Recombinant African swine fever antigen cocktail vaccine containing intramolecular adjuvant and application thereof

A African swine fever and cocktail technology, applied in the direction of medical preparations containing active ingredients, virus antigen components, recombinant DNA technology, etc., can solve the problems of side effects, inability to provide immune protection, strong virulence, etc., and achieve easy purification , comprehensive humoral and cellular immune responses, and high expression

Pending Publication Date: 2022-04-22
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous research experience of ASF vaccines showed that: (1) inactivated ASF vaccines cannot provide immune protection; (2) although live attenuated ASF vaccines can provide immune protection against homologous strains, they can cause severe immune side effects, and There is a risk of virulence reversion

Method used

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  • Recombinant African swine fever antigen cocktail vaccine containing intramolecular adjuvant and application thereof
  • Recombinant African swine fever antigen cocktail vaccine containing intramolecular adjuvant and application thereof
  • Recombinant African swine fever antigen cocktail vaccine containing intramolecular adjuvant and application thereof

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Embodiment Construction

[0027] The inventors of the present invention found in their research that the N segment amino acid sequence of OprI-p30-modified p54-TT, OprI-p72 epitope-pE248R-TT and the N segment amino acid sequence of OprI-CD2v were obtained by means of gene fusion and tandem expression -The amino acid sequence of segment C of pEP153R-TTThree kinds of recombinant ASFV antigen fusion proteins, after mixing these three recombinant ASFV antigen fusion proteins in a certain proportion, they are compatible with adjuvants to form a "cocktail" vaccine, and induce the production of neutralizing antibodies after immunizing animals and cellular immune response, indicating that the vaccine form is a promising ASF subunit vaccine. The details of the three recombinant ASFV antigen fusion proteins are as follows:

[0028] (1) Recombinant ASFV antigen fusion protein OPMT, described recombinant protein is fused by OprI, ASFV antigen p30, modified ASFV antigen p54 and TT by connecting peptide (Linker), an...

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Abstract

The invention relates to a recombinant African swine fever antigen'cocktail 'vaccine containing an intramolecular adjuvant and application of the recombinant African swine fever antigen'cocktail' vaccine, and belongs to the field of biotechnological pharmacy, and the'cocktail 'vaccine contains three recombinant African swine fever virus antigen fusion proteins, namely OprI-p30-modified p54-TT, an N-segment amino acid sequence-TT of OprI-p72 epitope-pE248R and an N-segment amino acid sequence of OprI-CD2v and a C-segment amino acid sequence-TT of pEP153R. The invention provides expression and purification methods of the three recombinant African swine fever virus antigen fusion proteins, and component proportions and adjuvant compatibility for preparing the cocktail vaccine. The cocktail vaccine prepared by the invention can stimulate an organism to generate a neutralizing antibody and specific cellular immune response, and can be used as a subunit vaccine for preventing African swine fever.

Description

technical field [0001] The invention belongs to the field of biotechnology and pharmaceuticals, and in particular relates to a recombinant African swine fever antigen "cocktail" vaccine containing an intramolecular adjuvant and an application thereof. Background technique [0002] African swine fever (ASF) is an acute and severe infectious disease of pigs caused by African swine fever virus (ASFV), with a mortality rate of up to 100%. There is no commercial vaccine yet. The World Organization for Animal Health (OIE) lists ASF as an animal disease that must be notified, and my country lists it as a class of animal infectious diseases that must be defended. Past research experience of ASF vaccines shows that: (1) ASF inactivated vaccines cannot provide immune protection; (2) ASF live attenuated vaccines can provide immune protection against homologous strains, but they can cause severe immune side effects, and There is a risk of virulence reversion. (3) Some ASFV antigen sub...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/12A61K39/39C07K19/00C12N15/62A61P31/20
CPCC07K14/005A61K39/12A61K39/39A61P31/20C07K2319/00C12N2710/12022C12N2710/12034A61K2039/70A61K2039/575A61K2039/57
Inventor 邵军军张光磊刘伟杨思成常惠芸
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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