Broad bean rhizobium with drought tolerance and application thereof

Abstract

The invention discloses broad bean rhizobium with drought tolerance and application of the broad bean rhizobium. The separated broad bean rhizobium strain with drought tolerance is obtained from a broad bean host, the strain is subjected to shape, physical and chemical property and whole genome analysis and identification, the strain belongs to a new strain in the bacteria field, proteobacteria, alpha-proteobacteria, rhizobium, rhizobiaceae and rhizobium, and the microbial preservation number of the strain is CGMCC No.24092. According to the broad bean rhizobium provided by the invention, PEG (Polyethylene Glycol) is used for simulating drought stress, a broad bean artificial water control potted plant drought test and a rain-fed agricultural area field inoculation verification test prove that the broad bean rhizobium provided by the invention has the characteristics of drought tolerance, efficient nodulation, strong nitrogen fixation capability and the like; and the broad bean biomass and yield are increased, and the broad bean has application prospects in the aspects of improving the stress resistance of leguminous crops, promoting growth, improving the yield and the like.

Description

technical field [0001] The invention relates to Rhizobium, in particular to Rhizobium broad bean isolated from a broad bean host with drought resistance and its application in improving the drought resistance of leguminous crops, promoting growth or improving the yield thereof, belonging to Rhizobium broad bean Separation and its fields of application. Background technique [0002] Rhizobium is a kind of α- and β-proteobacteria that can fix free nitrogen in the atmosphere and establish a symbiotic relationship with leguminous plants. Nitrogen source, can fix and provide about 200kg N / ha in one growing season, much higher than soybean. However, there is a strong selectivity between rhizobia and legumes. Only by inoculating rhizobia that match legumes can an effective symbiotic nitrogen fixation system be formed, and the biological nitrogen fixation can be fully exerted. Although the number of indigenous rhizobia is relatively small due to drought and lack of water, the nodu...

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Problems solved by technology

[0004] At present, soybeans are the most popular inoculation of high-efficiency rhizobia on leguminous crops. However, there are few reports on the promotion and application of high-efficiency rhizobia in broad grains and leguminous crops with relatively small planting areas. Most of them focus on the biogeographical distribution of broad bean rhizobia In terms of genetic diversity and phylogenetic development, the research on the stress resistance of faba bean rhizobia and the improvement of faba bean stress resistance by grafting have not been carried out in depth, and there has been no substantial work progress, especially for the stress resistance of faba bean rhizobia in the arid areas of Northwest China. Fewer sex studies reported

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