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Method for monitoring drug resistance frequency of peronospora parasitica

A technology for drug resistance and downy mildew, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of isolation and purification of obligate parasitic downy mildew, and achieve the effect of convenient control and drug resistance management

Inactive Publication Date: 2012-08-22
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention overcomes the disadvantage that the current conventional drug resistance monitoring method cannot be used for the isolation and purification of obligate parasitic downy mildew, and provides a method for directly measuring the drug resistance frequency of downy mildew on detached leaves

Method used

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  • Method for monitoring drug resistance frequency of peronospora parasitica
  • Method for monitoring drug resistance frequency of peronospora parasitica
  • Method for monitoring drug resistance frequency of peronospora parasitica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. Monitoring of changes in drug resistance frequency after mixed culture of cucumber downy mildew sensitive strains and resistant strains for multiple generations.

[0017] 1. Materials and methods

[0018] (1) Test agent: 96% flumorph (flumorph) technical.

[0019] (2) Plants for testing: Cultivate the susceptible cucumber variety Changchun Mici in a seedling pot with a height of ф15cm and a height of 12cm, and place it in a greenhouse for cultivation. After the plants grow to the 5-leaf stage, collect the leaves of the third leaf position for use Disc production.

[0020] (3) Tested strain: Cucumber downy mildew strain K-1 (can be replaced by other common strains) is a strain sensitive to flumorph collected from the field in the laboratory, and the minimum inhibitory concentration of flumorph to the strain is 0.70 μg / mL; RK-1 is a mutant resistant to flumorph that was induced from the parental strain K-1 by ultraviolet induction method, and the mutant was g...

Embodiment 2

[0033] Embodiment 2, frequency monitoring of the resistance of cucumber downy mildew to metalaxyl

[0034] 1. Materials and methods

[0035] (1) Test agent: 95% metalaxyl original drug;

[0036] (2) Plants for testing: Cultivate the susceptible cucumber variety Changchun Mici in a seedling pot with a height of ф15cm and a height of 12cm, and place it in a greenhouse for cultivation. After the plants grow to the 5-leaf stage, collect the leaves of the third leaf position for use Disc production.

[0037] (3) Determination method of resistance frequency

[0038](1) Field bacterial strain collection: the test gathers the cucumber downy mildew disease sample from the cucumber greenhouse in the suburb of Jinning County, Kunming City, Yunnan Province, gathers according to 5 points method sampling, every point gets 10 diseased leaves, then the diseased leaves collected by 5 points Put it in a fresh-keeping bag and bring it back indoors.

[0039] (II) Preparation of mixed spore su...

Embodiment 3

[0048] Embodiment three, grape downy mildew bacteria resistance monitoring to flumorph and metalaxyl

[0049] 1. Materials and methods

[0050] (1) Drugs for testing: 95% metalaxyl and 96% flumorph.

[0051] (2) Tested plant: the tested plant is a downy mildew susceptible variety Red Globe (Vitis vinifera cv. Red Globe).

[0052] (3) Determination method of resistance frequency:

[0053] (1) field bacterial strain collection: test collects downy mildew disease sample from Shilin County, Kunming City, Yunnan Province and Fumin County Suburb Vineyard, Wenshan Prefecture Yanshan County Vineyard, gathers samples according to 5-point method, and every point gets 10 diseased leaves , and then put the diseased leaves collected at the 5 points into fresh-keeping bags and bring them back indoors.

[0054] (II) Preparation of mixed spore suspension: In the room, the impurities on the diseased leaves collected in the field and the mature sporangia were washed with sterile water, and c...

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Abstract

The invention discloses a method for monitoring the drug resistance frequency of peronospora parasitica of a plant. The method comprises the following steps of: A1, collecting a peronospora tabacina sample in an area to be monitored and carrying the peronospora tabacina sample back to a room; and cultivating and inducing under a dark condition to generate new sporangiums at a temperature of 19 DEG C; A2, eluting and mixing the all newly borne sporangiums collected on a diseased leaf to obtain a sporangium suspension; A3, respectively vaccinating the sporangium suspension with a back face of a leaf disc, which is manufactured by a health separated plant leaf by using a sessile drop method, wherein the leaf disc is soaked with sterile water for 30 minutes and is soaked with two liquid medicines (with a minimum inhibition concentration of a bactericide to be detected) for 30 min in advance; A4, arranging the vaccinated leaf disc in a culture dish for moisturizing absorbent paper so as to culture in a moisturized manner; and A5, measuring and calculating a frequency of a drug resistance strain. The method is simple and easy to learn and is easy to operate, and has low requirements on an experiment instrument. Furthermore, the method can be used for more accurately reflecting the drug resistance condition of a field drug resistance group and has higher actual application values.

Description

technical field [0001] The invention relates, in particular, to a method for frequency monitoring of downy mildew drug resistance. Background technique [0002] Downy mildew is an important pathogen of plants, and the disease caused by it is commonly called downy mildew. Downy mildews are highly destructive and harmful to host plants, and most plant diseases caused by downy mildews are difficult to control. In particular, fruits, vegetables and economic crops are susceptible to downy mildew infection, which has a short incubation period and frequent re-infection. The pathogen can develop rapidly within a growing season of the plant, causing disease epidemics and causing serious losses in agricultural production. [0003] At present, chemical pesticide control is still the main control method, and it plays an important role in the control of downy mildew. Before the mid-1970s, the agents used for the control of downy mildew were mainly some protective fungicides, such as ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
Inventor 朱书生杨敏梅馨月邓维萍杜飞何霞红王海宁李成云朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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