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Lipidosome composition containing Cu and Zn-rhSOD for removing eye wrinkles effectively

A technology of liposome composition and flexible liposome, which is applied in skin care preparations, cosmetics, cosmetic preparations, etc., and can solve the problems of not easy to penetrate the stratum corneum and cell membrane of the skin, easy inactivation, SOD inactivation, etc. , to achieve the daily skin care needs of anti-wrinkle, solve or prevent eye wrinkles, and prevent cell aging

Active Publication Date: 2013-12-04
上海玉华生命科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of SOD as an active enzyme as a cosmetic ingredient is affected by many factors, such as poor thermal stability, easy inactivation, and short half-life; the molecular weight is around 32,000, and it is difficult to penetrate the stratum corneum and cell membrane of the skin. These factors cause less effective
In order to solve the problem of stability and penetrating the cuticle and cell membrane of the skin, people try to use liposome technology, but it is difficult for the general liposome to penetrate the cuticle of the skin, and the liposome is easily broken during the freeze-drying process. lead to inactivation of SOD

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation of flexible liposome

[0021] Preparation of flexible liposomes by film-extrusion method

[0022] (1) Take a clean eggplant-shaped bottle and ensure it is fully dry and anhydrous. Precisely weigh soybean lecithin, sodium deoxycholate, and cholesterol in a 50ml eggplant-shaped bottle, add an appropriate amount of absolute ethanol, and ultrasonically dissolve in a short-term water bath. Accurately weigh an appropriate amount of SOD powder, EGF, and protein active protection agent, and dissolve them in 10ml of PBS solution.

[0023] (2) The organic phase containing the lipid material was transferred to a rotary evaporator for about 2 hours, and the temperature of the water bath was maintained at 40° C. until a uniform and continuous lipid film was obtained at the bottom of the eggplant-shaped flask. The eggplant-shaped bottle was transferred to a brown desiccator, and vacuumized overnight to fully remove residual organic solvents.

[0024] ...

Embodiment 2

[0032] Embodiment 2, preparation of Cu, Zn-rhSOD liposome freeze-dried powder containing Cu and Zn-rhSOD efficiently dispelling eye wrinkles

[0033] Based on the total cholesterol mass in the flexible liposome prepared in Example 1 (1), the contents of each component are shown in Table 2 (mass ratio)

[0034] Table 2

[0035] formula lyophilized excipient transdermal absorption enhancer Antioxidants liposomal total cholesterol 1 0.2 0.3 1 1 2 0.5 1 3 1 3 0.1 2 2 1 4 0.2 0.3 1 1 5 2.5 0.3 1 1 6 2.5 0.3 1 1 7 0.2 0.3 1 1

[0036] 1-5 is the flexible liposome suspension prepared by formula 3 of embodiment 1, and 6 and 7 are the flexible liposome suspension prepared by formula 5 of embodiment 1 (excluding ectoine);

[0037] Described lyophilized excipient: 1-4, 7 are hyaluronic acid; 5-6 are trehalose;

[0038] Described transdermal absorption enhancer: 1,5-7 is laurocapram; 2 is clove volatile oil, 3...

Embodiment 3

[0048] Embodiment 3, in vitro transdermal absorption experiment

[0049] With reference to the preparation method of Example 2, prepare liposome freeze-dried powder according to Table 3, take an appropriate amount to dissolve in water respectively, and then evenly spread it on the pigskin of the Franz diffusion cell, take an appropriate amount of the receiving solution after 12 hours, and detect the SOD enzyme activity and EGF content.

[0050] Based on the total cholesterol mass in the flexible liposome prepared by Formula 3 of Example 1 (1), the contents of each component are as follows in Table 3 (mass ratio)

[0051] table 3

[0052] formula ectoine transdermal absorption enhancer Vc alkyl phosphate 1 0.2 0.3 1 2 0.2 0.3 1 3 0.2 0.3 1 4 0.2 0 1

[0053] The transdermal absorption enhancer: 1 is laurocapram; 2 is clove volatile oil + peppermint oil (mass ratio is 1:1), 3 is the combination of clove volatile oil, peppermint oil...

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Abstract

The invention relates to the technical field of cosmetics, in particular to a lipidosome composition containing Cu and Zn-rhSOD for removing eye wrinkles effectively. The lipidosome composition comprises a freeze-dried excipient, a skin penetration enhancer, an antioxidant, and a flexible liposome coating Cu, Zn-rhSOD and EGF, and is characterized in that the flexible liposome comprises the following components in parts by weight: 80 to 95 parts of lecithin, 5 to 20 parts of sodium deoxycholate, 2 to 5 parts of cholesterol, 0.01 to 0.05 part of Cu and Zn-rhSOD, 0.01 to 0.03 part of EGF, and 5 to 10 parts of an active protective agent. The lipidosome composition provided by the invention can effectively remove or prevent eye wrinkles, and ensure that skin is relatively fine, smooth, tender, white and resilient, thereby meeting the daily skin care requirements of whitening and wrinkle removing of eye skin of people.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a liposome composition containing Cu and Zn-rhSOD for effectively removing eye wrinkles. Background technique [0002] The eye skin is one of the thinnest skins in the human body. The thickness of the surface layer of the eye skin is only 1 / 3-1 / 5 of that of the face; it blinks about 10,000 times a day and uses 14 groups of muscles around the eyes; in general, the sweat glands in the skin secrete The hydrolipidic film composed of sweat and fat is acidic, which can prevent dehydration, lubricate the skin, and resist part of the external environment's damage to the skin. It is the natural protective film of the skin, and there are only sebaceous glands and abnormal sweat glands around the eyes. Sweat glands have no distribution of sebum, so the hydrolipidic film has little protective effect on the eye skin. In addition, the eye skin has less fat content, and the dermis has less e...

Claims

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Application Information

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IPC IPC(8): A61K8/66A61K8/64A61K8/73A61K8/97A61K8/49A61K8/14A61Q19/08A61Q19/02
Inventor 刘霞
Owner 上海玉华生命科技发展有限公司
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