76 results about "Prokaryote organisms" patented technology

This invention provides a process for control in oil and gas wells and related facilities of prokaryote caused souring, fouling and corrosion by reduction of problematic prokaryotes with naturally occurring lysing organisms, particularly sulfate-reducing prokaryotes by proliferating suitable virulent lysing organisms under conditions in which problematic prokaryotes thrive, including in a gas production wellbore. The process provides in situ proliferation of virulent lysing organism in a wellbore by providing both virulent lysing organisms and their host prokaryotes to selectively grow an effective control amount and concentrations of lysing organisms in a well formation.

The invention discloses a recombinant DNA (deoxyribonucleic acid) fragment containing a roundup ready gene and application thereof. The recombinant DNA fragment provided by the invention comprises the following components from 1) to 3): 1) a promoter; 2) the roundup ready gene which is promoted by the promoter to be transcribed, the nucleotide sequence of the roundup ready gene is a) or b), wherein a) represents a sequence 2 in a sequence table, and b) represents a protein which has at least 98% of identity with the sequence 2 in the sequence table and is shown by a coding sequence 9; and 3) tanscription termination sequence. Compared with the transgenic corn which contains the recombinant DNA fragment in which prokaryote roundup ready gene G2-aroA is transplanted, the transgenic corn which is transplanted with the recombinant DNA fragment provided by the invention is obviously higher in the expression quantity of G2-aroA protein; and the transgenic corn is also obviously higher in the glyphosate tolerance.

An improved method for rapid identification of microorganisms is disclosed, along with sequences of PCR primers optimized for this purpose. The primers are designed based on information analysis of sequences from a large number of organism to amplify certain segments of genomic DNA whose sequences are unique among different organisms. The PCR products are compared with a DNA sequence database to obtain the identity of the microorganisms. This approach provides an accurate and fast identification and taxonomic assignment of microbial species.

Provided herein are phenylalanine ammonia-lyase (PAL) variants produced by prokaryotes, wherein such prokaryotic PAL variant has a greater phenylalanine-converting activity and / or a reduced immunogenicity as compared to a wild-type PAL. Further provided are compositions of prokaryotic PAL and biologically active fragments, mutants, variants or analogs thereof, as well as methods for the production, purification, formulation, and use of such compositions for industrial and therapeutic purposes, [e.g., treating hyperphenylalaninemia, (including phenylketonuria)], and other disorders (including cancer).

The invention is directed to bioconjugate vaccines comprising N-glycosylated proteins. Further, the present invention is directed to a recombinant prokaryotic biosynthetic system comprising nucleic acids encoding an epimerase that synthesizes an oligo- or polysaccharide having N-acetylgalactosamine at the reducing terminus. The invention is further directed to N-glycosylated proteins containing an oligo- or polysaccharide having N-acetylgalactosamine at the reducing terminus and an expression system and methods for producing such N-glycosylated proteins.

This invention generally relates to a composition for developing novel anti-cancer drugs and / or vaccines and producing microRNA precursor (pre-miRNA) and / or its shRNA homologs / mimics / derivatives, and a method thereof. The present invention also relates to a use of a composition in producing novel prokaryote-produced microRNA precursor (pro-miRNA) capable of being delivered into human cells and processed by the cells into microRNA-like effectors to elicit specific silencing effects on certain targeted oncogenes, subsequently leading to a therapeutic result of tumor suppression and cancer therapy. Specifically, the method of the present invention includes inducing an expression of the pre-miRNA / pro-miRNAs, particularly human pre-miR-302, in prokaryotes through pol-2 or pol-2-like RNA promoter. Most importantly, the composition of the present invention is further a novel pre-miRNA-based drug that is capable of reprogramming the malignant properties of high-grade human liver cancers into a low-grade benign or even relatively normal stage—a mechanism called “Cancer Reversion”.

The invention belongs to biology technical field, concretely relates to a method for realizing a therapeutic gene on anaerobic tissue targeting delivery and selectivity stabilization expression. According to the invention, a prokaryotic bacteria nitroso reductase promoter PnirB induced by prokaryotes anaerobe is taken as composition of a therapeutic gene promoter, anaerobic targeting bacteria, low copy plasmid and the like, so that therapeutic gene enables an specific expression under low oxygen or hypoxia environment in vivo and in vitro. The method for realizing the therapeutic gene on the anaerobic tissue targeting delivery and selectivity stabilization expression is effectively treating the anaerobic tissue diseases containing tumor, or treating the anaerobic tissue diseases comprising tumor by combining other chemical medicaments and traditional Chinese medicines, and can be used for preparing antitumor drugs.

