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Method and kit for detecting HPV (Human Papillomavirus) genotype

A technology of genotype and kit, which is applied in the field of detection of HPV genotype, method and kit, can solve the problems of complex operation process of detection technology and achieve the effect of low detection cost

Active Publication Date: 2013-10-23
HANGZHOU DIAGENS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method and kit for detecting HPV genotypes, aiming at solving the problem of complicated operation process of the existing detection technology

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  • Method and kit for detecting HPV (Human Papillomavirus) genotype
  • Method and kit for detecting HPV (Human Papillomavirus) genotype
  • Method and kit for detecting HPV (Human Papillomavirus) genotype

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Embodiment Construction

[0033] The present invention provides a method and a kit for detecting HPV genotypes. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0034] In order to make the detection method low in price and simple and easy to operate, a method for detecting HPV genotype provided by the present invention only needs to use one or two restriction endonucleases to carry out fragment length polymorphism in the detection process Sex map analysis can achieve the purpose of determining the HPV type. The method of the present invention can detect all 13 high-risk types of HPV and 19 common low-risk types of HPV, wherein the 13 high-risk types of HR-HPV include HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68; 20 ...

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Abstract

The invention discloses a method and a kit for detecting an HPV (Human Papillomavirus) genotype. The method for detecting the HPV genotype comprises the following steps of: A, extracting DNA (Deoxyribonucleic Acid); b, carrying out PCR (Polymerase Chain Reaction) on a DNA template, and amplifying to obtain a PCR product of an HPV-infected DNA sample; and C, carrying out restricted segment length polymorphism analysis through restriction endonuclease. The method is low in detection cost and can be used for rapidly and intuitively determining the type of the infected HPV according to a special HPV type atlas.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and a kit for detecting HPV genotypes. Background technique [0002] Cervical cancer is one of the most common cancers in women worldwide. There are approximately 500,000 cases of cervical cancer each year, of which nearly 275,000 patients die from the cancer. In 1976, German scholar zur Hausen discovered that human papilloma virus (HPV) plays a very important role in the pathogenesis of cervical cancer. Studies show that about 70 percent of HPV infections clear up on their own within 7 to 12 months of initial infection with the virus, and 90 percent clear within 2 years. The remaining 10% of HPV infections will develop into persistent infections. Persistent infection with a high-risk HPV genotype (HR-HPV) is a key risk factor in the development of cervical cancer. The onset of the cancer often lasts for as long as 10 years. Therefore, early detection of HPV genotype, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 陈玲瀚宋宁
Owner HANGZHOU DIAGENS BIOTECH CO LTD
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