Micro-fluidic chip for researching growth and reproduction of algae

A microfluidic chip and algae technology, which is applied in the field of microfluidic chips for studying the growth and reproduction of algae, can solve the problems of uneven diffusion of nutrient solution, accumulation of harmful metabolites, etc. Automatic real-time monitoring of recording effects

Pending Publication Date: 2018-09-11
FUDAN UNIV
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Problems solved by technology

[0007] The microfluidic chip used to study the growth and reproduction of algae provided by the present invention uses optically transparent materials as the base material, adopts a microfluidic continuous perfusion system, realizes the automatic separation of algae germ cells from the microscopic level, and monitors algae cells in real time. A series of growth and reproductive changes, which solves the problems of uneven diffusion in nutrient solution and accumulation of harmful metabolites in traditional research methods

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  • Micro-fluidic chip for researching growth and reproduction of algae
  • Micro-fluidic chip for researching growth and reproduction of algae

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Embodiment Construction

[0032] (1) Substrate preparation: The single crystal silicon wafer was cleaned in Piranha solution (98% concentrated sulfuric acid: 30% hydrogen peroxide) for 15 minutes to deoxidize, rinsed with deionized water, dried with nitrogen, and baked at 200 ° C for 30 minutes.

[0033] (2) Spin the glue: put the prepared substrate on the spin coating position of the high-pressure adsorption spin coating machine (Spin-Coater KW-4A, ChematTechnology, Inc.), pour AZ 50XT series glue on the center of the substrate, and rotate at 2000r / min for 2min , let it stand for 10 minutes to reduce the air bubbles on the surface of the silicon wafer.

[0034] (3) Pre-baking: Place the glue-coated silicon wafer on a heating plate at 65°C, 90°C, and 65°C for 2 minutes, 7 minutes, and 2 minutes.

[0035] (4) Exposure: Place the mask of the sample pipeline layer and the valve control layer on the spin-coated substrate, and expose it with a UV exposure machine.

[0036] (5) Development: The development ...

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Abstract

The invention belongs to the technical field of growth and reproduction of algae and in particular relates to a micro-fluidic chip for researching the growth and reproduction of the algae. The micro-fluidic chip provided by the invention is composed of a sample channel layer, a valve control layer and a substrate layer, wherein the sample channel layer comprises four parts which are series connected in sequence: a sample inlet, an alga growth observation chamber, a reproduction cell capturing chamber and a sample outlet; alga culture, reproduction cell releasing and automatic separation functions are realized by adopting a continuous pouring technology, and alga cells and sub-cell structures can be observed and recorded for long time in real time; a culture medium of the algae can be rapidly and accurately replaced, and influences, caused by manual intervention, on a growth state of the algae are avoided; raw cells, which are automatically separated, also can be independently cultured.The chip provided by the invention is especially suitable for reproduction and growth researches of various large-size algae. The chip is low in production cost and a method is simple, easy to operate and convenient to popularize.

Description

technical field [0001] The invention belongs to the technical field of algae growth and reproduction, and in particular relates to a microfluidic chip for studying the growth and reproduction of algae. Background technique [0002] In recent years, after the eutrophication of water bodies, large-scale algae outbreaks such as green tides and red tides have occurred frequently, which has become a global problem. The green tide caused by the overgrowth of large algae. When the green tide is severe, the entire sea near the coast is covered by algae, which consumes oxygen in the water and causes the death of zooplankton and plants in the water body. and may form swamps, producing H 2 S gas has caused serious damage to the ecosystem of the entire ocean. On the other hand, the green tide will also affect people's near-shore activities, damage the aquaculture industry, and affect shipping traffic. Therefore, it is very important to study the mechanism of algal blooms to solve the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N1/12C12R1/89
CPCC12M23/16C12M29/06C12N1/12
Inventor 李晓旭徐智轩张新联董恒赵望刘琪柯少瑞刘思秀隋国栋
Owner FUDAN UNIV
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