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A kind of vaccine composition and its preparation method and application

A technology of vaccine composition and antigen, which is applied in the field of vaccine composition, can solve the problems of triple combination vaccine of Haemophilus parasuis that does not yet exist, increase strength, and stress response of pig herds, so as to reduce costs and reduce vaccinations The number of times, the effect of enhancing the immune effect

Active Publication Date: 2017-09-12
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of the three vaccines requires secondary immunization, which increases the intensity of the work, and multiple immunizations are also likely to cause stress reactions in pigs
[0006] There is currently no triple combination vaccine for classical swine fever, porcine circovirus, and Haemophilus parasuis

Method used

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  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 classical swine fever antigen, porcine circovirus antigen and Haemophilus parasuis antigen

[0040] 1 Preparation of swine fever antigen

[0041] 1.1 The ST cells (purchased from ATCC) that are highly sensitive to classical swine fever virus (the attenuated strain of classical swine fever rabinized by the China Veterinary Drug Administration, preservation number AV1412) grown into a good monolayer were treated with 0.125% trypsin and 0.03% Digest and disperse the EDTA digestion solution, inoculate the cell culture bottle after counting the cells, add 1.5%-5% FBS MEM cell culture medium, and add seed poison at the same time according to M.O.I. M.O.I.=0.1-0.6, more preferably the inoculated dose is M.O.I.=0.2-0.4, placed in a 34-37°C incubator for cultivation, further preferably the cultivation temperature is 34-35°C.

[0042] After three days of cultivation, the first time of poisoning is carried out. After the poisoning, the cell mainten...

Embodiment 2

[0059] The preparation of embodiment 2 swine fever, porcine circular ring and Haemophilus parasuis triple mixed vaccine composition

[0060] Preparation of 1 para-Hemophilus suis and porcine circular double inactivated vaccine

[0061] 1.1 Preparation of preservatives

[0062] 1% (w / v) thimerosal aqueous solution: 1g of thimerosal is dissolved in 100ml of purified water, and autoclaved at 121°C for 30 minutes for later use.

[0063] 1.2 Preparation of diluent

[0064] Sterile PBS buffer solution: Dissolve 8g sodium chloride, 0.25g potassium chloride, 3.63g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate in 900ml purified water, then dilute to 1L, autoclave at 121°C for 30min spare.

[0065] 1.3 Vaccine adjuvant treatment

[0066] Sterilization of the Gel01 adjuvant: transfer the Gel01 adjuvant into a sterilizable container, and autoclave at 121° C. for 30 minutes for later use.

[0067] 1.4 Matching seedlings

[0068] Through aseptic operation, the conc...

Embodiment 3

[0076] Example 3 Different antigen content swine fever, porcine circular and Haemophilus parasuis triple mixed vaccine efficacy test

[0077] 1 test material

[0078] Vaccine 1 (Haemophilus parasuis 10) prepared in embodiment 2 7 CFU / capacity, swine fever virus 10 4.0 TCID 50 / touquan, porcine circovirus 10 4.0 TCID 50 / head), vaccine 2 (Haemophilus parasuis 10 8 CFU / capacity, swine fever virus 10 5.0 TCID 50 / touquan, porcine circovirus 10 5.0 TCID 50 / head), vaccine 3 (Haemophilus parasuis 10 9 CFU / capacity, swine fever virus 10 6.0 TCID 50 / touquan, porcine circovirus 10 6.0 TCID 50 / head), vaccine 4 (Haemophilus parasuis 10 10 CFU / capacity, swine fever virus 10 6.0 TCID 50 / touquan, porcine circovirus 10 6.0 TCID 50 per head) and vaccine 5 (Haemophilus parasuis 2×10 10 CFU / capacity, swine fever virus 10 6.0 TCID 50 / touquan, porcine circovirus 10 6.0 TCID 50 / toufen).

[0079] 3-4 week-old weaned piglets free of antibodies to CSF, Circus porcine,...

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Abstract

The invention provides a vaccine composition, which contains an immunological amount of classical swine fever virus antigen, an immune amount of Haemophilus parasuis antigen, an immune amount of porcine circovirus type 2 antigen and veterinary acceptable carrier. The vaccine composition can effectively prevent and treat swine fever, porcine circovirus disease and Haemophilus parasuis disease at the same time, and its single injection can achieve the immune effect of single vaccine injection twice, with small side effects, long immunization period, and time-consuming Less, less effort.

Description

technical field [0001] The present invention relates to a vaccine composition. Background technique [0002] Classical swine fever is an acute, febrile, highly contagious infectious disease of pigs, characterized by high temperature and microvascular degeneration, which can cause systemic hemorrhage, necrosis, and infarction. In the early years, it was also known as hog cholera, which was extremely harmful to pigs and caused heavy losses to the pig industry. [0003] Porcine circovirus (abbreviation: PCV) is the smallest animal virus found so far. PCV is known to have two serotypes, PCV1 and PCV2. PCV1 is a non-pathogenic virus. PCV2 is a pathogenic virus. It is the main pathogen of Postweaning Multisystemic Wasting Syndrome (PMWS) in weaned piglets. It was first discovered in Canada (1991), and soon occurred and spread in some countries in Europe, America and Asia, including my country. , in addition to PMWS, PDNS (porcine dermatitis and nephrotic syndrome), PNP (prolife...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/14A61P31/20
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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