Optical projection tomography method capable of automatically processing depth of field

Abstract

The invention relates to an optical projection tomography method capable of automatically processing depth of field. The optical projection tomography method comprises the following steps of radially moving a position of a sample at a rotation center, and collecting a series of projection drawings of the sample at a same angle under different focal lengths; and selecting clear projection drawings from the series of the projection drawings of the sample according to a mean square deviation value for carrying out three-dimensional image reconstruction. According to the optical projection tomography method capable of automatically processing the depth of field, disclosed by the invention, the automatic process of the depth of field of an optical projection tomography can be quickly realized, clear two-dimensional projection images are selected to be used for carrying out three-dimensional reconstruction, and the resolution ratio of a reconstruction body is improved.

Description

technical field [0001] The invention relates to an optical projection tomography technology, in particular to an optical projection tomography method for automatically processing depth of field. Background technique [0002] Optical projection tomography (OPT) technology can realize structural and molecular-specific imaging of biological samples at a scale of 1-10 mm. It has many advantages such as high resolution, integration of structure and function, no radiation, and low cost. Live organisms conduct qualitative and quantitative research at the cellular level to achieve real-time, non-invasive, dynamic, and in vivo imaging of organisms. At present, OPT technology has been applied to the imaging of small animal embryos and complete organs such as mice, and the imaging of model animals such as Drosophila. OPT imaging of model animals such as mice and Drosophila is of great significance for the study of gene expression, protein interaction, and preclinical drug development....

AI Technical Summary

Problems solved by technology

[0004] Optical projection tomography belongs to microscopic imaging, with a small imaging field of view and high magnification, resulting in a very limited depth of field in the object space

In addition, the radial error of the mechanical device, the rotational movement of the sample, etc. may cause the image that is clearly focused at the beginning to become blurred after a certain angle of rotation

So the depth of field problem is a bottleneck that plagues optical projection tomography to obtain high-resolution images

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