The invention relates to a nested PCR (Polymerase Chain Reaction) detection kit for canna yellow mottle virus and a detection method thereof, which are specially used for detecting the canna yellow mottle virus. The kit comprises an outer-side upstream primer, an outer-side downstream primer, an inner-side upstream primer, an inner-side downstream primer, PCR Buffer, dNTPs, Mgcl2, Taq DNA polymerase, positive control, negative control and ddH2O. According to the invention, two pairs of specific primers are designed according to the gene sequence of the canna yellow mottle virus, extracted DNA is adopted as a template to carry out first-round PCR amplification, first-round PCR products are adopted as a template to carry out second-round PCR amplification, amplification products are detected by gel electrophoresis of agarose, and the detected virus is judged to be the canna yellow mottle virus if specific target fragments with the size being 325bp occur. The kit detects the canna yellow mottle virus by utilizing a nested PCR technology, has the advantages of strong specificity, high sensitivity and short detection time, and can meet the need of entry and exit port quarantine and agricultural production.