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Exosome miRNA as molecular marker for diagnosing type 2 diabetes mellitus complicated with coronary heart disease and application thereof

A technology for molecular markers and type 2 diabetes, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of inspection risks and high costs

Active Publication Date: 2021-03-30
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Abstract
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Problems solved by technology

[0003]At present, the gold standard for clinical diagnosis of coronary heart disease is coronary angiography, but because of its high cost, invasiveness, and inspection risks, there are defects, so the research and development A non-invasive, rapid, sensitive and low-cost molecular diagnostic method is imperative

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  • Exosome miRNA as molecular marker for diagnosing type 2 diabetes mellitus complicated with coronary heart disease and application thereof
  • Exosome miRNA as molecular marker for diagnosing type 2 diabetes mellitus complicated with coronary heart disease and application thereof
  • Exosome miRNA as molecular marker for diagnosing type 2 diabetes mellitus complicated with coronary heart disease and application thereof

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Embodiment

[0036] Plasma from 6 healthy controls and 6 patients with type 2 diabetes and coronary heart disease were collected and stored at -80°C in time. The basic clinical characteristics of the collectors are shown in Table 1.

[0037] Table 1 The basic clinical characteristics of the collectors

[0038]

[0039] Note: Continuous variables are mean ± standard deviation (mean ± SE), and categorical variables are expressed as percentages. CTR, healthy controls; DM-IHD, diabetic patients with coronary heart disease.

[0040] Exosomes were extracted from the above plasma samples using the Exoquick kit (System Biosciences), and the obtained exosomes were fixed on the sample-loading copper grid. After staining and drying, the morphology of the obtained exosomes was analyzed using a JEOL-1230 transmission electron microscope. Identify; and use ZetaView PMX 110 particle tracker and Western blot to analyze and identify the particle size and specific markers of the obtained exosomes. Among...

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Abstract

The invention belongs to the technical field of molecular diagnosis, and particularly relates to exosome miRNA serving as a molecular marker for diagnosing type 2 diabetes mellitus complicated with coronary heart disease and application of the exosome miRNA. The exosome miRNA is any one or a molecular marker combination of more than two of miR-15a-3p, miR-18a-5p, miR-19a-3p, miR-133a-3p, miR-155-5p and miR-210-3p, and the specific nucleic acid sequence of the exosome miRNA is shown as SEQ NO. 4, SEQ NO. 8, SEQ NO. 11, SEQ NO. 3, SEQ NO. 2 and SEQ NO. 1. Based on a total inventive concept, theinvention also comprises application of the exosome miRNA in preparation of a kit, a microarray or a biochip for diagnosing type 2 diabetes mellitus complicated with coronary heart disease. The invention proves that the six plasma exosome miRNAs can be used as molecular diagnosis markers of the type 2 diabetes mellitus complicated with coronary heart disease, and can be prepared into a kit, a microarray or a biochip for diagnosing the type 2 diabetes mellitus complicated with coronary heart disease, so that the type 2 diabetes mellitus complicated with coronary heart disease can be quickly diagnosed and judged by detecting the expression quantity of the plasma exosome miRNAs of a subject.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis, and specifically relates to exosome miRNA as a molecular marker for diagnosing type 2 diabetes combined with coronary heart disease and its application. Background technique [0002] Coronary heart disease (CHD) plays an important role in cardiovascular system diseases. It is a heart disease caused by narrowing or occlusion of the lumen caused by coronary atherosclerosis, resulting in myocardial ischemia, hypoxia or necrosis. In recent years, the incidence of the disease in the country has been increasing, and it has become an important factor that threatens the health of our people. Diabetes is closely related to the development of coronary heart disease, and the incidence rate of coronary heart disease is higher in people with diabetes. Compared with patients with simple coronary heart disease, their coronary artery lesions are diffuse and multi-vessel lesions, and the stenosis of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/178C12Q2525/207C12Q2531/113C12Q2563/107
Inventor 唐俊楠张金盈张建朝秦臻张增磊徐彦彦路永政
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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