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Method for indoor culture breeding of Plasmopara viticola

A grape downy mildew and downy mildew technology, applied in the biological field, can solve the problem of limiting the resistance of grape downy mildew, monitoring drug resistance, screening new agents, risk assessment of drug resistance, pathogenicity differentiation, inability to reproduce in large numbers, and inability to isolate and cultivate, etc. problems, to achieve high practical application value, overcome difficulties in survival, and achieve the effect of simple and easy-to-learn methods

Inactive Publication Date: 2013-06-26
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Grape downy mildew is an obligate parasite, which cannot be isolated and cultured on the medium and cannot reproduce in large numbers indoors, which severely limits the indoor monitoring of drug resistance, screening of new agents, risk assessment of new drug resistance and The development of research work such as pathogenic differentiation
Researchers have to continuously go to the field to collect the bacteria for the test in a short period of time, which not only increases the workload, but also greatly increases the test cost

Method used

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  • Method for indoor culture breeding of Plasmopara viticola
  • Method for indoor culture breeding of Plasmopara viticola
  • Method for indoor culture breeding of Plasmopara viticola

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The condition of embodiment 1 zoospore release

[0048] 1. Materials and methods

[0049] (1) Collect grape downy mildew fungus samples: Take the grape downy mildew fungus sample in Baoding, Hebei as a representative, use the 5-point method for sampling, and take 10 diseased leaves of grape downy mildew at each point, and use an incubator at a low temperature of 0-10°C Save and bring back indoors;

[0050] (2) Preparation of sporangia suspension: Use a QWJ-150 air compressor with a pressure of 0.08 MPa to drive a throat sprayer to wash away the impurities on the surface of the diseased leaves and the mature sporangia on the surface of the downy mildew with sterile water. Place in a petri dish with wet filter paper, seal the petri dish with plastic wrap, and incubate in the dark at 18°C ​​for 24 hours to induce new sporangia; use a QWJ-150 air compressor with a pressure of 0.08 MPa to drive the throat sprayer Wash the fresh sporangia with sterile water, filter with Wha...

Embodiment 2

[0058] The selection of embodiment 2 grape varieties and blades

[0059] The samples of grape downy mildew in Baoding, Hebei are the representative, and the grape variety is the European and American hybrid Gaozi. Collect the first to sixth fully expanded healthy leaves on the top of each branch of the grape plant, and the leaf age of the leaves is consistent and the grape plants and the leaves have not been used in the growth period and no downy mildew occurs; Bacteria water was washed 3 times, and the prepared sporangia content was 1×10 5 -1.5×10 5 pcs / mL suspension (the method is the same as in Example 1), placed in a 4°C refrigerator for 20 minutes at room temperature for a period of time, and when the temperature of the suspension was at room temperature, it was used to inoculate the sporangia suspension of P. viticilis. When inoculating, use a QWJ-150 air compressor with a pressure of 0.08 MPa to drive the throat sprayer to spray the sporangia suspension evenly on the ...

Embodiment 3

[0064] The selection of embodiment 3 inoculum concentration

[0065] The samples of grape downy mildew in Baoding, Hebei are the representative, and the grape variety is the European and American hybrid Gaozi. Collect the first 1-3 fully expanded healthy leaves at the top of the branches of the grape plants, the leaf age of the leaves is the same and the grape plants and leaves have not been used in the growth period and no downy mildew occurs; the content of the prepared sporangia is 1 × 10 3 , 1×10 4 , 1×10 5 , 1.5×10 5 Each / mL suspension (method is the same as in Example 1), placed at room temperature for a period of time after 20 min low-temperature treatment in a refrigerator at 4°C, and used to inoculate grape downy mildew sporangia suspension when the temperature of the suspension is at room temperature (method is the same as in Example 1) 2). Place glass rods on two layers of sterile water-soaked filter paper in a petri dish, then place the inoculated leaves face d...

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Abstract

The invention provides a method for the indoor culture breeding of Plasmopara viticola. The method comprises the following steps: 1, acquiring a Plasmopara viticola sample; 2, preparing a sporangium suspension; 3, selecting the grape variety and blade; 4, inoculating the sporangium suspension; and 5, carrying out blade moisturizing culture. The method has the advantages of simplicity, easy learning, low requirements on a tester, and culturing for 7-10 days under a same environment condition after blade morbidity, overcomes the defects comprising difficult survival, no indoor mass propagation of the Plasmopara viticola sample, and sporangium suspension insufficiency after the previous inoculation of the Plasmopara viticola, can be used for monitoring the Plasmopara viticola and determining the pathogenicity, and has very high practical application values.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for indoor cultivation and propagation of grape downy mildew. Background technique [0002] At present, grape downy mildew (Grape downy mildew) is the most important disease in grape planting. It is distributed in all grape producing areas in my country. It mostly occurs in areas and years with more rainfall. September is the peak season. It mainly harms leaves, and can also infect young tissues such as young shoots, inflorescences, and young fruits. In the epidemic year of the disease, the diseased leaves are scorched and fall early, the diseased shoots are twisted and stunted, which seriously affects the tree vigor and yield. As of 2010, the national viticulture area reached 552,000hm 2 , the incidence rate in severe disease areas is above 70%. [0003] Grape peronospora is an obligate parasitic fungus, and the diseased part of the leaf produces a white frosty mold laye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 马志强毕秋艳张小风王文桥韩秀英
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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