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Near-infrared luminous biomass quantum dots and NIR ratio fluorescent probe, and preparation method and application of biomass quantum dots and fluorescent probe

A ratiometric fluorescent probe, proton technology, used in the fields of chemistry and biomedicine, can solve the problems of unsuitable imaging detection, poor selectivity, and expensive experimental equipment

Active Publication Date: 2020-01-17
GUANGXI NORMAL UNIV
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Problems solved by technology

Currently detected ONOO - There are many methods, including electron paramagnetic resonance spectroscopy (EPR), ultraviolet-visible absorption spectroscopy, chromatography, etc. The existing detection methods are either expensive in experimental equipment, or have low sensitivity due to the detection of substances. Poor stability, and not suitable for ONOO in cells and in vivo - Imaging detection and other issues

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  • Near-infrared luminous biomass quantum dots and NIR ratio fluorescent probe, and preparation method and application of biomass quantum dots and fluorescent probe
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  • Near-infrared luminous biomass quantum dots and NIR ratio fluorescent probe, and preparation method and application of biomass quantum dots and fluorescent probe

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[0046] In order to understand the above objects, features and advantages of the present invention more clearly, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that the embodiments of the present application and the features in the embodiments may be combined with each other in the case of no conflict.

[0047] Many specific details are set forth in the following description to facilitate a full understanding of the present invention. However, the present invention can also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited by the specific implementation disclosed below. example limitations.

[0048] A specific embodiment provided by the first aspect of the present invention provides a preparation method of near-infrared (NIR) luminescent biological mass dots, which is characterized by ...

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Abstract

The invention provides a preparation method and application of near-infrared (NIR) luminous biomass quantum dots (BQDs), and a NIR ratio fluorescent probe that specifically responds to peroxynitrite (ONOO<->). The preparation method of the NIR ratio fluorescent probe comprises: first preparing a chlorophyll crude extract from Holly leaves, then preparing the biomass quantum dots (HL-BQDs), and finally preparing the NIR ratio fluorescent probe: HL-BQDs-Cy7. The NIR ratio fluorescent probe provided by the invention has a specific response to ONOO<->, shows high sensitivity and good selectivity on detection of the ONOO<-> content in cell mitochondria, and realizes ratio fluorescence imaging of endogenous ONOO<-> of mice in vivo.

Description

technical field [0001] The invention relates to the technical fields of chemistry and biomedicine, in particular to materials for biological information detection in organisms and cells, in particular to near-infrared luminescent biological mass quantum dots and NIR ratio fluorescent probes, and preparation methods and applications thereof. Background technique [0002] peroxynitrite (ONOO - ) is an important reactive oxygen species in the body, which is composed of nitric oxide free radicals (NO ) and superoxide anion free radicals (O 2 - ·) Rapid reaction generation, the site of which is mainly in the mitochondria in the cell. Thanks to ONOO - It has strong oxidative and nucleophilic properties, and can react with various proteins, liposomes, nucleic acids, etc., thereby causing cell damage. [0003] Accurate determination of ONOO - content, which can provide precise biological information for the interpretation of ONOO at the molecular level - The relevant injury me...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/06C01B32/15B82Y20/00B82Y30/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/06C09K11/65C09K2211/1007C09K2211/1029C01B32/15G01N21/6428
Inventor 赵书林林鹏翔张亮亮赵晶瑾黄勇
Owner GUANGXI NORMAL UNIV
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