Kit for predicting drug resistance of gastrointestinal stromal tumours, and application thereof

Abstract

The invention belongs to the technical field of biochemical analysis, and discloses a kit for predicting the drug resistance of gastrointestinal stromal tumours, and application thereof. The kit comprises a PCR reaction component, a PCR product purification component, a PCR sequencing component sealing plate coating film and a product specification; and an application method of the kit for predicting the drug resistance of the gastrointestinal stromal tumours comprises the following steps of: performing venous blood extraction on a gastrointestinal stromal tumour patient taking the drug; extracting genome DNA by using a phenol chloroform method; setting a reaction system and reaction conditions for PCR amplification, purification and sequencing, and performing PCR sequencing after the reaction is finished; and, analyzing the free tumour cell condition of the gastrointestinal stromal tumour of the gastrointestinal stromal tumour patient taking the drug, so that a conclusion whether thedrug influences the gastrointestinal stromal tumour or not is obtained. The kit provided by the invention is reasonable in design, and can realize accurate acquisition of free tumour cells of the gastrointestinal stromal tumour; therefore, a conclusion whether the drug resistance of the gastrointestinal stromal tumour resists or not is obtained; and furthermore, the kit is convenient to use and low in cost.

Description

technical field [0001] The invention belongs to the technical field of biochemical analysis, and in particular relates to a kit for predicting drug resistance of gastrointestinal stromal tumors and its application. Background technique [0002] Currently, gastrointestinal stromal tumors (Gastrointestinal Stromal Tumors, GIST) are a type of tumor originating from the mesenchymal tissue of the gastrointestinal tract, accounting for the majority of gastrointestinal mesenchymal tumors. Because the tumor is not sensitive to conventional radiotherapy, surgical resection was once the treatment for GIST. The only effective method, for patients who lose the chance of surgery due to tumor progression, metastasis or postoperative recurrence, the median survival period is only 6-18 months, and the 5-year survival rate is less than 10%. Since the beginning of this century, with the widespread application of modern molecular biology techniques in the clinical diagnosis and treatment of GI...

AI Technical Summary

Problems solved by technology

However, there is currently no kit for the prediction of drug resistance of gastrointestinal stromal tumors in the market, which cannot realize the prediction of drug resistance of gastrointestinal stromal tumors, and has no guiding significance for the selection of therapeutic drugs for gastrointestinal stromal tumors.

[0003] Through the above analysis, the existing problems and defects of the prior art are: currently there is no kit for the prediction of drug resistance of gastrointestinal stromal tumors in the market, and it is impossible to realize the prediction of drug resistance of gastrointestinal stromal tumors. The choice of drugs for the treatment of stromal tumors has no guiding significance

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