52 results about "Aeromonas salmonicida" patented technology

The invention relates to Bacillus amyloliquefaciens V4. The Bacillus amyloliquefaciens V4 is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC NO. 10149. The Bacillus amyloliquefaciens V4 strain and / or microbial agent can produce bacteriocin for antagonizing vibrio pathogens and pathogenic aeromonas at seedling growing and culture temperatures of common culture water, inhibit vibrio vulnificus, vibrio natriegen, vibrio harveyi, black sea vibrio, aeromonas hydrophila and aeromonas salmonicida growth and breeding, effectively control aquatic animal infectious disease generation and propagating caused by vibrio pathogens and pathogenic aeromonas, reduce aquatic animal mortality and a forage coefficient and promote aquatic animal weight gain.

The invention relates to an aeromonas salmonicida phage, a bactericidal composition comprising the same and an application of the bactericidal composition. The phage is capable of quickly inhibiting growth of Atlantic aeromonas salmonicida within a short period of time and inhibiting generation of resistance of the pathogenic bacterium phage. In addition, the bactericidal composition comprising the phage is safe and effective, and is high in specificity and low in production cost, and the disadvantages of single phagotherapy are compensated in the control process of pathogenic bacterium.

The invention belongs to the technical field of fish biological products and cultured fish disease prevention and control, and in particular relates to an aeromonas salmonicida inactivated vaccine and applications of the aeromonas salmonicida inactivated vaccine. The antigen of the inactivated vaccine is aeromonas salmonicida subsp.masoucida, and the bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 19, 2013, and is assigned with the accession number of CGMCC No.7335. The inactivated vaccine can effectively induce aquaculture fish to generate specific immune response, and provide effective protection, and a material for preparing the aeromonas salmonicida inactivated vaccine is low in cost and is safe to the environment and the host.

The invention relates to aeromonas salmonicida bacteriophage, a sterilization composition with the same and application of the same. The bacteriophage is capable of rapidly inhibiting growth of Atlantic aeromonas salmonicida within a short time, and inhibiting generation of resistance of the pathogenic bacterium bacteriophage. In addition, the sterilization composition with the bacteriophage is safe and effective, high in specificity and low in production cost, and defects of single bacteriophage phagotherapy can be made up in the control process of pathogenic bacteria.

The invention relates to aeromonas salmonicida bacteriophage, a sterilization composition with the same and application of the same. The bacteriophage is capable of rapidly inhibiting growth of Atlantic aeromonas salmonicida within a short time, and inhibiting generation of resistance of the pathogenic bacterium bacteriophage. In addition, the sterilization composition with the bacteriophage is safe and effective, high in specificity and low in production cost, and defects of single bacteriophage phagotherapy can be made up in the control process of pathogenic bacteria.

The invention relates to an aeromonas salmonicida phage, a bactericidal composition containing the aeromonas salmonicida phage and application of the aeromonas salmonicida phage and the bactericidal composition. The phage can rapidly inhibit the growth of Atlantic aeromonas salmonicida in a short time and inhibit the generation of resistance to the pathogenic bacterium phage. In addition, the bactericidal composition of the phage is safe and effective and is high in specificity, the production cost is low, and the defects of a single phage therapy in a pathogen control process are made up.

The invention relates to a rapid detection primer for aeromonas salmonicida subsp.salmonicida and an application of the rapid detection primer and belongs to the technical field of biological detection. According to the rapid detection primer, the fstC gene is taken as a target gene; three pairs of DNA primers are designed and put into an amplification buffer solution with a DNA template to be detected for amplification in a constant-temperature environment of 63 DEG C, and then the reaction is terminated in a constant-temperature environment of 80 DEG C and the amplification product is detected. The rapid detection primer for the aeromonas salmonicida subsp.salmonicida, namely the LAMP detection primer, has the advantages of good specificity, high sensitivity, short consumed time, and simple and convenient operations. The primer can be applied to the detection of the aeromonas salmonicida subsp.salmonicida, and has a certain application prospect in the aspects of identification and monitoring of pathogen species, disease quarantine and the like of aquatic animals.

