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Aeromonas salmonicida inactivated vaccine and applications thereof

A technology for killing Aeromonas salmonids and inactivating vaccines is applied in the directions of antibacterial drugs, medical preparations containing active ingredients, and antibody medical ingredients to achieve the effects of good safety, low cost and simple process.

Active Publication Date: 2016-11-09
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the Aeromonas salmonicida vaccines used in Europe are all prepared with the strain of Aeromonas salmonicida subsp.

Method used

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  • Aeromonas salmonicida inactivated vaccine and applications thereof
  • Aeromonas salmonicida inactivated vaccine and applications thereof
  • Aeromonas salmonicida inactivated vaccine and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] After the rejuvenation of Aeromonas salmonicida subspecies CGMCC No.7335, pick a single colony and inoculate it into LB liquid medium for 24-48 hours on a shaking table, add a final concentration of 0.3% formaldehyde to inactivate at room temperature for 48 hours, Centrifuge at 5000 rpm for 10 minutes, wash with sterile water, and resuspend the bacteria with sterile water to a concentration of 10 9 cells / ml, the inactivated vaccine antigen is obtained.

[0036] Take 19.2ml of the above-mentioned inactivated vaccine antigen suspension, add 0.8ml of sterilized Tween-80 and mix well to prepare the aqueous phase of the emulsified vaccine; then use 18.8ml of vaccine white oil, 1.2ml of Span-80, 0.4g of stearic acid Mix the aluminum phases, heat to completely dissolve the aluminum stearate, and sterilize to prepare the oil phase of the emulsified vaccine; use a magnetic stirrer at a speed of 5000 rpm, slowly add an equal volume of the oil phase to the water phase, and stir fo...

Embodiment 2

[0038] After the rejuvenation of Aeromonas salmonicida subspecies CGMCC No.7335, pick a single colony and inoculate it into LB liquid medium for 24-48 hours on a shaking table, add a final concentration of 0.3% formaldehyde to inactivate at room temperature for 48 hours, Centrifuge at 5000 rpm for 10 minutes, wash with sterile water, and resuspend the bacteria with sterile water to a concentration of 10 9 cells / ml, the inactivated vaccine antigen is obtained.

[0039]Take 19.6ml of the above-mentioned inactivated vaccine antigen suspension, add 0.4ml of sterilized Tween-80 and mix well to prepare the aqueous phase of the emulsified vaccine; then use 38.4ml of vaccine white oil, 1.6ml of Span-80, 0.6g of stearic acid Mix the aluminum phase, heat to completely dissolve the aluminum stearate, and sterilize to prepare the oil phase of the emulsified vaccine; use a disperser at a speed of 10,000 rpm, slowly add 40ml of the oil phase to 20ml of the water phase, and stir for 3 minute...

Embodiment 3

[0041] Step 1) Vaccination

[0042] According to the preparation method of the vaccine in the above-mentioned embodiment 1, the vaccine is prepared, and the inactivated vaccine antigen in the vaccine water phase is respectively provided with a high concentration of vaccine antigen (antigen cell concentration 1 × 10 8 cells / ml), low concentration vaccine antigen (antigen cell concentration 1×10 5 cells / ml) and an antigen-free vaccine without inactivated vaccine antigen (that is, the aqueous phase is sterile water and Tween-80), and an antigen-free vaccine was used as a control.

[0043] 300 Atlantic salmon (body weight 35g-40g) were randomly divided into 3 groups, 100 in each group. These three groups were named high concentration group, low concentration group and control group, respectively. Each Atlantic salmon in the high concentration group was intraperitoneally injected with 100 μl of antigen cells at a concentration of 1×10 8 cells / ml vaccine, intraperitoneally inject...

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Abstract

The invention belongs to the technical field of fish biological products and cultured fish disease prevention and control, and in particular relates to an aeromonas salmonicida inactivated vaccine and applications of the aeromonas salmonicida inactivated vaccine. The antigen of the inactivated vaccine is aeromonas salmonicida subsp.masoucida, and the bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 19, 2013, and is assigned with the accession number of CGMCC No.7335. The inactivated vaccine can effectively induce aquaculture fish to generate specific immune response, and provide effective protection, and a material for preparing the aeromonas salmonicida inactivated vaccine is low in cost and is safe to the environment and the host.

Description

technical field [0001] The invention belongs to the technical field of fish biological products and disease prevention and control of cultured fish, and in particular relates to an inactivated Aeromonas salmonicida vaccine and its application. Background technique [0002] Aeromonas salmonicida (Aeromonas salmonicida) is a Gram-negative facultative anaerobic bacteria belonging to the family Vibrio and the genus Aeromonas. Aeromonas salmonicida is an important fish pathogen with hosts including salmonids, cyprinids, pike, perch, and flounder, causing severe scabies / cankers in farmed and wild fish disease, resulting in massive fish kills. According to Bergey's Bacterial Classification Manual, Aeromonas salmonicida is divided into 5 subspecies, Aeromonassalmonicida subsp. . The fish diseases caused by different subspecies are different and have strong subspecies differences. The diseases caused by Aeromonas salmonicida subsp.salmonicida are generally called furunculosis. ),...

Claims

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Application Information

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IPC IPC(8): A61K39/106A61P31/04
CPCA61K39/107A61K2039/521A61K2039/545A61K2039/55
Inventor 肖鹏张丽娜孙斌王先平杜以帅孙明哲王顺奎于凯松刘鹰
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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