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Preparation method of micro-emulsion kit

A kit and microemulsion technology, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc. problem, to achieve the effect of improving stability and large batch-to-batch variation

Active Publication Date: 2012-08-01
AILEX TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As an indicator of pancreatic function detection, serum lipase has good specificity. It has been recognized in clinical diagnosis. However, the reagents of the substrate colorimetric method used in the market have poor opening stability. The longer the opening time, the lower the result. The quality control dropped out of the range within half a month after opening. The refrigeration stability is relatively poor, and the substrate will precipitate after several months of refrigeration. In addition, the difference between batches is relatively large, so it brings a lot of inconvenience to the inspectors in use, and the reliability of the results has also declined.

Method used

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  • Preparation method of micro-emulsion kit
  • Preparation method of micro-emulsion kit
  • Preparation method of micro-emulsion kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The kit of the present invention includes the preparation of reagent R1 and the preparation of reagent R2, and the specific steps are as follows, wherein:

[0024] Preparation of reagent R1:

[0025] Take TAPS buffer 100mM / L; Colipase 0.8mg / L; sodium deoxycholate 1.6mM / L; sodium chloride 0.5g / L; calcium chloride 4mM / L; taurodeoxycholic acid 1.5mM / L; sodium azide 0.1g / L; the pH value of reagent R1 is 8.0.

[0026] Preparation of reagent R2:

[0027] A) get tartrate buffer solution 6mM / L; sodium cholate 4mM / L; taurodeoxycholic acid 7mM / L; preservative 0.1g / L; sodium chloride 0.5g / L; %-3%; the pH value of reagent R2 is 3.5.

[0028] B) Take 600 μl of organic solvent a n-butanol, add 0.0086 g of dodecyltrimethylammonium bromide, and add surfactant b, 3-[(3-cholesterylaminopropyl)dimethylamino]-1 - 0.0086 g of propanesulfonic acid, 10 mg of the substrate 1,2-dilauryl-rac-glyceryl-3-glutaric acid-6-methyl resorufin was added and dissolved in this solvent.

[0029] C) Aft...

Embodiment 2

[0031] The organic solvent a in Example 1 is replaced with isobutanol, and other configuration steps are the same as in Example 1.

Embodiment 3

[0033] Replace the surfactant b in Example 1 with Span 40, and the other configuration steps are the same as in Example 1.

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Abstract

The invention relates to a reagent and particularly relates to a method for preparing a stable micro-emulsion kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a TAPS buffered solution, Colipase, sodium deoxycholate, sodium chloride, calcium chloride, taurodeoxycholic acid and sodium azide; and the pH value of the reagent R1 is 8.0. The reagent R2 comprises a tartaric acid buffered solution, sodium cholate, taurodeoxycholic acid, a preservative, sodium chloride and a stabilizing agent; and the pH value of the reagent R2 is 3.5. Compared with the prior art, the improved emulsifying method can be used for solving the problems of poor stability and large batch difference of the reagent on the basis that the reagent does not influence other performances.

Description

[technical field] [0001] The invention relates to a reagent, in particular to a method for preparing a stable microemulsion kit, which can be used for the preparation of a serum lipase reagent in a substrate colorimetric method. [Background technique] [0002] As an indicator of pancreatic function detection, serum lipase has good specificity. It has been recognized in clinical diagnosis. However, the reagents of the substrate colorimetric method used in the market have poor opening stability. The longer the opening time, the lower the result. The quality control dropped out of the range within half a month after opening. The refrigeration stability is relatively poor, and the substrate will precipitate after several months of refrigeration. In addition, the difference between batches is relatively large, so it brings a lot of inconvenience to the inspectors in use, and the reliability of the results has also declined. [Content of the invention] [0003] In order to overc...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 李子樵张祥洪
Owner AILEX TECH GRP CO LTD
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