Mesenchymal stem cell preservation liquid, and preparation method and application thereof

Abstract

The invention belongs to the field of biologic medicines and discloses mesenchymal stem cell preservation liquid, and a preparation method and application thereof. The mesenchymal stem cell preservation liquid contains AB cord blood plasma which is 5-15% of the volume of the preservation liquid and low molecular weight heparin calcium which is 70-80 AXaIU / ml. The AB cord blood plasma and mesenchymal stem cells come from the same provider. By means of the mesenchymal stem cell preservation liquid provided by the invention, the activity maintaining time of the mesenchymal stem cells can be prolonged (above 90% of activity is kept at 4-15 DEG C for 72 h); after the mesenchymal stem cells are preserved in the preservation liquid for 72h, the cells are normal in form, the proliferation and amplification capability of the cells cannot be influenced, and the phenotypic characteristics of the mesenchymal stem cells cannot be influenced; the mesenchymal stem cell preservation liquid disclosed by the invention is safe and reliable for clinical application; and, because the AB cord blood plasma and the cord mesenchymal stem cells come from the same placenta and belong to auto-plasma, the possibility of exogenous virus pollution is reduced.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a mesenchymal stem cell preservation solution and a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of stem cells derived from mesoderm with multi-lineage differentiation ability, which widely exist in various tissues of the body, can be cultured and expanded in vitro, and can differentiate into Cardiomyocytes, nerve cells and fat cells, etc. [0003] With the in-depth research on the biological characteristics and functions of mesenchymal stem cells by scholars at home and abroad, mesenchymal stem cells can be isolated from various tissues. Mesenchymal stem cells can support hematopoiesis and regulate immunity, and have broad clinical application prospects in hematopoietic reconstruction, tissue repair, and immunotherapy. Mesenchymal stem cells do not express MHC class II molecules and co-stimulatory fact...

AI Technical Summary

Problems solved by technology

[0008] But one of the shortcomings of the existing technology is that TC199 in the mesenchymal stem cell preservation solution is a cell culture medium, which is usually used for cell and tissue culture, and is not a nationally approved reagent that can be clinically applied to the human body. Complicated, the quality of culture media from different manufacturers varies, and imported reagents are expensive. In recent years, with the continuous development of the field of biological reagents, TC199 has gradually withdrawn from the media market and has been replaced by other media, so its source is limited

[0009] In short, although the existing technology uses TC199 as an active ingredient in the mesenchymal stem cell preservation solution to maintain cell viability for 24 hours, it is not conducive to the clinical promotion and use of mesenchymal stem cells in terms of reagent source, clinical application legitimacy, and reagent cost. of

[0010] Another disadvantage of the prior art is that it maintains the viability of mesenchymal stem cells for a short period of time. Mesenchymal stem cells can maintain more than 90% of their viability in the cell preservation solution of the prior art for only 24 hours, and with time Prolonged, the cell viability decreases, which means that after 24 hours, the cell viability will drop below 90%. However, stem cell therapy and clinical trials have been carried out in most cities in China and many foreign countries. Using existing means of transportation, 24 hours The viability maintenance time of mesenchymal stem cells obviously limits the application range of mesenchymal stem cells

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