31 results about "Classical swine fever virus antigen" patented technology

The invention discloses a swine fever vaccine heat-resistant freeze-drying protective agent, and a preparation method and an application thereof. The heat-resistant freeze-drying protective agent comprises the following components: trehalose, polyvinylpyrrolidone (PVP), gelatin, sodium glutamate, D-sorbic alcohol, polypeptone, L-arginine, vitamin C and water for injection and is prepared by dissolving and degerming. The heat-resistant freeze-drying protective agent is mixed with swine fever virus antigen liquid according to the volume ratio of 1:(08-1.2) and is frozen and dried after being packaged to obtain swine fever vaccine. The prepared heat-resistant freeze-drying protective agent is simple in formula, is easy to prepare, is suitable for large-scale production and has good protection efficacy on the vaccine. The prepared vaccine has the characteristics of safety and sterility, stable quality, heat resistance and long saving time. The swine fever vaccine heat-resistant freeze-drying protective agent solves the problems that the vaccine is required to be frozen at a low temperature, is not convenient to store and the like in the transportation process. Through the swine fever vaccine heat-resistant freeze-drying protective agent, the swine fever vaccine can be saved for above 30 months at a temperature of 2-8 DEG C.

The invention relates to a swine fever cell-adapted virus, a live swine fever virus vaccine prepared therefrom and a preparation method thereof. In the method, the adaptive virus of classical swine fever virus is used as seedling virus seed, and the original virus seed, basic virus seed and production virus seed of classical classical virus are prepared by successive cell culture, and then the virus seed is produced by the adaptive virus of classical swine fever virus in the passaged cell. After multiplication, the qualified virus liquid is continuously harvested as the antigen for the attenuated swine fever vaccine, and the swine fever cell-adapted live vaccine is obtained through seedling preparation, subpackaging, and freeze-drying. The preparation of poison seeds solves animal welfare issues such as slaughtering rabbits for the prevention of swine fever, and reduces the possible risk of exogenous factors and potential shedding of poison in the preparation of spleen poison. The method for preparing a live vaccine of swine fever virus has the advantages of short virus propagation time, many harvest times, large amount of virus liquid harvested per unit cell, high virus content and low production cost. The prepared swine fever virus live vaccine has good safety and high immune efficacy.

The invention provides a vaccine composition, which contains an immunological amount of classical swine fever virus antigen, an immune amount of Haemophilus parasuis antigen, an immune amount of porcine circovirus type 2 antigen and veterinary acceptable carrier. The vaccine composition can effectively prevent and treat swine fever, porcine circovirus disease and Haemophilus parasuis disease at the same time, and its single injection can achieve the immune effect of single vaccine injection twice, with small side effects, long immunization period, and time-consuming Less, less effort.

A recombinant vector for transforming a plant, a plant transformed with the recombinant vector, a plant-made classical swine fever virus antigen pmE2 protein expressed in the plant and uses thereof is provided. By using a recombinant vector having a polynucleotide encoding a GP55 protein of CSFV according to the present invention; and a polynucleotide encoding a cellulose-binding domain protein; and a plant transformed with the recombinant vector, a plant-made classical swine fever virus antigen pmE2 protein may be produced with high efficiency, and has higher safety and stability than those achieved by other production methods. Also, since the plant-derived classical swine fever virus antigen protein pmE2 has a cellulose-binding domain (CBD) protein, it may be usefully used as an effective marker to determine a virus exposure pathway and an antibody producing pathway.

The invention relates to a bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof. According to the method, a porcine circovirus 2 subunit antigen and a swine fever virus antigen are mixed and lyophilized to prepare the bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection; or a porcine circovirus 2 totivirus antigen inactivated vaccine is used as a diluent to dilute a swine fever virus vaccine, so as to prepare a bivalent vaccine composition kit for resisting swine fever virus and porcine circovirus 2 infection. The bivalent vaccine composition does not produce immunity interference; after immunization, the swine fever virus antigen speeds up in immunoreaction; the protective antibodies provided by the vaccine composition reach an immunization protective dose one week earlier than a single vaccine antibody; and the antibodies produced by the porcine circovirus vaccine in the bivalent vaccine have similar effects as that produced by single vaccine immune. The method has the advantages of simple vaccine production process and high production efficiency. The animal experiments prove that the effect of the vaccine composition is better that that of single vaccine immune, therefore the invention has great application value.

The invention provides a vaccine composition, which comprises: immunizing amount of classical swine fever virus antigen, immunizing amount of porcine pseudorabies virus antigen and immunizing amount of Haemophilus parasuis antigen. The vaccine composition is safe to use, has controllable quality, good immune effect, and the protection rate of the virus challenge reaches 80-100%. Satisfactory immune effect can be achieved by intranasally immunizing 0-3 day-old piglets and intramuscularly immunizing 50-day-old piglets.

The invention provides a vaccine composition. The vaccine composition comprises hog cholera virus antigen and porcine reproductive and respiratory syndrome (PRRS) antigen, wherein the hog cholera virus antigen is a hog cholera rabbit attenuated strain, the PRRS antigen is a high-pathogenicity PRRS virus, and the content ratio of the PRRS antigen and the hog cholera virus antigen is 100:1-100:10. The vaccine composition can eliminate the interference between the two types of antigen, achieves the purpose of preventing two infectious diseases by one injection, and greatly reduces immunization times and animal stress response.