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Bacillus amyloliquefaciens GN03 and application thereof

A technology for dissolving starch spores and GN03, applied in the field of microorganisms, can solve problems such as poor ecological adaptability and inability to achieve crop growth promotion effect, and achieve the effect of fast growth rate and good growth promotion effect.

Active Publication Date: 2017-08-29
重庆千明农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the relevant researches are based on sterilizing the soil or matrix, and then carrying out research on the effects of inoculating PGPR on plant growth and other effects. Poor sex and other problems, unable to compete with indigenous microorganisms and "annihilate", unable to achieve the growth-promoting effect on crops

Method used

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  • Bacillus amyloliquefaciens GN03 and application thereof
  • Bacillus amyloliquefaciens GN03 and application thereof
  • Bacillus amyloliquefaciens GN03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation and identification of embodiment 1GN03 bacterial strain

[0027] (1) Test soil

[0028] The purple soil in Beibei District, Chongqing City was collected, and the sampling site was a planting area where vegetables had been grown for more than ten years, and the yield and quality of vegetables were high. After mixing the soil samples evenly, put them in an ice bucket and take them back to the refrigerator at 4°C for storage. The basic physical and chemical properties of the tested soils are shown in Table 1.

[0029] Table 1 Basic physical and chemical properties of the tested soil

[0030]

[0031] (2) culture medium

[0032] Nitrogen-fixing bacteria selection medium (also known as: Azobia nitrogen-free medium): 0.2g CaSO 3 ;0.2g KH 2 PO 4 ; 0.2gNaCl; 5.0gCaCO 3 ;0.2g MgSO 4 ·7H 2 O; glucose 5.0g; agar 15g; deionized water 1000ml; pH natural.

[0033] LB medium: 10g NaCl; 5g yeast powder; 10g tryptone; 15g agar; 1000mL deionized water; natural p...

Embodiment 2

[0037] The mensuration of embodiment 2GN03 bacterial strain nitrogen fixation ability

[0038] To prepare the nitrogen-free liquid culture medium of Ashubei, take 100mL of the medium and divide it into 250mL Erlenmeyer flasks, after autoclaving, inoculate the isolated and purified GN03 strain, and set up a blank control without inoculation, culture at 28°C and 120r / min 7d, dilute the cell concentration of the fermentation broth to 1×10 8 cfu / mL, spare;

[0039]Then prepare the nitrogen-free liquid culture medium of Ashubei, take 100mL culture medium and put it into 250mL Erlenmeyer flask, after sterilizing at 121℃ for 30min, the test group was inoculated with 1×10 8 cfu / mL GN03 strain fermentation broth 2mL, the control group was added 2mL non-inoculated Axubei nitrogen-free liquid medium, the parallel experiment was repeated three times, and cultured at 28°C and 120r / min for 7 days. Use the diluted plate colony counting method (azotobacter selective medium, cultivated at 28...

Embodiment 3

[0043] The identification of embodiment 3GN03 bacterial strain

[0044] 1) Morphological identification

[0045] After the preserved strain GN03 was activated by the selection medium, the morphological characteristics of the colonies were observed; then the strains were picked for Gram staining, capsule staining and spore staining, and then the bacterial morphology was observed under a microscope.

[0046] Spore staining of GN03 strain as figure 1 As shown, the colony morphology of GN03 strain on Ashubei nitrogen-free medium is white and translucent, smooth and moist, round and slightly raised, with neat edges. GN03 strain Gram staining results are as follows figure 2 As shown, the Gram-stained bacteria are positive, the spores are oval, the sporangia are not inflated, the growth position is mesophytic, and the capsule is hypertrophic. Cultivate for 1-2d, the colony diameter is 2-3mm, and cultivate for 3-5d, the colony diameter is 6-8mm.

[0047] 2) Physiological and bioc...

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Abstract

The invention relates to a bacillus amyloliquefaciens GN03 strain and application thereof. Bacillus amyloliquefaciens GN03 is separated from purple soil with high yield and quality of vegetables, a bacterial colony is white and semitransparent, is smooth and moist, and has circular tiny projections and neat edges, gram stain thallus is positive, spores are oval, sporangia are not expanded and grown in the middle, and capsules are plump. After the bacillus amyloliquefaciens GN03 cultures for 1-2 days, the diameter of the bacterial colony is 2-3 mm; after the bacillus amyloliquefaciens GN03 cultures for 3-5 days, the diameter of the bacterial colony is 6-8 mm. On an ashby nitrogen-free medium, when the concentration of the thallus is 1.5* 106 cfu / ml, after the bacillus amyloliquefaciens GN03 cultures for 7 days at the temperature of 28 DEG C and at the speed of 120 r / min, the nitrogen fixation quantity of fermentation liquor reaches up to 91.5+ / - 2.69 mg / L, and the free living nitrogen fixation ability is high.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to Bacillus amyloliquefaciens GN03 and an application thereof. Background technique [0002] In 1904, German microbiologists first proposed the concept of plant rhizosphere (rhizosphere), which refers to the area of ​​several millimeters around plant roots. The plant rhizosphere is a microenvironment at the interface between the plant root system and the soil, and it is the interaction site where the soil, root system and microorganisms are closely combined. The rhizosphere microbial population is mainly composed of bacteria. According to their different effects on plants, rhizobacteria can be roughly divided into beneficial bacteria (2%-5%), harmful bacteria (8%-15%) and Neutral bacteria (80%-90%) three categories. Plant growth-promoting rhizobacteria (PGPR) refers to a group of bacteria that can live freely in the soil or colonize in the rhizosphere of plants, which can promote plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/205C12R2001/07
Inventor 杨星勇陈姗姗张宇亭申鸿李艳梅
Owner 重庆千明农业发展有限公司
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