The invention provides a production process of sodium cholate, and the preparation method comprises the following steps: 1) collecting fresh gallbladder, cutting open the gallbladder skin, collecting the gall, stirring uniformly, adding sodium hydroxide and heating for melting; 2) cooling, siphoning, filtering, stirring and cooling again and precipitating; 3) adding acid and filtering, stirring and precipitating; 4) preparing granular cholic acid, washing, hanging to dryness, and drying to obtain a semi-finished product of sodium cholate; 5) guiding a mixture of alcohol and hydrogen peroxide into the semi-finished product of sodium cholate, stirring uniformly into a feed form, and spinning to dryness to obtain the sodium cholate. By adopting the production process provided by the invention, the production efficiency and the product quality are improved, and the production processes are reduced.