Methods are presented for binning metagenomic sequences that leverage long reads from a single-molecule long-read sequencing technology and utilize DNA methvlation signatures inferred from these readsto resolve individual reads and assembled contigs into species- and strain-level clusters. Methods for deconvolving prokaryotic organisms in a microbiome sample are presented. Methods for mapping mobile genetic elements to their host organisms in a microbiome sample are also presented.

The invention described herein generally relates to glycoengineering host cells for the production of glycoproteins for therapeutic use. Host cells are modified to express biosynthetic glycosylation pathways. Novel prokaryotic host cells are engineered to produce N-linked glycoproteins wherein the glycoproteins comprise polysialic acid or blood group antigens.

A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.

The disclosure provides methods to assemble genomes of eukaryotic or prokaryotic organisms. The disclosure provides methods for haplotype phasing and meta-genomics assemblies. The disclosure providesa streamlined method for accomplishing these tasks, such that intermediates need not be labeled by an affinity label to facilitate binding to a solid surface. The disclosure also provides methods andcompositions for the de novo generation of scaffold information, linkage information, and genome information for unknown organisms in heterogeneous metagenomic samples or samples obtained from multiple individuals. Practice of the methods can allow de novo sequencing of entire genomes of uncultured or unidentified organisms in heterogeneous samples, or the determination of linkage information fornucleic acid molecules in samples comprising nucleic acids obtained from multiple individuals.

The invention provides a PCR (polymerase chain reaction) kit with internal amplification control and for detecting shigellae in food, relating to the technical field of biology. A method is convenient to use, has the advantages of good reliability, short detection period, high sensitivity, strong specificity, low cost and simple operating steps, is suitable for high throughput operation and standardized operation, can avoid misjudgment caused by false negative results obtained because DNA (deoxyribonucleic acid) polymerase inhibitors exist in samples, and can be used for detecting shigellae in various food. The method comprises the following steps of: (1) utilizing a pair of specific primers ipaHF / ipaHR which are designed by primer premier 6.0 software and aim at shigellae, then adding primers 27F / 1492R amplifying a prokaryote 16S rDNA conserved sequence as the internal amplification control and carrying out amplification by the two pairs of primers jointly; (2) carrying out enrichment culture on a sample to be detected for 6-8 hours and extracting genomic DNA from enrichment fluid obtained after culture as a template by adopting a boiling and freeze-thaw method to carry out amplification; and (3) preparing 25mu l of reaction system with PCR reaction premix of the kit to carry out amplification and observing the detection results under an ultraviolet lamp after agarose gel electrophoresis.

The invention provides a construction method of an inducible endogenous CRISPR-Cas system and application of the inducible endogenous CRISPR-Cas system in simultaneous editing of multiple genes, and belongs to the technical field of genomics, genetic engineering and biology. According to the inducible endogenous CRISPR-Cas system disclosed by the invention, only one plasmid containing a CRISPR cluster containing a plurality of spacers and a plurality of donor DNA fragments and a strain with the inducible endogenous CRISPR-Cas system need to be constructed. A large number of CRISPR-Cas effect compounds generated during induction are utilized to complete simultaneous targeted cutting of a plurality of genes and homologous recombination repair of DNA of a plurality of donors, so that simultaneous editing of the plurality of genes is realized. According to the method, the gene editing efficiency is high, the multi-gene editing of the prokaryote can be completed at one time or by fewer times, the required time, energy and cost are greatly saved, and the applicable host range is wide, so that the method has good practical application value.

The present invention is directed to a cell-free system for producing a glycosylated protein. This system comprises an isolated oligosaccharyltransferase capable of transferring a glycan from a lipid carrier molecule to a glycoprotein target, one or more isolated glycans, where each glycan is linked to a lipid carrier molecule, and a glycoprotein target comprising one or more glycan acceptor amino acid residues or a nucleic acid molecule encoding said glycoprotein target. The present invention further relates to kits and methods for producing a glycosylated protein in this cell-free system.

The invention relates to a plasmid containing angiotensin converting enzyme 2 (ACE2), a prokaryotic cell containing the plasmid and a method for realizing fermentative expression of ACE2 by using the prokaryotic cell as a host cell. The invention also relates to a method for producing ACE2 by using a prokaryote. The method comprises the following steps: constructing the plasmid containing a natural ACE2 sequence, knocking out the ACE2 sequence of a transmembrane region and an intracellular region, or further knocking out the ACE2 sequence of a part of extracellular region to serve as an exogenous gene; introducing the plasmid into a host prokaryote; culturing the host prokaryote, and expressing ACE2 in a culture; and extracting and purifying ACE2 from the culture.