The invention relates to Aeromonas salmonicida phage, a bactericidal composition containing the same and application of the same. The Aeromonas salmonicida phage may inhibit the growth of Atlantic Aeromonas salmonicida quickly within a short time, and inhibit the occurrence of resistance in the phage. In addition, the bactericidal composition containing the Aeromonas salmonicida phage is good in safety and effect, high in specificity and low in production cost, and the defects of single phage therapy during control of pathogenic bacteria are made up.

The invention discloses Tai Lake reed marsh bottom sediment MC (microcystic) toxin degrading bacterium and an MC-LR degrading method, and belongs to the technical field of environment-friendly water biological treatment. The collection number of microcystic toxin degrading bacterium JZ-4 is CGMCC No.14051, and the suggested classification name is Aeromonas salmonicida subsp.salmonicida. One strain of indigenous bacterium JZ-4 with microcystic toxin degrading ability is screened from the bottom sediment at the root of reed marsh collected from Baidu Port of Tai Lake, the MC-LR degrading rate reaches 88.86%, and the broad prospect is realized for removal of MCs from the drinking water source. A bacterium screening source is provided for degrading of MCs by microorganisms.

An Aeromonas salmonicida subspecies (Aeromonas almonicidasubsp. masoucida) is isolated from Cynoglossus semilaevis Gunther cultured in Shandong Province, China, the preservation number of the Aeromonas almonicidasubsp. masoucida is CCTCCM2018085. A pathogenic bacteria strain of ascites disease is isolated from the Cynoglossus semilaevis Gunther for the first time. Antigens of the strain contain protective antigens against Aeromonas salmonicida, the strain is suitable for preparing various vaccines against the Aeromonas salmonicida, and is capable of reducinge losses caused by diseases in aquaculture, reducing or eliminating abuse of chemical drugs such as disinfectants and antibiotics caused by the Aeromonas salmonicida, and significantly improving the safety of aquaculture animals and products, and reducing bio-safety risks caused by increase of drug resistance of pathogenic bacteria.

Disclosed is a newly identified and characterized type III secretion system in Aeromonas salmonicida. The invention also encompasses the use of components of the novel secretion system in immunoprotection against A. salmonicida infection, as well as other diagnostic and therapeutic uses thereof.

A manufacturing method of separating and purifying neoagarooligosaccharides having degrees of polymerization of 2-22 includes the steps of adding crude enzyme solutions with agarases produced by Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108, respectively, into an agar polysaccharide extract solution, and obtaining a neoagarooligosaccharides solution after a hydrolysis on the algal polysaccharide (such as agar) is performed; performing a separation to the neoagarooligosaccharides solution by a ultrafiltration (UF) system to obtain a neoagarooligosaccharide eluent; performing a separation to the neoagarooligosaccharide eluent by a semi-preparative high-performance liquid chromatography system equipped with a molecule size exclusion chromatography function to obtain the neoagarooligosaccharides having the degrees of polymerization from 2 to 22; and using a fraction collector to collect the neoagarooligosaccharides, and obtaining purified single neoagarooligosaccharide products with different molecular masses after a freeze-drying process is performed to the neoagarooligosaccharides.

The invention provides a gene chip for detecting various seawater cultured animal pathogenic bacteria and its application. The pathogenic bacteria include: Vibrio anguillarum, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio fischeri, Vibrio splendidus, Vibrio riverina, Vibrio vulnificus, Vibrio cholerae non-O1, Vibrio mimicus, Vibrio salmonicida, Aeromonas hydrophila, Aeromonas sobria, Aeromonas salmonicida, Edward tarda Bacillus, Edwardsiella catfish, Renalobacter salmonidum, Mycobacterium marinum, Pseudomonas, Streptococcus.

