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Q-Orbitrap high-resolution mass spectrometric detection method for illegal additives in weight-losing type Chinese patent medicines and health foods

A technology of high-resolution mass spectrometry and health food, which is applied in the direction of measuring devices, material separation, and analysis of materials, to achieve the effects of high sensitivity, strong confirmation, and simple and quick operation

Inactive Publication Date: 2015-05-06
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the powerful qualitative and quantitative potential of Orbitrap HRMS has not been used to detect illegal additions in weight-loss Chinese patent medicines and health foods

Method used

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  • Q-Orbitrap high-resolution mass spectrometric detection method for illegal additives in weight-losing type Chinese patent medicines and health foods
  • Q-Orbitrap high-resolution mass spectrometric detection method for illegal additives in weight-losing type Chinese patent medicines and health foods
  • Q-Orbitrap high-resolution mass spectrometric detection method for illegal additives in weight-losing type Chinese patent medicines and health foods

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Experimental program
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Effect test

Embodiment

[0022] 1 Experimental materials

[0023] 1.1 Instruments and reagents

[0024] UHPLC–Q–Orbitrap Ultra Performance Liquid Chromatography–Mass Spectrometry System, including Accela 1250LC pump and autosampler, Q Exactive TM Mass spectrometry part (Thermo Fisher Scientific, Germany). XCalibur 2.2 software (Thermo Fisher Scientific, MA, USA) was used for instrument control and data processing; Q Exactive 2.1 software (Thermo Fisher Scientific, MA, USA) was used to control the mass spectrometer. The column is Hypersil GOLD aQ C 18 column (100 mm×2.1 mm, 1.9 μm) (Thermo Fisher Scientific, USA). KQ-300GDV temperature-controlled ultrasonic instrument (Kunshan, China). Chromatographically pure methanol, acetic acid, and ammonium acetate were purchased from TEDIA (USA), and ultrapure water (18.2 MΩ) was produced by a Milli–Q Advantage A10 ultrapure water system.

[0025] Reference substances: caffeine, batch number 171215-201211, content 99.9%; furosemide, batch number 100544-20110...

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Abstract

The invention provides a detection method for illegal additives in weight-losing type Chinese patent medicines and health foods. A Q-Orbitrap high-resolution mass spectrometer is adopted and a Full MS Scan / dd-MS2 (Top N) scanning mode is carried out so that positive and negative ions are detected at the same time; the separation, high-precision first-grade mass spectrometric scanning and high-precision second-grade mass spectrometric scanning of analytes in a sample can be finished in an analyzing period to obtain accurate mass quantity and accurate fragment ion information; chromatography retention time, a first-grade mass spectrum and a second-grade mass spectrum of the sample are compared with those of a weight-losing type chemical component comparison product to determine that whether weight-losing type chemical medicines are doped into the sample or not; and rapid screening, confirmation and quantification can be carried out on a potential positive sample. The detection method has the advantages of high sensitivity, good selectivity, strong confirmation, simplicity and rapidness of operation and the like, can be used for accurately screening, has the sensitivity beyond comparison of traditional HPLC and low-resolution LC-MS, and can be used for identifying, confirming and quantitatively analyzing compounds in a single analysis period (12 minutes).

Description

technical field [0001] The invention relates to the technical field of analysis of medicines, health food or food, in particular to a Q-Orbitrap high-resolution mass spectrometry detection method for illegal additives in weight-loss Chinese patent medicines and health food. Background technique [0002] In recent years, the illegal addition of chemical drugs in Chinese patent medicine health food has become a common phenomenon. Some unscrupulous manufacturers illegally add chemical drugs to Chinese herbal medicine and health food in order to one-sidedly pursue the therapeutic effect and obtain huge economic profits. Caffeine, furosemide, phenolphthalein, sibutramine hydrochloride, fenfluramine hydrochloride and other drugs are commonly used illegal additives in weight loss products, all of which have certain adverse reactions, such as abuse or improper use can lead to adverse consequences. Traditional Chinese medicine and pure natural health food have been loved by the major...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 谭会洁石峰郭常川李军巩丽萍
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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