211 results about "Sodium Deoxycholate" patented technology

The invention relates to the field of safety monitoring of food microorganisms, and discloses a salmonella characteristic chromogenic liquid nutrient medium, a preparation method thereof and a rapid detection method of salmonella. The salmonella characteristic chromogenic liquid nutrient medium comprises the following main components: tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium phosphate, combined inhibitor, combined accelerator, characteristic enzymolysis substrate and cosolvent. The rapid detection method disclosed by the invention comprises two steps, i.e. pre-enrichment culture and chromogenic identification, and is characterized in that salmonella characteristic enzyme hydrolyzes corresponding substrates to result in that the culture medium is purple, thus rapidly judging the existence of salmonella; and the addition of the accelerator contributes to recovering damaged cells of salmonella and promoting growth of salmonella; and the added inhibitor can selectively inhibit the growth of other competitors so as to reduce the interference on detection by the competitors. The detection method disclosed by the invention has the advantages of short detection period, strong specificity and high accuracy, is simple to operate and is suitable for large-throughput detection of salmonella in food.

The present invention discloses one kind of freeze dried polyene phosphatidylcholine powder for injection and its preparation process. It features that the preparation contains polyene phosphatidylcholine and solubilizer sodium deoxycholate, sodium dehydrocholate or sodium cholate in the weight ratio of 0.5-1.5.

The invention pertains to the technical field of medicine, which more particularly relates to an elastic nano-vesicle preparation used for transitting the active ingredient paclitaxel (taxol) or docetaxel to penetrate the natural permeability barriers or pores (such as, skin, mucosa, tissues and organs, etc.) and a preparation method thereof. The preparation at least contains the following components with the weight percentages: 3 to 15 percent of phospholipid, 0.3 to 5 percent of sodium cholate or sodium deoxycholate, 0.01 to 5 percent of paclitaxel or docetaxel, 5 to 30 percent of alcohol and the rest of water or phosphate buffer solution. The formed elastic nano-vesicle has lipid double molecular layers, and the diameter of the typical elastic nano-vesicle is less than 200nm. The preparation is used for the treatment of malignant tumors, and the invention can be taken by injection, spray or transdermal drug delivery.

The invention discloses a polyene phosphatidyl choline freeze dried injection for the treatment of hepatic diseases, which comprises polyene phosphatidyl choline as the main ingredients and pharmaceutically acceptable carrying agents, wherein the carrying agent can be auxiliary solvent selected from sodium cholate, sodium deoxycholate, sodium deoxycholate, hydroxypropyl-beta-cyclodextrin, hydroxyethyl-beta-cyclodextrin and/or methyl cyclodextrin. The injection has higher stability.

The invention relates to a reagent and particularly relates to a method for preparing a stable micro-emulsion kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a TAPS buffered solution, Colipase, sodium deoxycholate, sodium chloride, calcium chloride, taurodeoxycholic acid and sodium azide; and the pH value of the reagent R1 is 8.0. The reagent R2 comprises a tartaric acid buffered solution, sodium cholate, taurodeoxycholic acid, a preservative, sodium chloride and a stabilizing agent; and the pH value of the reagent R2 is 3.5. Compared with the prior art, the improved emulsifying method can be used for solving the problems of poor stability and large batch difference of the reagent on the basis that the reagent does not influence other performances.

The invention relates to a loratadine-ambroxol pharmaceutical composite and a liposome solid preparation thereof and a preparation method thereof; the liposome comprises the following components according to the parts by weight percent: 1 part of loratadine, 5 parts of ambroxol hydrochloride, 3-30 parts of yolk lecithin, 1-14 of cholesterol, 1.2-10 parts of sodium deoxycholate and 3-18 parts of poloxamer 188.

The invention discloses a fluorescence quantitative PCR detection method for escherichia coli O157:H7. aA pair of specific primers amplified with a 20 bp section is designed aiming at the flic of escherichia coli O157:H7. The detection method comprises the following steps: firstly, separating escherichia coli O157:H7 from an actual sample by adopting a magnetic bead enriching method, then eliminating the disturbance brought by dead bacteria in the food sample by the treatment of sodium deoxycholate (SD) and propidium monoazide (PMA), and finally amplifying the live bacteria through a PCR amplification method. The detection method has the advantages of high sensitivity, accurate quantitation, strong specificity, short detection time, simple operation process, and no disturbance caused by the residual DNA or dead bacteria in the food sample.

