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Bacillus velezensis as well as compound bacteria preparation and application thereof

A technology of Bacillus velesi and Bacillus, which is applied to Bacillus velesi, the application of the above-mentioned Bacillus velesi MVCR2, and the field of compound bacterial preparations, which can solve fish immunity and quality changes, death, disease, loss, etc. To achieve the effect of inhibiting the growth of pathogenic bacteria, reducing mortality, and improving vitality

Pending Publication Date: 2022-04-29
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stress caused by changes in salinity during the domestication process can easily cause changes in fish immunity and quality, resulting in death or disease loss

Method used

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  • Bacillus velezensis as well as compound bacteria preparation and application thereof
  • Bacillus velezensis as well as compound bacteria preparation and application thereof
  • Bacillus velezensis as well as compound bacteria preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 strain screening and identification

[0038] Bacillus velezensis (Bacillus velezensis) MVCR2 of the present invention is finally isolated and screened from the intestinal tract of turbot by separating and screening the intestinal tracts of various aquatic animals such as Atlantic salmon, sea cucumber, rainbow trout, and turbot. The separation and screening method is as follows:

[0039] 1) Separation: Cut the intestinal tracts of Atlantic salmon, sea cucumber, rainbow trout, and turbot into pieces, take the pieces, add them into sterile saline, mix well, and incubate in a shaking incubator at 200 rpm for 15 minutes. After standing for 5 minutes, take the supernatant and heat it in an 80°C water bath for 10 minutes. Take 100 μL of the sample after the water bath and spread it on the LB solid medium plate; incubate at 37°C in a constant temperature incubator for 10-12 hours;

[0040] 2) Purification: Pick different single colonies from the cultured plate and d...

Embodiment 2

[0052] Example 2 Antagonism Experiment of Bacillus Velez MVCR2 and Bacillus Coagulans BNCC 136363

[0053] Bacillus Velez MVCR2 and Bacillus coagulans BNCC 136363 were streaked on the LB solid medium, cultured at 37°C for 12 hours, and single colonies were picked and crossed on the LB solid medium, and the streaked After the plates were cultured at 37°C for 24 hours, the growth of the strains was checked, and the results of the antagonism experiment streak culture showed that neither of the two strains had antagonism.

Embodiment 3

[0054] The bacteriostasis effect of embodiment 3 Veles bacillus MVCR2 and bacillus coagulans BNCC 136363 mixed bacterial solution

[0055] The bacteriostasis experiment operation steps and the amount of bacterial solution added are the same as in Example 1, wherein the mixed bacterial solution is composed of Bacillus Velez MVCR2 and Bacillus coagulans BNCC 136363 at a ratio of 2:1 for the number of viable bacteria. The results are shown in Table 2. From Table 2, it can be seen that the antibacterial effect of the mixed bacterial solution is significantly better than that of a single bacterial strain.

[0056] Table 2. Comparison of antibacterial experiments between single strain and mixed bacterial solution

[0057]

[0058] Note: -: no activity; +: diameter of inhibition zone 20mm .

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Abstract

The invention relates to bacillus velezensis MVCR2 which can improve the immunity of rainbow trout and has a remarkable inhibition effect on aeromonas salmonicida and vibrio anguillarum. The invention further relates to a compound bacterium preparation containing the bacillus velezensis MVCR2. The invention further relates to application of the bacillus velezensis MVCR2. The bacillus velezensis MVCR2 disclosed by the invention is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 2021507, and the preservation date is May 8, 2021, and the preservation number is CCTCC NO: M 2021507. The bacillus velezensis MVCR2 has a remarkable inhibition effect on aeromonas salmonicida and vibrio anguillarum, has an effective prevention and treatment effect on various aquaculture diseases caused by the aeromonas salmonicida and the vibrio anguillarum, and can improve the growth and immunity of rainbow trout, increase the survival rate of rainbow trout and reduce the death rate of freshwater rainbow trout in the sea.

Description

technical field [0001] The patent of the invention belongs to the field of microbes and their applications, and in particular relates to a strain of Bacillus Veles (MVCR2) that can improve the immunity of rainbow trout and has a significant inhibitory effect on Aeromonas salmonicida and Vibrio anguillarum; The invention also relates to a composite bacteria preparation containing the above-mentioned Bacillus velezensis MVCR2; meanwhile, the present invention also relates to the application of the above-mentioned Bacillus velezensis MVCR2. Background technique [0002] Rainbow trout is a high-quality cold-water fish. There are terrestrial and migratory types. The terrestrial type lives in fresh water all its life, and the migratory type lays eggs and hatches in fresh water. After growing up, it lives in seawater. Abroad, due to abundant cold water resources, rainbow trout are mainly cultured in fresh water. Since the introduction of rainbow trout in my country in 1971, it has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A23K10/18A23K50/80C12R1/07
CPCC12N1/20A01N63/22A23K10/18A23K50/80Y02A40/81
Inventor 任贻超门先会党永吉王博文陈佳旭
Owner QINGDAO AGRI UNIV
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