The invention belongs to the technical field of microorganisms, and particularly relates to an application of pseudoalteromonas fish killing in fish and shrimp culture. The strain is preserved in the China Center for Type Culture Collection in Wuhan on November 12, 2020, and the preservation number is CCTCC NO: M 2020731. The bacterial strain has broad-spectrum anti-vibrio activity, has strong antagonistic activity on Aeromonas salmonicida, can enhance expression of fish lysozyme, tumor necrosis factors and cell interleukin genes after being orally taken, can significantly improve the immune level of aquaculture animal body fluid, and in addition, the strain can also significantly increase the length of intestinal microvilli of aquaculture animals, has a trend of increasing muscle thickness and villi length, is beneficial to improving the intestinal function of the aquaculture animals, realizes the synergistic effect of antagonizing pathogenic bacteria, immunizing and promoting the intestinal function, and more effectively prevents and controls bacterial diseases in aquaculture.

A microbial organic-inorganic compound fertilizer and a preparation method thereof. The invention, on the one hand, provides an application of aeromonas salmonicida in preparation of a microbial fertilizer and provides a microbial compound fertilizer on the other hand. The microbial compound fertilizer comprises an aeromonas salmonicida inoculant, humic acid and an inorganic fertilizer, wherein the quantity of live bacteria in the inoculant is 1-5 hundred million per gram and a mass ratio of the humic acid to the inorganic fertilizer is 5:5. The microbial compound fertilizer is rich in nutrition and can significantly improve quality and increase yield of cucumbers. Compared with a conventional compound fertilizer, the microbial compound fertilizer can increase the yield by 28% and the content of VC by 37% with an equal usage amount. In addition, the microbial compound fertilizer can promote an excellent ecological environment to form at rhizosphere and proliferation of beneficial microorganisms, thereby being beneficial to growth of crops. The microbial compound fertilizer can also increase a utilization rate of chemical fertilizers, can activate potential nutrients in soil and help the potential nutrients to release, improves resistance and immunity of crops and inhibits plant diseases and insect pests.

The invention discloses a preparation method and application of a live Aeromonas salmonicidal vaccine preparation and a freeze-dried vaccine preparation, and belongs to the technical field of fishery bacterial gene deletion live attenuated vaccines. The invention includes a markerless gene deletion attenuated mutant strain HTAsf-5307 of a wild strain of Aeromonas salmonicida, a live Aeromonas salmonicida vaccine prepared by the strain, a preparation method and application. The live attenuated vaccine strain of Aeromonas salmonicida provided by the present invention lacks some fragments of the aromatic amino acid synthesis-related genes of the Aeromonas salmonicidal virus strains, and some fragments of some type III secretion-related coding genes, relative to Its wild strain has obvious low toxicity and product safety, and at the same time, it can effectively protect immunized fish from the virus strain caused by Aeromonas salmonicida. The live vaccine has remarkable immunization effect, and can well prevent and treat diseases caused by Aeromonas salmonicida in practical application.

The invention relates to bacillus velezensis MVCR2 which can improve the immunity of rainbow trout and has a remarkable inhibition effect on aeromonas salmonicida and vibrio anguillarum. The invention further relates to a compound bacterium preparation containing the bacillus velezensis MVCR2. The invention further relates to application of the bacillus velezensis MVCR2. The bacillus velezensis MVCR2 disclosed by the invention is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 2021507, and the preservation date is May 8, 2021, and the preservation number is CCTCC NO: M 2021507. The bacillus velezensis MVCR2 has a remarkable inhibition effect on aeromonas salmonicida and vibrio anguillarum, has an effective prevention and treatment effect on various aquaculture diseases caused by the aeromonas salmonicida and the vibrio anguillarum, and can improve the growth and immunity of rainbow trout, increase the survival rate of rainbow trout and reduce the death rate of freshwater rainbow trout in the sea.