The invention discloses an in-situ adipocyte composite inhibitor for the local weight loss and body care of human bodies and a preparation method thereof. The compound (composite inhibitor) comprises the following bulk drugs: anti-NPY monoclonal antibodies, alpha-aminoethanesulfonic acid, mannitol, enol phosphatidylcholine, sodium deoxycholate, trans-10, cis-12conjugated linoleic acid, docosahexenoic acid, berberine, emodin, sodium bicarbonate and injection water. The compound of the invention can synchronously inhibit the propagation and the differentiation of preadipocytes, accelerate and promote the decomposition and the conversion of fats in mature adipocytes, effectively reduce the quantity of in-situ and newborn mature adipocytes, regulate the expression, the synthesis and the secretion of relevant adipogenic genes and adipocyte growth factors from the gene level and the like, and control the propagation and the fat synthesis of the adipocytes at the source; and, additionally, the absorption utilization rate of the compound is improved through local administration, so the compound has the rapid and efficient weight loss effect.

The invention discloses a drug carrier of apigenin and a preparation method. The drug carrier is micro-emulsion prepared by mixing sodium deoxycholate, sucrose stearate, isopropyl alcohol, ethyl acetate and a sodium chloride water solution. The drug carrier disclosed by the invention takes the ethyl acetate as an oil phase and the apigenin has highest anti-oxidization activity and maximum accumulative releasing rate.

The invention relates to a method for preparing molybdenum disulfide-doped graphene fibers. The method comprises the following steps: dispersing graphite oxide and molybdenum disulfide in water, and performing ultrasonic treatment, thereby obtaining molybdenum disulfide and graphite oxide dilute dispersion; adding sodium deoxycholate, and stirring, thereby obtaining sodium deoxycholate/molybdenum disulfide-graphene oxide gel; extruding the sodium deoxycholate/molybdenum disulfide-graphene oxide gel in anhydrous ethyl alcohol for washing by using an injector, and sucking the anhydrous ethyl alcohol, thereby obtaining graphene oxide fibers after the anhydrous ethyl alcohol is completely evaporated; and adding hydroiodic acid for reacting, washing, and drying, thereby obtaining the molybdenum disulfide-doped graphene fibers. The method disclosed by the invention is simple in process and is easy for industrial production, the prepared molybdenum disulfide-doped graphene fibers are high in conductivity, and have high flexibility and have huge application prospects in the fields of energy storage devices, photovoltaic devices and sensors.

The invention discloses a megestrol acetate nanosuspension.The megestrol acetate nanosuspension is prepared by mixing megestrol acetate and a stabilizer solution and then grinding the mixture through a nanometer grinder.The stabilizer is a mixture obtained by mixing cellulose derivatives and anionic surfactants according to the mass ratio of 10: (1-2); the cellulose derivatives are hydroxypropyl methyl cellulose and hydroxyl propyl cellulose, and the mass ratio of hydroxypropyl methyl cellulose to hydroxyl propyl cellulose is 10: (1-10); the anionic surfactants are lauryl sodium sulfate and sodium deoxycholate, and the mass ratio of lauryl sodium sulfate to sodium deoxycholate is 10: (1-5).The invention further discloses a preparation method and application of the megestrol acetate nanosuspension.According to the megestrol acetate nanosuspension, the average grain diameter of megestrol acetate is below 1,000 nm, the dissolution rate of megestrol acetate is remarkably increased, and then the oral bioavailability of megestrol acetate is improved.

