The invention relates to the technical fields of
tissue engineering and medical wound repair. At present, a living
skin substitute constructed by using the materials of
polylactic acid, polyglycolic acid, collagen,
hyaluronic acid, and the like as a dermic bracket has the defects that on one hand, host materials are difficult to extract, the living
skin substitute has complicated
manufacturing technology and is expensive in cost and difficult to widely popularize and apply clinically; and on the other hand, the living
skin substitute does not have a skin
basilar membrane structure so that healed skin does not
resist pressure and wear, and the living skin has unfirm adhesion to the epidermis and is easy to shed and break or form water
blisters so that the structural and morphological development of the normal epidermis is influenced; and allogeneic
acellular dermis is taken from
cadaver skin and is limited in sources and expensive in cost, and thus clinical application is limited. The invention aims at providing a skin substitute which uses surface-finished and modified
amnion as the
basilar membrane and
blood plasma as stroma, which has the advantages of wide material sources, low cost and simple preparation method. An animal experiment proves that the complete
basilar membrane and hemidesmosomes can be retained in in-vivo
transplantation, and the formation of an epidermal structural form is accelerated and promoted.