The invention discloses a biological degradation technology of organic sewage. The biological degradation technology comprises the steps of adding aeromonas salmonicida into the organic sewage and uniformly stirring. The biological degradation technology disclosed by the invention has the following advantages: the operation is simple, the effects are good, the pollution to land caused by garbage leachate is reduced, only one small device needs to be used for filtration, and not only cost, but also time are saved.

The invention belongs to the technical field of biology and specifically discloses the application of galectin-8 derived from Siniperca chuatsi in preparation of a bacteriostatic agent. The amino acidsequence of the galectin-8 is shown as SEQ ID NO. 1. It is found that the Siniperca chuatsi galectin-8 (ScGal8) has important antibacterial activity on typical aquatic animal pathogens; and an antibacterial experiment shows that the galectin-8 has no antibacterial activity on Aeromonas salmonicida (A. Salmonicida) and Edwardsiella tarda (E. Tarda), but has a relatively obvious inhibition effect on Streptococcus agalactiae (S. agalactiae) and Flavobacterium cloumnare (F. cloumnare), so the fish galectin-8 (rScGal8) has good application prospects in the treatment of gill rot diseases of aquaticanimals caused by Flavobacterium cloumnare.

The invention discloses an aeromonas salmonicida SYBR Green I real-time quantitive PCR (Polymerase Chain Reaction) detection method. An aeromonas salmonicida fstA gene is adopted as a target gene, and the aeromonas salmonicida SYBR Green I real-time quantitive PCR detection method is established. An experiment proves that the method provided by the invention not only has the characteristics of high specificity, high sensitivity and good repeatability, but also has the advantages of convenience in operation, quickness in identification, objectivity in results and the like, can be used for quickly diagnosing and quantitively detecting aeromonas salmonicida, and provides a technological means for diagnosis, prevention and control of salmon and bulltrout furunculosis.

A method for screening an algal toxin degrading bacterium from viscera of hemibarbus maculatus in Lake Taihu belongs to the field of MC-LR degrading microbiology. Aiming at problems of harmful algae outbreak and serious pollution of algal toxin in a water body, MC-LR extracted from dry algae powder of blue-green algae in the water body of Lake Taihu is taken as the only carbon source and nitrogensource, a bacterial strain JZ-4, namely Aeromonas salmonicida subsp.salmonicida, having relatively high MC-LR degrading capacity is screened out from the viscera of hemibarbus maculatus in Lake Taihu,and the 7d degradation rate is 89.72%. The influence of different pH conditions and different MC-LR adding amounts on the MC-LR degrading capacity of the bacterial strain is observed and studied. Theinvention provides the novel algal toxin degrading bacterial strain, and the optimum MC-LR degrading conditions are observed and studied, thereby providing theory and application references for waterbody microcystis algal toxin treatment by bioanalysis.

The invention discloses a purification method of a cyclina sinensis finished product. The purification method comprises the following steps: washing an obtained cyclina sinensis finished product; removing sludge; putting into a temporary cultivation tank; during a cultivation period, adding a microalgae preparation into sand-filtered seawater, wherein the microalgae is a mixture of skeletonema, tetraselmis and dicrateria inornata which are mixed at a random ratio; after 5 hours, putting the cyclina sinensis finished product into another temporary cultivation tank; adding a microbial preparation into the sand-filtered seawater, wherein the microbial preparation contains agritol freeze-dried powder, bacillus subtilis freeze-dried powder, bacillus licheniformis freeze-dried powder, wild mushroom entity freeze-dried powder and diatomite; and culturing for 24-48 hours, renewing the water and further culturing for 4-8 hours to obtain a purified finished product. The purification method disclosed by the invention has the advantages of effectively removing heavy metals in the body of the cyclina sinensis within short time, having a quite good removal effect on escherichia coli, sphingomonas paucimobilis, vibrio and aeromonas salmonicida, and is simple to operate, short in purification time and extremely good in purification efficiency.

