45 results about "Vibrio mimicus" patented technology

The invention produces high-performance specific gene amplification primers for detecting, quantifying, or identifying Vibrio cholerae and Vibrio mimicus, having low risk of misidentification and practically sufficient amplification efficiency and amplification specificity. We have determined partial nucleotide sequences of rpoD and gyrB genes of Vibrio cholerae, Vibrio mimicus, and closely related species, revealed their phylogenetic relationship, and then identified nucleotides characteristic of Vibrio cholerae and Vibrio mimicus, respectively. Thus, we have made it possible to design probes having high specificity and gene amplification primers having high specificity and excellent amplification efficiency, both of which contain the characteristic nucleotides.

The invention discloses a microdroplet digital PCR rapid detection method for mimetic vibrio in aquatic products. First, the genomic DNA of the enrichment solution of the aquatic product sample to be tested is extracted and diluted for use; then the conserved DNA sequence of the mimetic vibrio genome is used to design Specific primers and probes; then use the primers and probes to perform microdroplet digital PCR amplification and detection on the genomic DNA of the enriched bacteria solution of the aquatic product sample to be tested. After PCR amplification, the droplet analyzer was used to detect each droplet one by one. The droplet with fluorescent signal was interpreted as 1, and the droplet without fluorescent signal was interpreted as 0. Finally, according to the Poisson distribution principle and positive droplets The concentration or copy number of the target molecule to be detected is calculated. Droplet digital PCR can directly obtain the number of DNA molecules, and can perform absolute quantification of Vibrio mimicus in aquatic products. It has the advantages of high sensitivity, accurate quantification, wide linear range of detection, good specificity and moderate cost.