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Fluid medium made from celery and suitable for phytophthotacactorum to generate sporangium

A liquid culture medium, the technology of Phytophthora malaria, applied in the method of using spores, the method based on microorganisms, the microorganisms and other directions, can solve the problems of complicated operation and various sporangia raw materials, etc., and achieves a simple and easy-to-learn procedure and high practical application value. Effect

Inactive Publication Date: 2012-12-26
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention overcomes the disadvantages of producing sporangia by inducing the production of sporangia with various raw materials and complicated operation in the current hydroponic method of Phytophthora viridans hyphae, and provides a simple and easy method for culturing and producing sporangia in a liquid medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0008] Embodiment: Comparison of sporangia production ability and zoospore release ability difference between celery liquid culture medium and other liquid culture medium

[0009] 1. Production of liquid medium:

[0010] (1) Cucumber culture medium (10%): Wash and chop the cucumber and squeeze the juice in a juicer. The resulting juice is filtered with four layers of gauze and mixed with 0.02% CaCO 3 Deionized water was mixed at a volume ratio of 1:9.

[0011] (2) Cucumber culture medium (50%): Wash and chop the cucumber and squeeze the juice in a juicer. The resulting juice is filtered with four layers of gauze and mixed with 0.02% CaCO 3 Deionized water was mixed at a volume ratio of 1:1.

[0012] (3) Tomato culture medium (10%): Wash and chop the tomatoes and squeeze the juice in a juicer. The resulting juice is filtered with four layers of gauze and mixed with 0.02% CaCO 3 Deionized water was mixed at a volume ratio of 1:9.

[0013] (4) Tomato medium (50%): Wash and ch...

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PUM

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Abstract

The invention discloses a fluid medium made from celery and suitable for phytophthotacactorum to generate sporangium. A preparation method of the fluid medium particularly comprises the following steps: removing leaves, washing and cutting up celery, adding deionized water to celery in a w / v ratio being 1:1, and juicing by using a juicer; filtering the obtained celery juice by using four layers of gauze, diluting with deionized water containing 0.02% (w / v) CaCO3 till the celery juice is 10%-50%(v / v); and subpackaging and autoclaving for 20min at 121 DEG C under 0.103MPa; pouring autoclaved fluid medium into culture dishes with diameter being 9cm, wherein 15ml of autoclaved fluid medium into each culture dish. The raw material of the fluid medium are low in cost and can be acquired easily, the steps for making the fluid medium and generating sporangium are simple and easy to learn, and the sporangium generation efficiency of phytophthotacactorum is improved greatly, so great convenience is provided for preparation of pathogenic bacteria inoculants.

Description

technical field [0001] The invention relates to a liquid culture medium for producing sporangia of plant pathogen Phytophthora cactorum in the field of plant protection. Background technique [0002] Phytophthora cactorum Schroet, belonging to the genus Phytophthora oomycetes, is an important plant pathogen widely distributed in the world, and can infect about 200 species of plants in about 60 families and 50 genera. Phytophthora usually overwinters in diseased debris and soil as mycelium and oospores. When the conditions are suitable in the following year, the mycelium directly infects the host, or forms a large number of zoosporangia and spreads to the aboveground part to infect the stems and leaves. Sporangia play a very important role in the transmission and spread of the disease. Generally, it is necessary to prepare sporangia suspensions for pathogenicity determination or other related biological characteristics research in the laboratory. At present, the method for...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
Inventor 杨敏朱书生朱贵李梅馨月何霞红李成云朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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