31 results about "Classical swine fever virus antigen" patented technology

The invention relates to a swine fever cell-adapted virus, a live swine fever virus vaccine prepared therefrom and a preparation method thereof. In the method, the adaptive virus of classical swine fever virus is used as seedling virus seed, and the original virus seed, basic virus seed and production virus seed of classical classical virus are prepared by successive cell culture, and then the virus seed is produced by the adaptive virus of classical swine fever virus in the passaged cell. After multiplication, the qualified virus liquid is continuously harvested as the antigen for the attenuated swine fever vaccine, and the swine fever cell-adapted live vaccine is obtained through seedling preparation, subpackaging, and freeze-drying. The preparation of poison seeds solves animal welfare issues such as slaughtering rabbits for the prevention of swine fever, and reduces the possible risk of exogenous factors and potential shedding of poison in the preparation of spleen poison. The method for preparing a live vaccine of swine fever virus has the advantages of short virus propagation time, many harvest times, large amount of virus liquid harvested per unit cell, high virus content and low production cost. The prepared swine fever virus live vaccine has good safety and high immune efficacy.

The invention provides a vaccine composition, which contains an immunological amount of classical swine fever virus antigen, an immune amount of Haemophilus parasuis antigen, an immune amount of porcine circovirus type 2 antigen and veterinary acceptable carrier. The vaccine composition can effectively prevent and treat swine fever, porcine circovirus disease and Haemophilus parasuis disease at the same time, and its single injection can achieve the immune effect of single vaccine injection twice, with small side effects, long immunization period, and time-consuming Less, less effort.

The invention relates to a bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof. According to the method, a porcine circovirus 2 subunit antigen and a swine fever virus antigen are mixed and lyophilized to prepare the bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection; or a porcine circovirus 2 totivirus antigen inactivated vaccine is used as a diluent to dilute a swine fever virus vaccine, so as to prepare a bivalent vaccine composition kit for resisting swine fever virus and porcine circovirus 2 infection. The bivalent vaccine composition does not produce immunity interference; after immunization, the swine fever virus antigen speeds up in immunoreaction; the protective antibodies provided by the vaccine composition reach an immunization protective dose one week earlier than a single vaccine antibody; and the antibodies produced by the porcine circovirus vaccine in the bivalent vaccine have similar effects as that produced by single vaccine immune. The method has the advantages of simple vaccine production process and high production efficiency. The animal experiments prove that the effect of the vaccine composition is better that that of single vaccine immune, therefore the invention has great application value.