Optical projection tomography image capturing method based on depth-of-field fusion

A technology for optical projection tomography and image acquisition, which is applied in the direction of material analysis, scientific instruments, and measuring devices by optical means, which can solve the problem of impossible to focus clearly and achieve the effect of improving resolution.

Active Publication Date: 2013-09-18
INST OF AUTOMATION CHINESE ACAD OF SCI
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Problems solved by technology

Only those structures that are at or near the plane of focus are visible, making it impossible for even the simplest structures with relatively flat 3D depths to be fully in focus in a single image
In optical projection tomography, the thickness of the sample is generally 2-3 mm, and the problem of depth of field becomes more serious when the magnification is high.
When...

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  • Optical projection tomography image capturing method based on depth-of-field fusion
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  • Optical projection tomography image capturing method based on depth-of-field fusion

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Embodiment Construction

[0011] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. While illustrations of parameters containing particular values ​​may be provided herein, it should be understood that the parameters need not be exactly equal to the respective values, but rather approximate the values ​​within acceptable error margins or design constraints.

[0012] The invention discloses an optical projection tomography image acquisition method based on depth of field fusion. The present invention is specially aimed at performing depth-of-field fusion on images of different focal positions in optical projection tomography at a certain fixed rotation angle, and selects the pixel value of the clearest point of each pixel position on the image sequence as the gray value of the pixel on the synthesized image....

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Abstract

The invention discloses an optical projection tomography image capturing method based on depth-of-field fusion. The method comprises the following steps: step one, moving the relative position of a sample and a microscopic device under the same rotation angle, acquiring two-dimensional image sequences on different focusing positions; step two, selecting the clearest points of pixel positions in the two-dimensional image sequence, synthesizing to generate a panorama deep image. The different focusing position images acquired by optical projection tomography system can be fused through the method disclosed by the invention, the obtained panorama deep image is used for three-dimensional reconstruction, and the resolution of the three-dimensional image is improved.

Description

technical field [0001] The invention relates to the technical field of optical projection tomography, in particular to an image acquisition method of optical projection tomography based on depth-of-field fusion. Background technique [0002] Optical projection tomography (Optical Projection Tomography, OPT) technology can realize structural and molecular specific imaging of 1-10 mm scale biological samples. In the OPT imaging process, the sample is relatively thin and relatively transparent, and the scattering effect is negligible when visible light passes through the sample, and the photon is mainly absorbed by the sample, so it can be considered that the light propagates through the sample along a straight line. When OPT scans, the sample is fixed on a high-precision rotary table, and a highly stable LED or laser is used as the irradiation source to irradiate the sample. The optical signal passing through the sample is amplified by a microscopic device and then transmitted...

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Application Information

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IPC IPC(8): G01N21/17
Inventor 田捷郭进董迪杨鑫马喜波杨玉洁
Owner INST OF AUTOMATION CHINESE ACAD OF SCI
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