The invention belongs to the field of cells and in particular relates to an efficient
induced pluripotent stem cell reprogramming method for blood cells. The method comprises the following steps: S1,extracting
monocyte from a
blood specimen, and performing selective culture with an amplification culture medium so as to obtain erythrocyte
progenitor cells; S2, introducing a
free carrier with at least one potentiality
determinant factor into the obtained erythrocyte
progenitor cells; S3, culturing the erythrocyte
progenitor cells with the
free carrier by using a pluripotent
stem cell inductionculture medium, and performing induction in a feeding layer
free system so as to obtain reprogrammed
intermediate state cells; S4, after induction is completed, replacing the pluripotent
stem cell induction culture medium in the step S3 by a pluripotent
stem cell culture medium to maintain culture, thereby obtaining cells that potentiality
determinant factor expression is vanished and expression of endogenous pluripotent genes POU5F1, NANOG, TRA-1-60 and TRA-1-81 is activated, namely the induced pluripotent stem cells. The method has the beneficial effects that pluripotent stem cells without endogenous
gene components can be efficiently induced, and the method is applicable to preclinical study and clinical application.