Nucleic acid analysis cassette and nucleic acid analysis equipment

Abstract

The invention discloses a nucleic acid analysis cassette and nucleic acid analysis equipment. The nucleic acid analysis cassette comprises a cassette body and a magnetic bead transferring component which is movably arranged on the cassette body along a set direction, wherein the cassette body is provided with at least two liquid storage chambers, sample application passages, exhaust passages and adetection structure, the at least two liquid storage chambers are distributed sequentially along a set direction; and the magnetic bead transferring component is provided with magnetic bead enrichingstructures, the liquid storage chambers, the sample application passages, the exhaust passages and the detection structure are all located at the same side of the magnetic bead transferring component, a surface where the liquid storage chambers are located is in tight connection with the magnetic bead transferring component, and the magnetic bead transferring component can enable the magnetic bead enriching structures to communicate with each liquid storage chamber sequentially. According to the nucleic acid analysis cassette, magnetic-bead-method-based nucleic acid extraction and amplified detection can be fully integrated, the compactness of the nucleic acid analysis cassette is improved, the size of the nucleic acid analysis cassette is reduced, and the popularization of molecular diagnosis technologies is promoted.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, more specifically, to a nucleic acid analysis cartridge and nucleic acid analysis equipment. Background technique [0002] In the field of in vitro diagnosis, molecular diagnosis is a commonly used technology. Specifically, molecular diagnosis is a diagnostic technology that uses nucleic acid or protein as a biomarker for clinical detection, providing a basis for judgment, diagnosis, prevention and treatment of diseases. Especially in the severe situation of various sudden infectious diseases, molecular diagnostic technology can provide fast and accurate diagnosis, which is the most economical and effective. [0003] At present, the nucleic acid extraction technology based on the magnetic bead method is a common technology for nucleic acid extraction. The operation process requires various liquids such as lysate, magnetic bead binding liquid, cleaning liquid, and eluent, and is supplem...

AI Technical Summary

Problems solved by technology

Automated nucleic acid extraction equipment often requires complex liquid manipulation components, magnetic bead manipulation components, and detection devices, resulting in a large volume of equipment, which is difficult to meet the needs of areas with backward medical infrastructure and on-site instant detection

[0004] In addition, the combination of automated nucleic acid extraction and amplification detection has the risk of interface cross-contamination

Images