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Endometrium stem cell protecting liquid and preparation method thereof

An endometrial stem cell and protection solution technology is applied in the field of endometrial stem cell protection solution and its preparation, which can solve the problems of difficult quality control, reduced recovery rate, impact on use, etc. The effect of slowing down the rate of metabolism

Inactive Publication Date: 2019-04-05
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Endometrial stem cells have high proliferative activity and self-regeneration ability, and have important clinical medical value in cell therapy and regenerative medicine. At present, endometrial stem cells can be cultured and expanded in vitro for scientific research, drug screening test, clinical Applications, etc. In order to conveniently take the endometrial stem cells cultured and expanded in vitro, it is generally necessary to freeze and recover the cultured endometrial stem cells. At present, the cryopreservation solution used for general endometrial stem It is divided into two types: serum-containing and serum-free. Serum-containing frozen storage solutions are complex in composition, expensive, and difficult to control in quality. Therefore, the application of serum-free frozen storage solutions is increasing. Serum-free frozen storage solutions mainly contain 70% serum-free medium, 20% bovine serum albumin and 10% dimethyl sulfoxide, the recovery rate of endometrial stem cells stored in serum-free cryopreservation medium increases with time The amplitude is reduced, which seriously affects the subsequent use

Method used

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  • Endometrium stem cell protecting liquid and preparation method thereof
  • Endometrium stem cell protecting liquid and preparation method thereof
  • Endometrium stem cell protecting liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A protective solution for endometrial stem cells, which is prepared from the following ingredients in parts by weight:

[0035] 1 part of heparin calcium, 20 parts of low molecular weight dextran, 1 part of papaverine, 5 parts of trehalose, 800 parts of water and 4 parts of Chinese medicine extract, wherein the Chinese medicine extract consists of the following parts by weight The ingredients are prepared from:

[0036]

[0037] Wherein, the preparation method of the endometrial stem cell protection solution is to place heparin calcium, dextran, papaverine, trehalose, and traditional Chinese medicine extracts in water and mix them evenly to obtain the product.

Embodiment 2

[0039] A protective solution for endometrial stem cells, which is prepared from the following ingredients in parts by weight:

[0040] 3 parts of heparin calcium, 30 parts of low molecular weight dextran, 1.5 parts of papaverine, 7.5 parts of trehalose, 900 parts of water and 5 parts of Chinese medicine extract, wherein the Chinese medicine extract consists of the following parts by weight The ingredients are prepared from:

[0041]

[0042] Wherein, the extracting method of Chinese medicine extract comprises the following steps:

[0043] 1) Take the formula amount of gallbladder green leaves, wash and crush them, place them in warm water at 50°C for extraction under reduced pressure twice, each time for 1 hour, the solid-liquid ratio of gallbladder green leaves and warm water at 50°C is 1:10, The degree of vacuum extraction is 0.7Mpa, and each extract is combined to obtain the extract of Folium Folium;

[0044] 2) Take the formula amount of Rhizoma Cyperi, Vitex and Alli...

Embodiment 3

[0048] A protective solution for endometrial stem cells, which is prepared from the following ingredients in parts by weight:

[0049] 5 parts of heparin calcium, 40 parts of low molecular weight dextran, 2 parts of papaverine, 10 parts of trehalose, 1000 parts of water and 6 parts of Chinese medicine extract, wherein the Chinese medicine extract consists of the following parts by weight The ingredients are prepared from:

[0050]

[0051] Wherein, the extracting method of Chinese medicine extract comprises the following steps:

[0052] 1) Take the formula amount of gallbladder green leaves, wash and crush them, place them in warm water at 70°C for extraction under reduced pressure twice, each time for 2 hours, the solid-liquid ratio of gallbladder green leaves and warm water at 70°C is 1:12, The degree of vacuum extraction is 0.9Mpa, and each extract is combined to obtain the extract of Folium Folium;

[0053] 2) Take the formula amount of Rhizoma Cyperi, Vitex and Alliu...

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Abstract

The invention provides endometrium stem cell protecting liquid and a preparation method thereof. The protecting liquid comprises 1-5 parts of heparin calcium, 20-40 parts of low-molecular dextran, 1-2parts of papaverine, 5-10 parts of mycose, 800-1000 parts of water and 4-6 parts of a traditional Chinese medicine extract. The endometrium stem cell protecting liquid does not contain serum, is lowin price, after stored endometrium stem cells are cryopreserved and recovered, the recovery rate is high, and along with passing of the time, the reduction rate of the recovery rate of the endometriumstem cells is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an endometrial stem cell protection solution and a preparation method thereof. Background technique [0002] Endometrial stem cells have high proliferative activity and self-regeneration ability, and have important clinical medical value in cell therapy and regenerative medicine. At present, endometrial stem cells can be cultured and expanded in vitro for scientific research, drug screening test, clinical Applications, etc. In order to conveniently take the endometrial stem cells cultured and expanded in vitro, it is generally necessary to freeze and recover the cultured endometrial stem cells. At present, the cryopreservation solution used for general endometrial stem It is divided into two types: serum-containing and serum-free. Serum-containing frozen storage solutions are complex in composition, expensive, and difficult to control in quality. Therefore, the application of serum-f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 董明珠宋莉莉潘新刘世红卢家堃
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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