Method for preparing astragalus Sanxian soup flexible nano-liposome

Abstract

The invention discloses a method for preparing astragalus Sanxian soup flexible nano-liposome. The preparation method comprises the following steps: (1) weighing lecithin, cholesterol, astragaloside, icariin and notoginsenoside R1 according to a ratio, adding the raw materials into a container, and adding a methanol-chloroform mixed solvent, so that the raw materials are fully dissolved; (2) arranging the dissolved raw materials on a rotary evaporator, performing reduced pressure spin evaporation to remove an organic solvent in a constant temperature water bath, and performing vacuum drying overnight; (3) preparing a sodium cholate PBS solution, adding ethylene diamine tetramethylidene phosphoric acid into the sodium cholate PBS solution, adding the mixed solution into the dried raw materials, and performing normal pressure spin evaporation in the constant temperature water bath, thereby preparing primary suspension of the liposome; and (4) ultrasonically oscillating the primary suspension of the liposome, and finally sequentially squeezing the primary suspension to pass through microfiltration membranes with the pore diameters of 0.80mu m, 0.45mu m and 0.22mu m to obtain the flexible nano-liposome. The liposome disclosed by the invention has the advantages of uniform particle size, high encapsulation efficiency and high targeting property.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for preparing flexible nano-liposomes of Huangqi Sanxian Decoction. Background technique [0002] Osteoporosis is a systemic bone disease characterized by loss of bone mass and destruction of bone tissue microarchitecture due to uncoupling of bone resorption and bone formation, resulting in increased bone fragility and susceptibility to fracture. The World Health Organization defines osteoporosis as a decrease in bone mineral density (BMD) or bone mineral content (BMC) of more than 2.5 standard deviations from the mean of normal adult BMD. Osteoporosis is divided into primary osteoporosis and secondary osteoporosis according to the etiology. Clinical manifestations include pain, decreased bone density, shortened body length, kyphosis, decreased respiratory function, and fractures. With the prolongation of life expectancy and the increase of elderly population, osteopo...

AI Technical Summary

Problems solved by technology

The application of liposomes in the field of traditional Chinese medicine has also made gratifying achievements. Its characteristics of improving curative effect, reducing toxicity, targeting, sustained release, and cell affinity have been recognized, but there are still some problems in the research of liposomes in traditional Chinese medicine. : The research of liposomes of traditional Chinese medicine is mainly based on the research of liposomes of single active drug components, which is essentially the same as the research of liposomes of western medicines. Only liposomes with multiple components of traditional Chinese medicines can reflect the characteristics of Chinese medicines , also in line with the characteristics of traditional Chinese medicine

Some traditional Chinese medicine liposomes have low drug loading and poor stability, which needs to be further solved

Compared with western medicine, the complex and diverse chemical components of traditional Chinese medicine make the study of liposomes of traditional Chinese medicine much more difficult

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