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Microfluidic chip applicable to medicine screening of zebra fish embryos

A zebrafish embryo and microfluidic chip technology, which is applied in the determination/inspection of microorganisms, bioreactors/fermenters for specific purposes, biomass post-processing, etc., can solve the problems of labor-intensive, high consumption, and low degree of automation.

Inactive Publication Date: 2012-10-31
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a microfluidic chip and a microfluidic liquid operating system for the problems of low automation, labor-intensive, and high consumption of zebrafish embryo fluid replacement in the prior art, and the system can realize zebrafish embryo drug screening In the process, the whole process of liquid exchange is completed at the set time point. In a preferred embodiment, in a chip of 4 * 6 "fish farming micro-pools" (hereinafter referred to as micro-pools), each micro-pool There is only one fish embryo, and these fish microponds are connected to the fluid layer pipelines, and the fluid layer pipelines control the dosing or elution of the liquid in each micropool at a set time point through program control, thereby realizing automatic liquid exchange process

Method used

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  • Microfluidic chip applicable to medicine screening of zebra fish embryos
  • Microfluidic chip applicable to medicine screening of zebra fish embryos
  • Microfluidic chip applicable to medicine screening of zebra fish embryos

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Structure of the microfluidic chip. This embodiment describes in detail a preferred structure of the microfluidic chip of the present invention, the structure is as figure 1 shown. The input microfluidic channel opening above the microfluidic chip is bifurcated to form 3 branches, and each branch is further bifurcated to form 2 small branches. Connected, each branch is further bifurcated into 4 small branches to form 4 openings on the inner wall of each micro pool, and the 4 openings are equally spaced on the inner wall of the micro pool, making the injection and replacement of liquid more efficient; output micro flow There are 6 channels in total, each of which communicates with a row of micro-cells. Similarly, the micro-flow channels form 4 openings on the inner wall of each micro-cell, and the 4 openings are equally spaced on the inner wall of the micro-cell, so that the extraction of liquid, Replacement is more efficient. There are a total of 22 control channels,...

Embodiment 2

[0048] This example demonstrates that the microfluidic liquid operating system provided by the present invention can realize the replacement of liquid in the fish micropond by using the dye experiment.

[0049] Under the control of the vacuum pump drive and the solenoid valve, the liquid in the micro-pool is connected to the negative pressure through the micro-pipe and hose. Under the action of a negative atmospheric pressure, the liquid in the micro-pool for fish farming is completely sucked out of the chip within 10 seconds. , this process is a suction process. Under the drive of compressed air and the control of the solenoid valve, the liquid in the micro-pool is connected to positive pressure through micro-pipes and hoses. It is sent to the designated micro pool, and a certain height of liquid is accumulated in the micro pool, and this process is called the liquid delivery process. A pumping process and a liquid delivery process are collectively called a cycle. The liqui...

Embodiment 3

[0051] This example demonstrates that the microfluidic liquid operating system provided by the present invention can be used for drug treatment of fish embryos by using the automatic dyeing process of zebrafish embryos in the chip.

[0052] Normal zebrafish embryos were stained with green dye. First put the zebrafish embryos and the buffer solution into the fish micropool. The liquid aspiration process is carried out first, then the liquid feeding process is performed with the green dye, then the liquid absorption process is repeated, and finally the liquid feeding process is performed with the buffer solution. image 3 (a)-(c) show the process of normal zebrafish embryos being stained green after two cycles.

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Abstract

The invention relates to a microfluidic chip applicable to medicine screening of zebra fish embryos. The microfluidic chip is low in price, easy to prepare and suitable for large scale, high flux and automation. The microfluidic chip comprises at least one microfluidic layer, at least one control layer, a connecting layer and at least one micro cell, wherein the control layer is adjacent to the microfluidic layer; the connecting layer is used for connecting the adjacent microfluidic layer and the control layer; the micro cell is used for accommodating the zebra fish embryos and providing the growth environment for the zebra fish embryos; the microfluidic layer is provided with a microfluidic channel; the microfluidic channel is communicated with the internal space of the micro cell; the control layer is provided with at least one control channel; the microfluidic channel and the control channel are provided with at least one intersected point; and the connecting layer at the position of the at least one intersected point consists of an elastomer material and forms an elastomer valve. The communication state of the microfluidic channel can be controlled convenient through the elastomer valve, so that fully-automatic operation of medicine screening of the zebra fish embryos can be realized through an electromagnetic valve controlled by an external computer.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip which is cheap, easy to prepare, suitable for large-scale, high-throughput, and automatic drug screening for zebrafish embryos. Background technique [0002] Zebrafish is a model organism with transparent and highly permeable embryos, rapid development, relatively conservative regulatory processes, small size and easy operation. Using zebrafish for high-throughput drug screening has important applications in ecotoxicology, developmental genetics, and drug development. And many other fields have a wide range of applications. In the screening process, if the automatic liquid replacement step can be realized, the labor force and human error in the experiment can be greatly reduced. Although people have developed the technology of using high-density orifice plates and row guns to change liquid, this technology can only improve the efficiency of operation and cannot meet the...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/36C12M1/34C12Q1/02
Inventor 黄岩谊张博庞玉宏周宏伟刘新星周莹李文涛郑春红
Owner PEKING UNIV
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