The invention discloses a turbot-sourced Aeromonas salmonicida BHAS-1 and application thereof. The Aeromonas salmonicida BHAS-1 is Aeromonas salmonicida subspecies, is separated from internal organs of turbot with red mouth, fin ray hyperemia and red, swollen and convex anus symptoms, carries 14 virulence genes, and is obviously different from reported Aeromonas salmonicida with different sources; The Aeromonas salmonicida BHAS-1 is named as Aeromonas salmonicida BHAS-1, is preserved in China Center for Type Culture Collection on March 24, 2021, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 2021264, and the Aeromonas salmonicida BHAS-1 is classified and named as Aeromonas salmonicida BHAS-1. When the Aeromonas salmonicida BHAS-1 is used for preparing vaccines, turbot aeromonas diseases can be effectively prevented and controlled, the abuse of antibiotic drugs is reduced, and the quality safety of aquatic products is guaranteed.

The invention discloses a method for preparing alga oligosaccharide with anticoagulation activity. Specifically, in the method, an alga oligosaccharide sample prepared from sargassum, gelidium amansii or green laver is hydrolyzed with an enzyme so as to obtain the alga oligosaccharide with anticoagulation activity, wherein the enzyme is produced by inducing an ocean strain of Pseudomonas vesicularis and / or Aeromonas salmonicida with the ocean polysaccharide from sargassum, gelidium amansii or green laver.

The invention provides aeromonas salmonicida from cold water rainbow trout. The preservation number of the aeromonas salmonicida is CCTCC NO: M2018849. The aeromonas salmonicida is used for preparinga vaccine. The aeromonas salmonicida is obtained by screening pathogenic cold-water rainbow trout, and compared with existing aeromonas salmonicida, the strain has variation and is used for preparinga vaccine, and accordingly more perfect immune protection is provided for rainbow trout.

The invention relates to the field of biological detection, in particular to a probe, a primer group, a kit and a detection method for rapidly detecting the aeromonas salmonicida through RPA-LFS. According to the probe and the primer group for rapidly detecting the aeromonas salmonicida through the RPA-LFS, the sequence of the probe is shown as SEQ ID NO.1, and the sequences of the primer group are respectively SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses the detection method for detecting the aeromonas salmonicida, the reaction is rapid and sensitive, and the operation is simple.

The invention discloses a turbot-sourced aeromonas salmonicida attenuated strain and application thereof.The attenuated strain is a high-temperature derivative strain of aeromonas salmonicida for killing Japanese salmonella subspecies, namely aeromonas salmonicida AS-1, is classified and named as aeromonas salmonicida AS-1, is preserved in China Center for Type Culture Collection, the preservation address is Wuhan, China, and the preservation number is CGMCC NO.12487. The invention further discloses a method for preparing the turbot-sourced aeromonas salmonicida attenuated strain. The preservation date is February 18, 2022, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M 2022131. Through artificial infection, the aeromonas salmonicida AS-1 has low toxicity to scophthalmus maximus, a live vaccine prepared from the aeromonas salmonicida AS-1 is used for immune protection of the scophthalmus maximus, bacterial diseases caused by the fact that the aeromonas salmonicida kills subspecies Japanese salmonicida can be effectively prevented, and the aeromonas salmonicida AS-1 plays a positive role in reducing abuse of antibiotic drugs and guaranteeing quality safety of aquatic products.

The invention relates to an aeromonas salmonicida phage, a bactericidal composition comprising the same and an application of the bactericidal composition. The phage is capable of quickly inhibiting growth of Atlantic aeromonas salmonicida within a short period of time and inhibiting generation of resistance of the pathogenic bacterium phage. In addition, the bactericidal composition comprising the phage is safe and effective, and is high in specificity and low in production cost, and the disadvantages of single phagotherapy are compensated in the control process of pathogenic bacterium.