Multicolor high-risk HPV (human papilloma virus) fluorescence detection kit

Abstract

The invention discloses a multicolor high-risk HPV (human papilloma virus) fluorescence detection kit which comprises a warm-boot Taq enzyme system, universal primers, fluorescent probes, a negative control and a positive control, wherein the fluorescent probes are HPV-specific MGB probes. The kit is suitable for qualitatively detecting 18 high-risk HPV genotypes capable of causing cervical carcinoma in cervix exfoliated cell and genitourinary tract secretion samples. The kit uses the multiplex fluorescence PCR (polymerase chain reaction) technique to implement detection of 18 HPV genotypes in one tube amplification system. The kit can simultaneously detect 15 common high-risk HPV genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 and 82) and can also detect 3 rare high-risk HPV genotypes (26, 53 and 73). The detection kit has the advantage of high detection flux and greatly lowers the detection cost.

Description

technical field [0001] The invention relates to a HPV fluorescence detection kit, in particular to a multi-color high-risk HPV fluorescence detection kit. Background technique [0002] Cervical cancer is the most common gynecological malignancy. The high-incidence age of carcinoma in situ is 30-35 years old, and that of invasive carcinoma is 45-55 years old. In recent years, the onset tends to be younger. Early detection and treatment of cervical cancer is the key to effective treatment of cervical cancer. [0003] Existing studies have shown that the main cause of cervical cancer is persistent or recurrent infection of high-risk human papillomavirus (HPV). More than 90% of cervical cancers are associated with high-risk HPV infection, and persistent high-risk HPV infection is the main risk factor for cervical cancer. Therefore, HPV DNA testing has become an important part of cervical cancer screening and health management in many countries around the world. There are man...

AI Technical Summary

Problems solved by technology

[0004] The disadvantages of the existing techniques for genotyping detection of high-risk human papillomaviruses by using fluorescent PCR method are mainly low amplification sensitivity and long time-consuming

In the early stage, it is generally necessary to go through tedious nucleic acid extraction steps, and then use the common Taqman probe method for multiplex PCR amplification, and generally use FAM and HEX two-channel detection, which cannot specifically classify high-risk HPV types, and is difficult to meet the needs of actual use.

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