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Vaccine composition against swine fever virus and porcine circovirus infection and its preparation and application

A porcine circovirus and swine fever virus technology, applied in antiviral agents, virus antigen components, medical preparations containing active ingredients, etc., can solve the problems of cumbersome seed virus preparation process, reduced immune effect, and large batch-to-batch differences , to avoid immune stress response and reduce the immune effect, simplify the production process, and achieve the effect of high titer content

Active Publication Date: 2015-08-19
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of this method is cumbersome, the controllability is poor, the difference between batches is large, the production cycle is long, and it can only prevent and treat one disease
[0007] Therefore, the problem that exists at present is to need research and development a kind of production process is simple, the vaccine quality is good, can prevent and treat swine fever, porcine circovirus disease two kinds of infectious diseases simultaneously, and can avoid multiple inoculations and cause immune stress response and immune effect Reduced vaccine and method for its preparation

Method used

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  • Vaccine composition against swine fever virus and porcine circovirus infection and its preparation and application
  • Vaccine composition against swine fever virus and porcine circovirus infection and its preparation and application
  • Vaccine composition against swine fever virus and porcine circovirus infection and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] 1) preparation of classical swine fever virus antigen;

[0037] 2) Preparation of porcine circovirus type 2 subunit antigen;

[0038] 3) Antigens of classical swine fever virus and subunit antigens of porcine circovirus type 2 are mixed in proportion and then freeze-dried to prepare a dual vaccine composition against infection of classical swine fever virus and porcine circovirus type 2;

[0039] 4) Efficacy evaluation was carried out after immunizing animals with the dual vaccine composition against classical swine fever virus and porcine circovirus type 2 infection and the two single vaccines respectively.

[0040] Scheme 2 is to use the prepared porcine circovirus type 2 whole virus antigen inactivated vaccine as a diluent to dilute the swine fever virus vaccine to obtain a dual vaccine composition resistant to swine fever virus and porcine circovirus type 2 infection. Prepare the double combination vaccine composition test kit of anti-swine fever virus and porcine ...

Embodiment 1

[0050] Example 1: Lyophilize after mixing CSFV and porcine circovirus type 2 subunit antigens to prepare a dual vaccine composition against CSFV and porcine circovirus type 2 infection

[0051] 1. Preparation of CSFV antigen solution

[0052] (1) The highly sensitive ST cells (purchased from ATCC) (American type culture collection, American type culture collection) grown into a good monolayer were treated with 0.125wt% trypsin and 0.03wt% EDTA ( ethylenediaminetetraacetic acid) digestion liquid was digested and dispersed, counted cells, and inoculated with a suitable density, added MEM containing 3wt% to 5wt% FBS (fetal bovine serum) (purchased from PAA company, batch number A15110-1462) (purchased from GIBCO Company, batch number 856833), and at the same time add seed virus according to M.O.I. (multiplicity of infection) = 0.1-0.2 exposure dose, and place in a 34-37°C incubator for cultivation.

[0053] (2) After culturing for three days, the first poisoning was carried out,...

Embodiment 2

[0089] Embodiment 2: take the prepared porcine circovirus type 2 whole virus antigen inactivated vaccine as the diluent, dilute the mode of the dual vaccine composition of swine fever virus vaccine and obtain anti-swine fever virus and porcine circovirus type 2 infection To prepare a dual vaccine composition kit against classical swine fever virus and porcine circovirus type 2 infection.

[0090] 1. Preparation of CSFV antigen solution

[0091] (1) Digest and disperse ST cells (derived from ATCC) that have grown into a good monolayer and are highly sensitive to classical swine fever virus with a digestive solution of 0.125% trypsin and 0.03% EDTA, count the cells and inoculate them at an appropriate density , add 3wt% ~ 5wt% FBS (purchased from PAA company, batch number A15110-1462) MEM (purchased from GIBCO, batch number 856833), at the same time add seed poison according to M.O.I. cultured in an incubator.

[0092] (2) After culturing for three days, the first poisoning wa...

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Abstract

The invention relates to a bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection, and preparation and application thereof. According to the method, a porcine circovirus 2 subunit antigen and a swine fever virus antigen are mixed and lyophilized to prepare the bivalent vaccine composition for resisting swine fever virus and porcine circovirus 2 infection; or a porcine circovirus 2 totivirus antigen inactivated vaccine is used as a diluent to dilute a swine fever virus vaccine, so as to prepare a bivalent vaccine composition kit for resisting swine fever virus and porcine circovirus 2 infection. The bivalent vaccine composition does not produce immunity interference; after immunization, the swine fever virus antigen speeds up in immunoreaction; the protective antibodies provided by the vaccine composition reach an immunization protective dose one week earlier than a single vaccine antibody; and the antibodies produced by the porcine circovirus vaccine in the bivalent vaccine have similar effects as that produced by single vaccine immune. The method has the advantages of simple vaccine production process and high production efficiency. The animal experiments prove that the effect of the vaccine composition is better that that of single vaccine immune, therefore the invention has great application value.

Description

technical field [0001] The invention belongs to the technical field of animal biopharmaceuticals, and relates to a vaccine composition for pigs, in particular to a dual vaccine composition against classical swine fever virus and porcine circovirus type 2 infection and its preparation and application. Background technique [0002] Classical swine fever (CSF) is a devastating infectious disease caused by classical swine fever virus (CSFV), which seriously endangers the swine industry. The mortality rate of this disease is as high as 80% to 90%. The World Organization for Animal Health (OIE) lists it as an OIE disease list, and my country also lists it as a first-class infectious disease. The prevention and control of swine fever has always been a subject that the countries with swine fever in the world attach great importance to. For this reason, all countries have invested a lot of manpower and material resources and achieved great results. Vaccination is still an important ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/20A61K39/12A61K39/187
Inventor 李向东廖永洪张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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