34 results about "African swine fever virus Antigen" patented technology

The invention provides an African swine fever prevention and / or treatment neutralizing antibody which is an embedded monoclonal antibody and comprises a heavy chain and a light chain. A light chain variable region has a sequence shown as SEQ ID No:6, a heavy chain variable region has a sequence shown as SEQ ID No:2, and both a light chain constant region and a heavy chain constant region come froma swine derived antibody. The invention further provides a gene coding the embedded monoclonal antibody and an expression vector or a transformant comprising the coding gene. The expression carrier can be obtained by inserting the coding gene into pAAV-CAG. The invention further provides a method of utilizing the expression vector to prepare AAV. The antibody sequences or the transformant can beused for preparing detection reagents for African swine fever virus, agents for inducing immunoreaction aiming at African swine fever virus antigen in tested animals or agents for preventing animals from being infected by the African swine fever virus, and rapid and lasting antibody protection such as African swine fever virus vaccines can be provided.

The invention provides an African swine fever virus antibody ELISA (Enzyme Linked Immuno Sorbent Assay) detection kit. A support medium of the kit is coated with an African swine fever virus antigen,and the African swine fever virus antigen is African swine fever virus p30 and / or protein delta p54 protein. The African swine fever virus p30 protein is as shown in SEQ ID No. 1, and the African swine fever virus delta p54 protein is as shown in SEQ ID No. 2. The African swine fever virus antibody ELISA detection kit disclosed by the invention is good in specificity, sensitivity and repeatability, the sensitivity of the kit coated with the two antigens is equivalent to that of indirect immunofluorescence detection, antibody positive can be detected earlier at a low antibody level in the earlystage, a basis is provided for clinical swine herd infection conditions, and the kit plays an important role in preventing and controlling African swine fever virus infection.

The invention discloses an African swine fever virus synthetic peptide ELISA antibody detection kit. The kit includes enzyme-labeled plate, positive control serum, negative control serum, enzyme-labeled secondary antibody, sample diluent, 20 times concentrated washing solution, substrate solution A, substrate solution B, stop solution, wherein the enzyme-labeled plate The polypeptide composition coated with African swine fever virus antigen epitope. The antigen epitope polypeptide composition is one or any combination of the polypeptide shown in sequence 1 in the sequence listing, the polypeptide shown in sequence 2 in the sequence listing, and the polypeptide shown in sequence 3 in the sequence listing . This kit uses chemically synthesized antigen peptides to coat the microtiter plate, with less antigen consumption, high sensitivity and specificity, and can efficiently detect whether there are antibodies infected with African swine fever virus. The kit of the invention has high sensitivity, good specificity, convenient operation and good market prospect.

The invention provides a polypeptide for promoting generation of African swine fever virus antigen specific immune response by a swine body and application thereof, and belongs to the technical field of biological medicines. The polypeptide comprises one or more of a first polypeptide, a second polypeptide, a third polypeptide, a fourth polypeptide and a fifth polypeptide; and the amino acid sequences of the first polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide and the fifth polypeptide are respectively as shown in SEQ ID NO.1-SEQ ID NO.5. The polypeptide can significantly promote ASFV sensitization immune cell proliferation, and can promote a pig body to generate ASFV antigen specific immune response by promoting pig CD4 + T and CD8 + T lymphocytes to secrete IFN-gamma. After animals are immunized, immune response of pig bodies can be obviously promoted. The polypeptide or the polypeptide polymer obtained by polymerizing the polypeptide can be used for preparing a subunit vaccine of the African swine fever virus.

The present invention provides a DNA sequence encoding African swine fever virus antigen, a vector comprising the above DNA sequence, an expression system comprising the above vector, an antigen composition encoded by the above DNA sequence, and the above antigen composition in the detection of African pigs Application of immunological detection method of pestivirus. The present invention optimizes the DNA sequences encoding CP204L, PK205R, PB602L, CP530R, E183L, and PB646L in the main membrane protein and capsid protein of African swine fever virus by eliminating rare codons and other codon optimization methods, and finds that The above-mentioned codon-optimized nucleotide sequence can efficiently express the above-mentioned main membrane protein and capsid protein in a suitable expression system, and the immunological detection method thus established can detect African swine fever quickly, easily and on a large scale Virus.

The invention provides a polypeptide for promoting a swine body to generate African swine fever virus antigen specific immune response and application thereof, and belongs to the technical field of biological medicines. The polypeptide comprises one or more of a first polypeptide, a second polypeptide, a third polypeptide, a fourth polypeptide and a fifth polypeptide; the amino acid sequences of the first polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide and the fifth polypeptide are respectively shown as SEQ ID NO. 1 to SEQ ID NO. 5. The polypeptide provided by the invention can obviously promote ASFV sensitized immune cell proliferation, and can promote the swine body to generate ASFV antigen specific immune response by promoting swine mononuclear macrophages and lymphocytes to secrete IFN-gamma. After animals are immunized, the immune response of the swine body can be obviously promoted. The polypeptide or a polypeptide polymer obtained by polypeptide polymerization can be used for preparing a subunit vaccine of African swine fever virus.

The present invention provides a class of polypeptides for promoting African swine fever virus antigen-specific immune responses in pigs and their application, belonging to the technical field of biomedicine; the polypeptides include a first polypeptide, a second polypeptide, a third polypeptide, One or more of the fourth polypeptide and the fifth polypeptide; the amino acid sequences of the first polypeptide, the second polypeptide, the third polypeptide, the fourth polypeptide and the fifth polypeptide are respectively shown in SEQ ID Shown in NO.1~SEQ ID NO.5. The polypeptide of the present invention can significantly promote the proliferation of ASFV-sensitized immune cells, and can promote porcine CD4 + T and CD8 + T lymphocytes secrete IFN-γ to promote ASFV antigen-specific immune response in pigs. After immunizing animals, it can significantly promote the immune response of pigs. The polypeptide of the present invention or the polypeptide polymer obtained by polymerizing the polypeptide can be used to prepare a subunit vaccine of African swine fever virus.