Optical projection tomography image capturing method based on depth-of-field fusion

Abstract

The invention discloses an optical projection tomography image capturing method based on depth-of-field fusion. The method comprises the following steps: step one, moving the relative position of a sample and a microscopic device under the same rotation angle, acquiring two-dimensional image sequences on different focusing positions; step two, selecting the clearest points of pixel positions in the two-dimensional image sequence, synthesizing to generate a panorama deep image. The different focusing position images acquired by optical projection tomography system can be fused through the method disclosed by the invention, the obtained panorama deep image is used for three-dimensional reconstruction, and the resolution of the three-dimensional image is improved.

Description

technical field [0001] The invention relates to the technical field of optical projection tomography, in particular to an image acquisition method of optical projection tomography based on depth-of-field fusion. Background technique [0002] Optical projection tomography (Optical Projection Tomography, OPT) technology can realize structural and molecular specific imaging of 1-10 mm scale biological samples. In the OPT imaging process, the sample is relatively thin and relatively transparent, and the scattering effect is negligible when visible light passes through the sample, and the photon is mainly absorbed by the sample, so it can be considered that the light propagates through the sample along a straight line. When OPT scans, the sample is fixed on a high-precision rotary table, and a highly stable LED or laser is used as the irradiation source to irradiate the sample. The optical signal passing through the sample is amplified by a microscopic device and then transmitted...

AI Technical Summary

Problems solved by technology

Only those structures that are at or near the plane of focus are visible, making it impossible for even the simplest structures with relatively flat 3D depths to be fully in focus in a single image

In optical projection tomography, the thickness of the sample is generally 2-3 mm, and the problem of depth of field becomes more serious when the magnification is high.

When observing samples, microscope imaging is required to have both higher resolution and sufficient depth of field, which is a contradiction of traditional optical hardware

However, for optical projection tomography, this is a problem that has to be solved, so the depth of field problem is a bottleneck that restricts optical projection tomography to obtain high-resolution images

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