtain mixtures, then adding the mixtures into bromoacetic acid and carrying out reaction to obtain products II; (2), carrying out reaction on 2, 3, 3'-trimethyl indole and ethyl iodide to obtain products III; (3), dropwise adding piperidine into the products II and the products III and carrying out reaction to obtain products IV; (4), carrying out reaction on the products IV and aminothiophenol at the room temperature to obtain the visual organic molecular fluorescence probe. The visual organic molecular fluorescenceprobe and the method have the advantages that obvious color change of the visual organic molecular fluorescence probe can be seen in natural light or ultraviolet lamps, linear relations between the fluorescence intensity and peroxynitrite negative ions are established, and accordingly the peroxynitrite negative ions can be qualitatively and quantitatively detected; the visual organic molecular fluorescence probe is good in selectivity and high in sensitivity, detection methods are easy to implement, and obvious effects can be realized.

The invention discloses a peroxynitrite ion detection probe as well as a preparation method and application thereof. The peroxynitrite ion detection probe comprises a fluorescent nano material and an enzyme, wherein the enzyme is combined with the fluorescent nano material, has an oxidation-reduction property and can be selectively oxidized by peroxynitrite ions to form an oxidized enzyme; after the fluorescent nano material is radiated by excited light, electrons in an excited state can be transferred to the oxidized enzyme and cannot turn back to a base state, and then fluorescent light of the fluorescent nano material can be quenched. As an inorganic light-emitting material is adopted as fluorophore, the peroxynitrite ion detection probe disclosed by the invention is intense in light emission, good in stability and less prone to photo-bleach; the surface of the probe is modified with the enzyme with oxidation-reduction property, and the enzyme can be selectively oxidized by ONOO<->, so that the probe is not interfered by other active oxygen (ROS) or active nitrogen (RNS), and is excellent in specificity.

The invention provides a fluorescent probe for detecting peroxynitrite in a cell. The structural formula of the fluorescent probe is shown in the description, wherein R1 and R2 are selected from hydrogen, linear chain alkyl containing 1 to 5 carbon atoms or naphthenic base containing 3 to 8 carbon atoms respectively and separately; R3 is selected from a cyano group or an ester group. The fluorescent probe has high peroxynitrite selectivity, high cell permeability, extremely-short response time, extremely high detection sensitivity and no toxic and side effects to the cell per se, and is suitable for detecting the concentration change of the peroxynitrite in the cell. The fluorescent probe synthetic method is simple, is high in yield, and is suitable for large-scale production.

The invention discloses a ratio-type nano-photoacoustic detection probe and its preparation method and application. The ratio-type nano-photoacoustic detection probe includes a poloxamer and naphthalocyanine loaded on the poloxamer Dyes and Heptamethine-like Fluorescent Small Molecules. The probe of the present invention has high sensitivity, and can specifically interact with peroxynitrite anion, so that the ultraviolet-near-infrared absorption intensity ratio (A863 / A780) of the probe at 863 and 780nm is changed, and at 860 and 775nm The photoacoustic signal ratio (PA860 / PA775) at the site changes, thereby realizing the selective detection and imaging of the peroxynitrite anion in the tumor site in the animal. In addition, the preparation process of the present invention is simple, does not require complicated and expensive instruments to prepare the probe, and is simple and easy.