The invention discloses a preparation method of acellular matrix hydrogel. The preparation method comprises the following steps: obtaining a tissue block; transferring the tissue block into a solutioncontaining 0.02% of pancreatin and 0.05% of ethylenediamine tetraacetic acid, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 3% of Triton X, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 4% of sodium deoxycholate, and performing stirring for 2-3 h; moving the tissue block into deionized water to be soaked for 12-18 h to obtain a decellularized scaffold; transferring the decellularized scaffold into 75% alcohol to be soaked for 30 min, transferring the decellularized scaffold into a solution containing 0.1% peracetic acid, and carrying out stirring for 2-3 h; freeze-drying the decellularized scaffold, grinding the decellularized scaffold into matrix powder, transferring the matrix powder into a solution containing pepsase hydrochloride, and performing stirring for digestion for 2-3 d to obtain pre-gel; and diluting the pre-gel to a preset concentration, and performing standing to obtain the acellular matrix hydrogel. The acellular matrix hydrogel is efficiently extracted from umbilical cord tissue, and the integrity of the internal structure of the acellular matrix is reserved to the maximum extent.

The invention relates to preparation of a docetaxel-oleic acid prodrug and application of a nanostructure lipid carrier based on a core-match mechanism to drug delivery. The prodrug takes docetaxel as a parent drug and oleic acid as an aliphatic chain and is linked by a fat key. The nanostructure lipid carrier based on the core-match mechanism takes glycerol monostearate as solid lipid, the oleic acid as liquid lipid, Poloxamer 188 or sodium deoxycholate as a surface active agent and PLV2000 or PLV5000 as a modified material and encapsulates the docetaxel-oleic acid prodrug. The drug loading capacity of the nanostructure lipid carrier prepared is about 23 percent, and the drug loading capacity is obviously improved compared with that (5 percent) of the nanostructure lipid carrier encapsulating the docetaxel; the nanostructure lipid carrier is better in colloidal stability and more excellent in slow controlled release effect. A pharmacokinetic experiment can prove that the nanostructure lipid carrier based on the core-match mechanism can obviously improve the oral bioavailability of the docetaxel, and the docetaxel-oleic acid prodrug is good in stability, high in safety, suitable for oral administration and wider in market application prospect.

The invention relates to antitumor drugs and discloses anti-glioma drugs based on Venenum Bufonis extract and a preparation method thereof, the drugs being a bufotalin submicron emulsion and a bufotalin nanoparticle preparation, wherein the bufotalin submicron emulsion comprises Venenum Bufonis extract, medium chain triglyceride, a lecithin, poloxamer 188, glycerol, sodium oleate and injection water; the bufotalin nanoparticle preparation comprises Venenum Bufonis extract, glycerol monostearate, medium-chain fatty acid glyceride, oleic acid, a lecithin, poloxamer 188, sodium deoxycholate and injection water. Both the two preparations can combine with endothelial cells in cerebral blood capillaries, enabling the drugs to be transmitted into the brain through membranes or by means of adsorption, medication, endocytosis and transferring, cerebral targeting of the drugs is improved, and further increase of bufotalin in the brain is facilitated, and glioma resistance is achieved by inducing apoptosis and excessive autophagy of cells.

The invention provides a purification method for split influenza virus vaccine. An influenza virus strain is inoculated onto a chick embryo and cultured to obtain a virus solution; the virus solution is sequentially subjected to inactivation and ultrafiltration concentration to obtain an ultrafiltrate; the obtained ultrafiltrate is subjected to cane sugar density gradient centrifugation by using a KII continuous flow centrifuge to obtain an ultra centrifugal solution; the ultra centrifugal solution is subjected to ultrafiltration dialysis for cane sugar removal and molecular sieve gel chromatography to obtain a virus purification solution; the obtained virus purification solution is subjected to virus split by using a split agent TritonX-100 and sodium deoxycholate; after the split is over, the split agent is removed via ultrafiltration dialysis; impure protein is centrifugally removed from the obtained split solution; supernatant is collected, filtered and sterilized so as to obtain the purified influenza virus vaccine primary solution. The purification method is simple and convenient to operate, high in centrifugation capacity and suitable for large-scale production; by adopting a dual-split agent and a centrifugal process, the finished product is greatly improved in activity (hemagglutinin content/protein total content), and approaches the activity ratio of subunit vaccine, as a result, the high-grade split influenza virus vaccine product is obtained.