A device used for rapid visualized detection of constant temperature nucleic acid amplification

Abstract

The invention discloses a device used for rapid visualized detection of constant temperature nucleic acid amplification in the technical field of molecular diagnosis. According to the method, constant temperature nucleic acid amplification is adopted to amplify a target nucleic acid, a lateral flow chromatography process is adopted to perform visualized detection, and a whole system is sealed in a transparent box having a size of 9 cm*3.4 cm. Through a special structure design of the device, integration of nucleic acid amplification, sample delivery, development and result determination under a sealed condition is achieved, thus avoiding an uncovering need in detection, avoiding a false positive problem caused by nucleic acid contamination and overcoming the greatest barrier in nucleic acid visualized detection application. The device can achieve high-sensitivity nucleic acid visualized detection on the premise of no generation of nucleic acid contamination, and plays an important role in the fields of disease diagnosis, epidemic disease monitoring, prenatal screening, food safety detection, and the like.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis, and in particular relates to a device for rapid and visual detection of constant temperature nucleic acid amplification. Background technique [0002] As people pay more and more attention to health and the environment, pathogen detection, prenatal diagnosis, genetic disease diagnosis, and food safety detection that are closely related to life and health have become more and more important. Although techniques such as PCR can achieve high-sensitivity detection, they often require sophisticated and expensive instruments, complicated operations, and special laboratory conditions. These limitations limit their use in on-site, real-time monitoring, and community applications, and the nucleic acid visualization diagnostic method can overcome the above problems, and has become a hot spot in molecular diagnostic technology research for a while. Constant temperature nucleic acid amplificatio...

AI Technical Summary

Problems solved by technology

However, so far they have not been widely accepted and used clinically. The most important reason is that it is a non-airtight system. When the nucleic acid is amplified by an order of magnitude, it will cause very serious nucleic acid pollution to the air, resulting in false detection of subsequent sample tests. positive question

In this regard, researchers have also proposed some solutions, such as adding calcein and other blocking agents to the LAMP detection system, but it has been verified that the pollution problem still cannot be completely solved

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