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Method for preparing acellular dermal matrix by utilizing ultrasonic wave

An acellular dermis and ultrasonic technology, applied in the field of medical skin tissue engineering, can solve the problems of long processing time, multi-chemical substances, complicated preparation process, etc., and achieve the effects of simple and practical preparation process, short production cycle and environmental friendliness

Inactive Publication Date: 2011-10-26
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, Chen Bi and others were the first to conduct experimental and clinical research on allogeneic dermis decellularization, and a similar product of Alloderm is currently on the market. However, due to the limited source of human allodermal skin and the ethical issues involved, it cannot meet the clinical needs. Therefore, Xenogeneic acellular dermal matrix becomes an effective way to solve this contradiction
[0005] However, although the existing heterogeneous acellular dermal matrix preparation is relatively mature, there are many problems in the preparation process, such as: the preparation process is complicated, the processing time is too long Long, the method used is more destructive to the ultrastructure of the natural dermis, the extracellular matrix components are poorly preserved, and more chemical substances are left.

Method used

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  • Method for preparing acellular dermal matrix by utilizing ultrasonic wave
  • Method for preparing acellular dermal matrix by utilizing ultrasonic wave
  • Method for preparing acellular dermal matrix by utilizing ultrasonic wave

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Preparation of heterogeneous acellular dermal matrix

[0040] (1) Peel off the healthy newborn cow skin and shave off the hair;

[0041] (2) Wash and soak with 0.1% benzalkonium bromide solution, sterilize the skin with povidone iodine for 5 minutes, and use a skin drum to take the reticular dermis with a thickness of 0.2 mm under aseptic conditions;

[0042] (3) Shock the reticular dermis in distilled water for 2 hours, then place it in 1 M NaCl for 2 hours;

[0043] (4) Repeat step (3) 3 times;

[0044](5) Put the reticulated dermis in 0.5% Triton X-100 and treat it with an ultrasonic cleaner for 24 hours. The frequency of the ultrasonic cleaner is set to 40 kHz and the power is 250 W;

[0045] (6) Wash 3 times with sterile phosphate buffered saline, 15 min each time, and sterilize with ethylene oxide to obtain the acellular dermal matrix.

Embodiment 2

[0046] Example 2, preparation of heterogeneous acellular dermal matrix

[0047] (1) Peel off the skin of healthy and clean SD rats, and shave off the hair;

[0048] (2) Wash and soak with 0.1% benzalkonium bromide solution, sterilize the skin with povidone iodine for 5 minutes, and use a skin drum to take the reticular dermis with a thickness of 0.3 mm under aseptic conditions;

[0049] (3) Shock the reticular dermis in distilled water for 1 hour, then place it in 1 M NaCl for 1 hour;

[0050] (4) Repeat step (3) 3 times;

[0051] (5) Treat the reticular dermis in 0.5% Triton X-100 with an ultrasonic cleaner for 12 hours, set the frequency to 40 kHz, and power to 250 W;

[0052] (6) Wash 3 times with sterile phosphate buffered saline, 15 min each time, and sterilize with ethylene oxide to obtain the acellular dermal matrix.

Embodiment 3

[0053] Example 3, preparation of heterogeneous acellular dermal matrix

[0054] (1) Peel off the healthy rabbit skin and shave off the hair;

[0055] (2) Wash and soak with 0.1% benzalkonium bromide solution, sterilize the skin with 75% alcohol for 5 minutes, and use a skin drum to take the reticular dermis with a thickness of 0.3 mm under aseptic conditions;

[0056] (3) Place the reticular dermis in a mixture of 10 mM Trizma HCl and 10 mM EDTA for 2 hours, and then place it in 1 M NaCl for 2 hours;

[0057] (4) Repeat step (3) 3 times;

[0058] (5) Put the reticulated dermis in 0.5% Tween-20 and treat it with an ultrasonic cleaner for 20 hours. The frequency of the ultrasonic cleaner is set to 30 kHz and the power is 250 W;

[0059] (6) Wash 3 times with sterile phosphate buffered saline, 15 min each time, and sterilize with ethylene oxide to obtain the acellular dermal matrix.

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Abstract

The invention discloses a method for preparing an acellular dermal matrix by utilizing ultrasonic wave. The method comprises the following steps of: removing hair from peeled mammal skin; disinfecting the skin with disinfectant, and preparing reticular-layer derma by adopting a mechanical method under the sterile condition; oscillating the derma in hyposmotic solution for 0.5-12 hours, and then oscillating the derma in hyperosmotic solution for 0.5-12 hours; repeating the steps 1-6 times; placing the derma into washing agent and processing with an ultrasonic cleaner for 12-48 hours; and washing with sterile phosphate buffer solution 1-6 times, and disinfecting with ethylene oxide to obtain the acellular dermal matrix. According to the method disclosed by the invention, the preparation process is simple and practical, cells can be thoroughly removed, and the production period is short; and the method is environmentally-friendly, has low cost and is applicable to large-scale operation.

Description

technical field [0001] The invention relates to the field of medical skin tissue engineering, in particular to a preparation method of heterogeneous acellular dermal matrix. Background technique [0002] Skin defects caused by burns, wounds, war injuries and other factors often leave scar hyperplasia and contracture deformity, which seriously affect the appearance and function. Loewe was the first to prove that dermal tissue can enhance the toughness of grafted skin, inhibit the growth of granulation tissue and scar formation, and reduce wound contracture degree. If the reconstruction of dermal structure is combined with other measures to promote wound healing, it can effectively reduce the formation of scars and improve the quality of wound healing. [0003] The key problem of the skin all the time is immune rejection, which is mainly derived from cellular components such as epidermal cells, fibroblasts, and endothelial cells. Acellular Dermal Matrix (ADM) is a dermal sub...

Claims

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Application Information

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IPC IPC(8): A61L27/60
Inventor 彭代智陈博
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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